Measles virus targets DC-SIGN to enhance dendritic cell infection

Lot de Witte, Marion Abt, Sibylle Schneider-Schaulies, Yvette van Kooyk, Teunis B H Geijtenbeek

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Dendritic cells (DCs) are involved in the pathogenesis of measles virus (MV) infection by inducing immune suppression and possibly spreading the virus from the respiratory tract to lymphatic tissues. It is becoming evident that DC function can be modulated by the involvement of different receptors in pathogen interaction. Therefore, we have investigated the relative contributions of different MV-specific receptors on DCs to MV uptake into and infection of these cells. DCs express the MV receptors CD46 and CD150, and we demonstrate that the C-type lectin DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a novel receptor for laboratory-adapted and wild-type MV strains. The ligands for DC-SIGN are both MV glycoproteins F and H. In contrast to CD46 and CD150, DC-SIGN does not support MV entry, since DC-SIGN does not confer susceptibility when stably expressed in CHO cells. However, DC-SIGN is important for the infection of immature DCs with MV, since both attachment and infection of immature DCs with MV are blocked in the presence of DC-SIGN inhibitors. Our data demonstrate that DC-SIGN is crucial as an attachment receptor to enhance CD46/CD150-mediated infection of DCs in cis. Moreover, MV might not only target DC-SIGN to infect DCs but may also use DC-SIGN for viral transmission and immune suppression.

Original languageEnglish
Pages (from-to)3477-86
Number of pages10
JournalJournal of Virology
Volume80
Issue number7
DOIs
Publication statusPublished - Apr 2006

Cite this

de Witte, Lot ; Abt, Marion ; Schneider-Schaulies, Sibylle ; van Kooyk, Yvette ; Geijtenbeek, Teunis B H. / Measles virus targets DC-SIGN to enhance dendritic cell infection. In: Journal of Virology. 2006 ; Vol. 80, No. 7. pp. 3477-86.
@article{0ba9fc846f7b4878b60adeb753849394,
title = "Measles virus targets DC-SIGN to enhance dendritic cell infection",
abstract = "Dendritic cells (DCs) are involved in the pathogenesis of measles virus (MV) infection by inducing immune suppression and possibly spreading the virus from the respiratory tract to lymphatic tissues. It is becoming evident that DC function can be modulated by the involvement of different receptors in pathogen interaction. Therefore, we have investigated the relative contributions of different MV-specific receptors on DCs to MV uptake into and infection of these cells. DCs express the MV receptors CD46 and CD150, and we demonstrate that the C-type lectin DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a novel receptor for laboratory-adapted and wild-type MV strains. The ligands for DC-SIGN are both MV glycoproteins F and H. In contrast to CD46 and CD150, DC-SIGN does not support MV entry, since DC-SIGN does not confer susceptibility when stably expressed in CHO cells. However, DC-SIGN is important for the infection of immature DCs with MV, since both attachment and infection of immature DCs with MV are blocked in the presence of DC-SIGN inhibitors. Our data demonstrate that DC-SIGN is crucial as an attachment receptor to enhance CD46/CD150-mediated infection of DCs in cis. Moreover, MV might not only target DC-SIGN to infect DCs but may also use DC-SIGN for viral transmission and immune suppression.",
keywords = "Animals, Antigens, CD/metabolism, CHO Cells, Cell Adhesion Molecules/genetics, Cricetinae, Dendritic Cells/cytology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescein-5-isothiocyanate, Fluorescent Antibody Technique, Indirect, Fluorescent Dyes, Green Fluorescent Proteins/metabolism, Humans, K562 Cells, Lectins, C-Type/genetics, Ligands, Measles/virology, Measles virus/genetics, Membrane Cofactor Protein/metabolism, Membrane Glycoproteins/genetics, Receptors, Cell Surface/genetics, Viral Fusion Proteins/metabolism",
author = "{de Witte}, Lot and Marion Abt and Sibylle Schneider-Schaulies and {van Kooyk}, Yvette and Geijtenbeek, {Teunis B H}",
year = "2006",
month = "4",
doi = "10.1128/JVI.80.7.3477-3486.2006",
language = "English",
volume = "80",
pages = "3477--86",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "7",

}

Measles virus targets DC-SIGN to enhance dendritic cell infection. / de Witte, Lot; Abt, Marion; Schneider-Schaulies, Sibylle; van Kooyk, Yvette; Geijtenbeek, Teunis B H.

