We describe a liquid chromatography-tandem mass spectrometry assay for measurement of d-2-hydroxyglutaric acid and l-2-hydroxyglutaric acid. These metabolites are increased in specific inborn errors of metabolism and are now recognized as oncometabolites. The measurement of d-2-hydroxyglutarate in peripheral blood may be used as a biomarker for screening and follow-up of patients with IDH-mutated acute myeloid leukemia.
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||16|
|Publication status||Published - 2017|
|Name||Methods in Molecular Biology|