Maximal oxygen uptake is proportional to muscle fiber oxidative capacity - from chronic heart failure patients to professional cyclists

S. van der Zwaard, C.J. de Ruiter, D.A. Noordhof, R. Sterrenburg, F.W. Bloemers, J.J. de Koning, R.T. Jaspers, W.J. van der Laarse

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

VO2max during whole-body exercise is presumably constrained by oxygen delivery to mitochondria rather than by mitochondria's ability to consume oxygen. Humans and animals have been reported to exploit only 60-80% of their mitochondrial oxidative capacity at VO2max. However, ex vivo quantification of mitochondrial overcapacity is complicated by isolation or permeabilization procedures. An alternative method for estimating mitochondrial oxidative capacity is via enzyme histochemical quantification of succinate dehydrogenase (SDH) activity. We determined to what extent V̇O2max attained during cycling exercise differs from mitochondrial oxidative capacity predicted from SDH activity of m. vastus lateralis in chronic heart failure patients, healthy controls and cyclists. VO2max was assessed in 20 healthy subjects and 28 cyclists and SDH activity was determined from biopsy cryosections of m. vastus lateralis using quantitative histochemistry. Similar data from our laboratory of 14 chronic heart failure patients and 6 controls were included. Mitochondrial oxidative capacity was predicted from SDH activity using estimated skeletal muscle mass and the relationship between ex vivo fiber VO2max and SDH activity of isolated single muscle fibers and myocardial trabecula under hyperoxic conditions. Mitochondrial oxidative capacity predicted from SDH activity was related (r2=0.89, p
Original languageEnglish
Pages (from-to)636-645
Number of pages10
JournalJournal of Applied Physiology
Volume121
Issue number3
DOIs
Publication statusPublished - 2016

Cite this

van der Zwaard, S. ; de Ruiter, C.J. ; Noordhof, D.A. ; Sterrenburg, R. ; Bloemers, F.W. ; de Koning, J.J. ; Jaspers, R.T. ; van der Laarse, W.J. / Maximal oxygen uptake is proportional to muscle fiber oxidative capacity - from chronic heart failure patients to professional cyclists. In: Journal of Applied Physiology. 2016 ; Vol. 121, No. 3. pp. 636-645.
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title = "Maximal oxygen uptake is proportional to muscle fiber oxidative capacity - from chronic heart failure patients to professional cyclists",
abstract = "VO2max during whole-body exercise is presumably constrained by oxygen delivery to mitochondria rather than by mitochondria's ability to consume oxygen. Humans and animals have been reported to exploit only 60-80{\%} of their mitochondrial oxidative capacity at VO2max. However, ex vivo quantification of mitochondrial overcapacity is complicated by isolation or permeabilization procedures. An alternative method for estimating mitochondrial oxidative capacity is via enzyme histochemical quantification of succinate dehydrogenase (SDH) activity. We determined to what extent V̇O2max attained during cycling exercise differs from mitochondrial oxidative capacity predicted from SDH activity of m. vastus lateralis in chronic heart failure patients, healthy controls and cyclists. VO2max was assessed in 20 healthy subjects and 28 cyclists and SDH activity was determined from biopsy cryosections of m. vastus lateralis using quantitative histochemistry. Similar data from our laboratory of 14 chronic heart failure patients and 6 controls were included. Mitochondrial oxidative capacity was predicted from SDH activity using estimated skeletal muscle mass and the relationship between ex vivo fiber VO2max and SDH activity of isolated single muscle fibers and myocardial trabecula under hyperoxic conditions. Mitochondrial oxidative capacity predicted from SDH activity was related (r2=0.89, p",
author = "{van der Zwaard}, S. and {de Ruiter}, C.J. and D.A. Noordhof and R. Sterrenburg and F.W. Bloemers and {de Koning}, J.J. and R.T. Jaspers and {van der Laarse}, W.J.",
year = "2016",
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Maximal oxygen uptake is proportional to muscle fiber oxidative capacity - from chronic heart failure patients to professional cyclists. / van der Zwaard, S.; de Ruiter, C.J.; Noordhof, D.A.; Sterrenburg, R.; Bloemers, F.W.; de Koning, J.J.; Jaspers, R.T.; van der Laarse, W.J.

In: Journal of Applied Physiology, Vol. 121, No. 3, 2016, p. 636-645.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van der Zwaard, S.

AU - de Ruiter, C.J.

AU - Noordhof, D.A.

AU - Sterrenburg, R.

AU - Bloemers, F.W.

AU - de Koning, J.J.

AU - Jaspers, R.T.

AU - van der Laarse, W.J.

PY - 2016

Y1 - 2016

N2 - VO2max during whole-body exercise is presumably constrained by oxygen delivery to mitochondria rather than by mitochondria's ability to consume oxygen. Humans and animals have been reported to exploit only 60-80% of their mitochondrial oxidative capacity at VO2max. However, ex vivo quantification of mitochondrial overcapacity is complicated by isolation or permeabilization procedures. An alternative method for estimating mitochondrial oxidative capacity is via enzyme histochemical quantification of succinate dehydrogenase (SDH) activity. We determined to what extent V̇O2max attained during cycling exercise differs from mitochondrial oxidative capacity predicted from SDH activity of m. vastus lateralis in chronic heart failure patients, healthy controls and cyclists. VO2max was assessed in 20 healthy subjects and 28 cyclists and SDH activity was determined from biopsy cryosections of m. vastus lateralis using quantitative histochemistry. Similar data from our laboratory of 14 chronic heart failure patients and 6 controls were included. Mitochondrial oxidative capacity was predicted from SDH activity using estimated skeletal muscle mass and the relationship between ex vivo fiber VO2max and SDH activity of isolated single muscle fibers and myocardial trabecula under hyperoxic conditions. Mitochondrial oxidative capacity predicted from SDH activity was related (r2=0.89, p

AB - VO2max during whole-body exercise is presumably constrained by oxygen delivery to mitochondria rather than by mitochondria's ability to consume oxygen. Humans and animals have been reported to exploit only 60-80% of their mitochondrial oxidative capacity at VO2max. However, ex vivo quantification of mitochondrial overcapacity is complicated by isolation or permeabilization procedures. An alternative method for estimating mitochondrial oxidative capacity is via enzyme histochemical quantification of succinate dehydrogenase (SDH) activity. We determined to what extent V̇O2max attained during cycling exercise differs from mitochondrial oxidative capacity predicted from SDH activity of m. vastus lateralis in chronic heart failure patients, healthy controls and cyclists. VO2max was assessed in 20 healthy subjects and 28 cyclists and SDH activity was determined from biopsy cryosections of m. vastus lateralis using quantitative histochemistry. Similar data from our laboratory of 14 chronic heart failure patients and 6 controls were included. Mitochondrial oxidative capacity was predicted from SDH activity using estimated skeletal muscle mass and the relationship between ex vivo fiber VO2max and SDH activity of isolated single muscle fibers and myocardial trabecula under hyperoxic conditions. Mitochondrial oxidative capacity predicted from SDH activity was related (r2=0.89, p

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