Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo

Teunis B H Geijtenbeek, Peter C Groot, Martijn A Nolte, Sandra J van Vliet, Shanti T Gangaram-Panday, Gerard C F van Duijnhoven, Georg Kraal, Antoon J M van Oosterhout, Yvette van Kooyk

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Antigen-presenting cells are localized in essentially every tissue, where they operate at the interface of innate and acquired immunity by capturing pathogens and presenting pathogen-derived peptides to T cells. C-type lectins are important pathogen recognition receptors and the C-type lectin, dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN), is unique in that, in addition to pathogen capture, it regulates adhesion processes such as DC trafficking and T-cell synapse formation. We have isolated a murine homologue of DC-SIGN that is identical to the previously reported murine homologue mSIGNR1. mSIGNR1 is more closely related to the human DC-SIGN homologue L-SIGN than to DC-SIGN itself because mSIGNR1 is specifically expressed by liver sinusoidal endothelial cells, similar to L-SIGN, and not by DCs. Moreover, mSIGNR1 is also expressed by medullary and subcapsular macrophages in lymph nodes and by marginal zone macrophages (MZMs) in the spleen. Strikingly, these MZMs are in direct contact with the bloodstream and efficiently capture specific polysaccharide antigens present on the surface of encapsulated bacteria. We have investigated the in vivo function of mSIGNR1 on MZMs in spleen. We demonstrate here that mSIGNR1 functions in vivo as a pathogen recognition receptor on MZMs that capture blood-borne antigens, which are rapidly internalized and targeted to lysosomes for processing. Moreover, the antigen capture is completely blocked in vivo by the blocking mSIGNR1-specific antibodies. Thus, mSIGNR1, a murine homologue of DC-SIGN, is important in the defense against pathogens and this study will facilitate further investigations into the in vivo function of DC-SIGN and its homologues.

Original languageEnglish
Pages (from-to)2908-16
Number of pages9
JournalBlood
Volume100
Issue number8
DOIs
Publication statusPublished - 15 Oct 2002

Cite this

Geijtenbeek, Teunis B H ; Groot, Peter C ; Nolte, Martijn A ; van Vliet, Sandra J ; Gangaram-Panday, Shanti T ; van Duijnhoven, Gerard C F ; Kraal, Georg ; van Oosterhout, Antoon J M ; van Kooyk, Yvette. / Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo. In: Blood. 2002 ; Vol. 100, No. 8. pp. 2908-16.
@article{8d02b27fc5974a9698cf1ac748a8ff13,
title = "Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo",
abstract = "Antigen-presenting cells are localized in essentially every tissue, where they operate at the interface of innate and acquired immunity by capturing pathogens and presenting pathogen-derived peptides to T cells. C-type lectins are important pathogen recognition receptors and the C-type lectin, dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN), is unique in that, in addition to pathogen capture, it regulates adhesion processes such as DC trafficking and T-cell synapse formation. We have isolated a murine homologue of DC-SIGN that is identical to the previously reported murine homologue mSIGNR1. mSIGNR1 is more closely related to the human DC-SIGN homologue L-SIGN than to DC-SIGN itself because mSIGNR1 is specifically expressed by liver sinusoidal endothelial cells, similar to L-SIGN, and not by DCs. Moreover, mSIGNR1 is also expressed by medullary and subcapsular macrophages in lymph nodes and by marginal zone macrophages (MZMs) in the spleen. Strikingly, these MZMs are in direct contact with the bloodstream and efficiently capture specific polysaccharide antigens present on the surface of encapsulated bacteria. We have investigated the in vivo function of mSIGNR1 on MZMs in spleen. We demonstrate here that mSIGNR1 functions in vivo as a pathogen recognition receptor on MZMs that capture blood-borne antigens, which are rapidly internalized and targeted to lysosomes for processing. Moreover, the antigen capture is completely blocked in vivo by the blocking mSIGNR1-specific antibodies. Thus, mSIGNR1, a murine homologue of DC-SIGN, is important in the defense against pathogens and this study will facilitate further investigations into the in vivo function of DC-SIGN and its homologues.",
keywords = "Amino Acid Sequence, Animals, Antigens/blood, Antigens, CD/genetics, Base Sequence, Bone Marrow Cells/cytology, Cell Adhesion/immunology, Cell Adhesion Molecules/genetics, DNA Primers, Dendritic Cells/immunology, Enzyme-Linked Immunosorbent Assay, Humans, Lectins, C-Type/genetics, Macrophages/immunology, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Receptors, Cell Surface/genetics, Recombinant Fusion Proteins/immunology, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Homology, Amino Acid, Species Specificity",
author = "Geijtenbeek, {Teunis B H} and Groot, {Peter C} and Nolte, {Martijn A} and {van Vliet}, {Sandra J} and Gangaram-Panday, {Shanti T} and {van Duijnhoven}, {Gerard C F} and Georg Kraal and {van Oosterhout}, {Antoon J M} and {van Kooyk}, Yvette",
year = "2002",
month = "10",
day = "15",
doi = "10.1182/blood-2002-04-1044",
language = "English",
volume = "100",
pages = "2908--16",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "8",

}

Geijtenbeek, TBH, Groot, PC, Nolte, MA, van Vliet, SJ, Gangaram-Panday, ST, van Duijnhoven, GCF, Kraal, G, van Oosterhout, AJM & van Kooyk, Y 2002, 'Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo' Blood, vol. 100, no. 8, pp. 2908-16. https://doi.org/10.1182/blood-2002-04-1044

Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo. / Geijtenbeek, Teunis B H; Groot, Peter C; Nolte, Martijn A; van Vliet, Sandra J; Gangaram-Panday, Shanti T; van Duijnhoven, Gerard C F; Kraal, Georg; van Oosterhout, Antoon J M; van Kooyk, Yvette.

