Medium-chain, triglyceride-containing lipid emulsions increase human neutrophil beta2 integrin expression, adhesion, and degranulation

G J Wanten, T B Geijtenbeek, R A Raymakers, Y van Kooyk, D Roos, J B Jansen, A H Naber

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

BACKGROUND: To test the hypothesis that lipid emulsions with different triglyceride structures have distinct immunomodulatory properties, we analyzed human neutrophil adhesion and degranulation after lipid incubation.

METHODS: Neutrophils, isolated from the blood of 10 healthy volunteers, were incubated in medium or physiologic (2.5 mmol/L) emulsions containing long-chain (LCT), medium-chain (MCT), mixed LCT/MCT, or structured (SL) triglycerides. Expression of adhesion molecules and degranulation markers was evaluated by flow cytometry. Also, functional adhesion was investigated by means of a flow cytometric assay using fluorescent beads coated with the integrin ligand intercellular adhesion molecule (ICAM)-1.

RESULTS: Although LCT and SL had no effect, LCT/MCT significantly increased expression of the beta2 integrins lymphocyte-function-associated antigen 1 (+18%), macrophage antigen 1 (+387%), p150,95 (+82%), and (alphaDbeta2 (+230%). Degranulation marker expression for azurophilic (CD63, +210%) and specific granules (CD66b, +370%) also significantly increased, whereas L-selectin (CD62L, -70%) decreased. The effects of LCT/MCT were mimicked by the MCT emulsion. ICAM-1 adhesion (% beads bound) was increased by LCT/MCT (34% +/- 4%), whereas LCT (19% +/-3%) and SL (20% +/- 2%) had no effect compared with medium (17% +/- 3%).

CONCLUSIONS: LCT/MCT and MCT, contrary to LCT and SL emulsions, increased neutrophil beta2 integrin expression, adhesion, and degranulation. Apart from other emulsion constituents, triglyceride chain length might therefore be a key feature in the interaction of lipid emulsions and the phagocyte immune system.

Original languageEnglish
Pages (from-to)228-33
Number of pages6
JournalJournal of Parenteral and Enteral Nutition
Volume24
Issue number4
DOIs
Publication statusPublished - 8 Jul 2000

Cite this

@article{504caf5953f84316870ea3e0a6c677d7,
title = "Medium-chain, triglyceride-containing lipid emulsions increase human neutrophil beta2 integrin expression, adhesion, and degranulation",
abstract = "BACKGROUND: To test the hypothesis that lipid emulsions with different triglyceride structures have distinct immunomodulatory properties, we analyzed human neutrophil adhesion and degranulation after lipid incubation.METHODS: Neutrophils, isolated from the blood of 10 healthy volunteers, were incubated in medium or physiologic (2.5 mmol/L) emulsions containing long-chain (LCT), medium-chain (MCT), mixed LCT/MCT, or structured (SL) triglycerides. Expression of adhesion molecules and degranulation markers was evaluated by flow cytometry. Also, functional adhesion was investigated by means of a flow cytometric assay using fluorescent beads coated with the integrin ligand intercellular adhesion molecule (ICAM)-1.RESULTS: Although LCT and SL had no effect, LCT/MCT significantly increased expression of the beta2 integrins lymphocyte-function-associated antigen 1 (+18{\%}), macrophage antigen 1 (+387{\%}), p150,95 (+82{\%}), and (alphaDbeta2 (+230{\%}). Degranulation marker expression for azurophilic (CD63, +210{\%}) and specific granules (CD66b, +370{\%}) also significantly increased, whereas L-selectin (CD62L, -70{\%}) decreased. The effects of LCT/MCT were mimicked by the MCT emulsion. ICAM-1 adhesion ({\%} beads bound) was increased by LCT/MCT (34{\%} +/- 4{\%}), whereas LCT (19{\%} +/-3{\%}) and SL (20{\%} +/- 2{\%}) had no effect compared with medium (17{\%} +/- 3{\%}).CONCLUSIONS: LCT/MCT and MCT, contrary to LCT and SL emulsions, increased neutrophil beta2 integrin expression, adhesion, and degranulation. Apart from other emulsion constituents, triglyceride chain length might therefore be a key feature in the interaction of lipid emulsions and the phagocyte immune system.",
keywords = "Adult, CD18 Antigens/metabolism, Cell Adhesion/physiology, Cell Degranulation/physiology, Fat Emulsions, Intravenous/administration & dosage, Female, Flow Cytometry, Humans, Intercellular Adhesion Molecule-1/metabolism, Male, Middle Aged, Neutrophils/drug effects, Triglycerides/administration & dosage",
author = "Wanten, {G J} and Geijtenbeek, {T B} and Raymakers, {R A} and {van Kooyk}, Y and D Roos and Jansen, {J B} and Naber, {A H}",
year = "2000",
month = "7",
day = "8",
doi = "10.1177/0148607100024004228",
language = "English",
volume = "24",
pages = "228--33",
journal = "Journal of Parenteral and Enteral Nutition",
issn = "0148-6071",
publisher = "SAGE Publications Inc.",
number = "4",

