Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid

Patricia M. Jones, Richard Boriack, Eduard A. Struys, Dinesh Rakheja

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

We describe a liquid chromatography-tandem mass spectrometry assay for measurement of d-2-hydroxyglutaric acid and l-2-hydroxyglutaric acid. These metabolites are increased in specific inborn errors of metabolism and are now recognized as oncometabolites. The measurement of d-2-hydroxyglutarate in peripheral blood may be used as a biomarker for screening and follow-up of patients with IDH-mutated acute myeloid leukemia.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages219-234
Number of pages16
Volume1633
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1633
ISSN (Print)1064-3745

Cite this

Jones, P. M., Boriack, R., Struys, E. A., & Rakheja, D. (2017). Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid. In Methods in Molecular Biology (Vol. 1633, pp. 219-234). (Methods in Molecular Biology; Vol. 1633). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7142-8_14
Jones, Patricia M. ; Boriack, Richard ; Struys, Eduard A. ; Rakheja, Dinesh. / Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid. Methods in Molecular Biology. Vol. 1633 Humana Press Inc., 2017. pp. 219-234 (Methods in Molecular Biology).
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abstract = "We describe a liquid chromatography-tandem mass spectrometry assay for measurement of d-2-hydroxyglutaric acid and l-2-hydroxyglutaric acid. These metabolites are increased in specific inborn errors of metabolism and are now recognized as oncometabolites. The measurement of d-2-hydroxyglutarate in peripheral blood may be used as a biomarker for screening and follow-up of patients with IDH-mutated acute myeloid leukemia.",
keywords = "2-Hydroxyglutaric acid, 2HG, Biomarker, D-2-Hydroxyglutaric acid, D-2HG, L-2-Hydroxyglutaric acid, L-2HG, LC-MS/MS, Liquid chromatography-tandem mass spectrometry, Oncometabolite",
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Jones, PM, Boriack, R, Struys, EA & Rakheja, D 2017, Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid. in Methods in Molecular Biology. vol. 1633, Methods in Molecular Biology, vol. 1633, Humana Press Inc., pp. 219-234. https://doi.org/10.1007/978-1-4939-7142-8_14

Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid. / Jones, Patricia M.; Boriack, Richard; Struys, Eduard A.; Rakheja, Dinesh.

Methods in Molecular Biology. Vol. 1633 Humana Press Inc., 2017. p. 219-234 (Methods in Molecular Biology; Vol. 1633).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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AB - We describe a liquid chromatography-tandem mass spectrometry assay for measurement of d-2-hydroxyglutaric acid and l-2-hydroxyglutaric acid. These metabolites are increased in specific inborn errors of metabolism and are now recognized as oncometabolites. The measurement of d-2-hydroxyglutarate in peripheral blood may be used as a biomarker for screening and follow-up of patients with IDH-mutated acute myeloid leukemia.

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KW - D-2-Hydroxyglutaric acid

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BT - Methods in Molecular Biology

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Jones PM, Boriack R, Struys EA, Rakheja D. Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid. In Methods in Molecular Biology. Vol. 1633. Humana Press Inc. 2017. p. 219-234. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7142-8_14