In: Blood, Vol. 100, No. 8, 15.10.2002, p. 2908-16.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo

AU - Geijtenbeek, Teunis B H

AU - Groot, Peter C

AU - Nolte, Martijn A

AU - van Vliet, Sandra J

AU - Gangaram-Panday, Shanti T

AU - van Duijnhoven, Gerard C F

AU - Kraal, Georg

AU - van Oosterhout, Antoon J M

AU - van Kooyk, Yvette

PY - 2002/10/15

Y1 - 2002/10/15

N2 - Antigen-presenting cells are localized in essentially every tissue, where they operate at the interface of innate and acquired immunity by capturing pathogens and presenting pathogen-derived peptides to T cells. C-type lectins are important pathogen recognition receptors and the C-type lectin, dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN), is unique in that, in addition to pathogen capture, it regulates adhesion processes such as DC trafficking and T-cell synapse formation. We have isolated a murine homologue of DC-SIGN that is identical to the previously reported murine homologue mSIGNR1. mSIGNR1 is more closely related to the human DC-SIGN homologue L-SIGN than to DC-SIGN itself because mSIGNR1 is specifically expressed by liver sinusoidal endothelial cells, similar to L-SIGN, and not by DCs. Moreover, mSIGNR1 is also expressed by medullary and subcapsular macrophages in lymph nodes and by marginal zone macrophages (MZMs) in the spleen. Strikingly, these MZMs are in direct contact with the bloodstream and efficiently capture specific polysaccharide antigens present on the surface of encapsulated bacteria. We have investigated the in vivo function of mSIGNR1 on MZMs in spleen. We demonstrate here that mSIGNR1 functions in vivo as a pathogen recognition receptor on MZMs that capture blood-borne antigens, which are rapidly internalized and targeted to lysosomes for processing. Moreover, the antigen capture is completely blocked in vivo by the blocking mSIGNR1-specific antibodies. Thus, mSIGNR1, a murine homologue of DC-SIGN, is important in the defense against pathogens and this study will facilitate further investigations into the in vivo function of DC-SIGN and its homologues.

AB - Antigen-presenting cells are localized in essentially every tissue, where they operate at the interface of innate and acquired immunity by capturing pathogens and presenting pathogen-derived peptides to T cells. C-type lectins are important pathogen recognition receptors and the C-type lectin, dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN), is unique in that, in addition to pathogen capture, it regulates adhesion processes such as DC trafficking and T-cell synapse formation. We have isolated a murine homologue of DC-SIGN that is identical to the previously reported murine homologue mSIGNR1. mSIGNR1 is more closely related to the human DC-SIGN homologue L-SIGN than to DC-SIGN itself because mSIGNR1 is specifically expressed by liver sinusoidal endothelial cells, similar to L-SIGN, and not by DCs. Moreover, mSIGNR1 is also expressed by medullary and subcapsular macrophages in lymph nodes and by marginal zone macrophages (MZMs) in the spleen. Strikingly, these MZMs are in direct contact with the bloodstream and efficiently capture specific polysaccharide antigens present on the surface of encapsulated bacteria. We have investigated the in vivo function of mSIGNR1 on MZMs in spleen. We demonstrate here that mSIGNR1 functions in vivo as a pathogen recognition receptor on MZMs that capture blood-borne antigens, which are rapidly internalized and targeted to lysosomes for processing. Moreover, the antigen capture is completely blocked in vivo by the blocking mSIGNR1-specific antibodies. Thus, mSIGNR1, a murine homologue of DC-SIGN, is important in the defense against pathogens and this study will facilitate further investigations into the in vivo function of DC-SIGN and its homologues.

KW - Amino Acid Sequence

KW - Animals

KW - Antigens/blood

KW - Antigens, CD/genetics

KW - Base Sequence

KW - Bone Marrow Cells/cytology

KW - Cell Adhesion/immunology

KW - Cell Adhesion Molecules/genetics

KW - DNA Primers

KW - Dendritic Cells/immunology

KW - Enzyme-Linked Immunosorbent Assay

KW - Humans

KW - Lectins, C-Type/genetics

KW - Macrophages/immunology

KW - Mice

KW - Mice, Inbred BALB C

KW - Molecular Sequence Data

KW - Receptors, Cell Surface/genetics

KW - Recombinant Fusion Proteins/immunology

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Alignment

KW - Sequence Homology, Amino Acid

KW - Species Specificity

U2 - 10.1182/blood-2002-04-1044

DO - 10.1182/blood-2002-04-1044

M3 - Article

VL - 100

SP - 2908

EP - 2916

JO - Blood

JF - Blood

SN - 0006-4971

IS - 8

ER -