In: Journal of Virology, Vol. 80, No. 7, 04.2006, p. 3477-86.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Measles virus targets DC-SIGN to enhance dendritic cell infection

AU - de Witte, Lot

AU - Abt, Marion

AU - Schneider-Schaulies, Sibylle

AU - van Kooyk, Yvette

AU - Geijtenbeek, Teunis B H

PY - 2006/4

Y1 - 2006/4

N2 - Dendritic cells (DCs) are involved in the pathogenesis of measles virus (MV) infection by inducing immune suppression and possibly spreading the virus from the respiratory tract to lymphatic tissues. It is becoming evident that DC function can be modulated by the involvement of different receptors in pathogen interaction. Therefore, we have investigated the relative contributions of different MV-specific receptors on DCs to MV uptake into and infection of these cells. DCs express the MV receptors CD46 and CD150, and we demonstrate that the C-type lectin DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a novel receptor for laboratory-adapted and wild-type MV strains. The ligands for DC-SIGN are both MV glycoproteins F and H. In contrast to CD46 and CD150, DC-SIGN does not support MV entry, since DC-SIGN does not confer susceptibility when stably expressed in CHO cells. However, DC-SIGN is important for the infection of immature DCs with MV, since both attachment and infection of immature DCs with MV are blocked in the presence of DC-SIGN inhibitors. Our data demonstrate that DC-SIGN is crucial as an attachment receptor to enhance CD46/CD150-mediated infection of DCs in cis. Moreover, MV might not only target DC-SIGN to infect DCs but may also use DC-SIGN for viral transmission and immune suppression.

AB - Dendritic cells (DCs) are involved in the pathogenesis of measles virus (MV) infection by inducing immune suppression and possibly spreading the virus from the respiratory tract to lymphatic tissues. It is becoming evident that DC function can be modulated by the involvement of different receptors in pathogen interaction. Therefore, we have investigated the relative contributions of different MV-specific receptors on DCs to MV uptake into and infection of these cells. DCs express the MV receptors CD46 and CD150, and we demonstrate that the C-type lectin DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a novel receptor for laboratory-adapted and wild-type MV strains. The ligands for DC-SIGN are both MV glycoproteins F and H. In contrast to CD46 and CD150, DC-SIGN does not support MV entry, since DC-SIGN does not confer susceptibility when stably expressed in CHO cells. However, DC-SIGN is important for the infection of immature DCs with MV, since both attachment and infection of immature DCs with MV are blocked in the presence of DC-SIGN inhibitors. Our data demonstrate that DC-SIGN is crucial as an attachment receptor to enhance CD46/CD150-mediated infection of DCs in cis. Moreover, MV might not only target DC-SIGN to infect DCs but may also use DC-SIGN for viral transmission and immune suppression.

KW - Animals

KW - Antigens, CD/metabolism

KW - CHO Cells

KW - Cell Adhesion Molecules/genetics

KW - Cricetinae

KW - Dendritic Cells/cytology

KW - Enzyme-Linked Immunosorbent Assay

KW - Flow Cytometry

KW - Fluorescein-5-isothiocyanate

KW - Fluorescent Antibody Technique, Indirect

KW - Fluorescent Dyes

KW - Green Fluorescent Proteins/metabolism

KW - Humans

KW - K562 Cells

KW - Lectins, C-Type/genetics

KW - Ligands

KW - Measles/virology

KW - Measles virus/genetics

KW - Membrane Cofactor Protein/metabolism

KW - Membrane Glycoproteins/genetics

KW - Receptors, Cell Surface/genetics

KW - Viral Fusion Proteins/metabolism

U2 - 10.1128/JVI.80.7.3477-3486.2006

DO - 10.1128/JVI.80.7.3477-3486.2006

M3 - Article

VL - 80

SP - 3477

EP - 3486

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 7

ER -