}

Medium-chain, triglyceride-containing lipid emulsions increase human neutrophil beta2 integrin expression, adhesion, and degranulation. / Wanten, G J; Geijtenbeek, T B; Raymakers, R A; van Kooyk, Y; Roos, D; Jansen, J B; Naber, A H.

In: Journal of Parenteral and Enteral Nutition, Vol. 24, No. 4, 08.07.2000, p. 228-33.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Medium-chain, triglyceride-containing lipid emulsions increase human neutrophil beta2 integrin expression, adhesion, and degranulation

AU - Wanten, G J

AU - Geijtenbeek, T B

AU - Raymakers, R A

AU - van Kooyk, Y

AU - Roos, D

AU - Jansen, J B

AU - Naber, A H

PY - 2000/7/8

Y1 - 2000/7/8

N2 - BACKGROUND: To test the hypothesis that lipid emulsions with different triglyceride structures have distinct immunomodulatory properties, we analyzed human neutrophil adhesion and degranulation after lipid incubation.METHODS: Neutrophils, isolated from the blood of 10 healthy volunteers, were incubated in medium or physiologic (2.5 mmol/L) emulsions containing long-chain (LCT), medium-chain (MCT), mixed LCT/MCT, or structured (SL) triglycerides. Expression of adhesion molecules and degranulation markers was evaluated by flow cytometry. Also, functional adhesion was investigated by means of a flow cytometric assay using fluorescent beads coated with the integrin ligand intercellular adhesion molecule (ICAM)-1.RESULTS: Although LCT and SL had no effect, LCT/MCT significantly increased expression of the beta2 integrins lymphocyte-function-associated antigen 1 (+18%), macrophage antigen 1 (+387%), p150,95 (+82%), and (alphaDbeta2 (+230%). Degranulation marker expression for azurophilic (CD63, +210%) and specific granules (CD66b, +370%) also significantly increased, whereas L-selectin (CD62L, -70%) decreased. The effects of LCT/MCT were mimicked by the MCT emulsion. ICAM-1 adhesion (% beads bound) was increased by LCT/MCT (34% +/- 4%), whereas LCT (19% +/-3%) and SL (20% +/- 2%) had no effect compared with medium (17% +/- 3%).CONCLUSIONS: LCT/MCT and MCT, contrary to LCT and SL emulsions, increased neutrophil beta2 integrin expression, adhesion, and degranulation. Apart from other emulsion constituents, triglyceride chain length might therefore be a key feature in the interaction of lipid emulsions and the phagocyte immune system.

AB - BACKGROUND: To test the hypothesis that lipid emulsions with different triglyceride structures have distinct immunomodulatory properties, we analyzed human neutrophil adhesion and degranulation after lipid incubation.METHODS: Neutrophils, isolated from the blood of 10 healthy volunteers, were incubated in medium or physiologic (2.5 mmol/L) emulsions containing long-chain (LCT), medium-chain (MCT), mixed LCT/MCT, or structured (SL) triglycerides. Expression of adhesion molecules and degranulation markers was evaluated by flow cytometry. Also, functional adhesion was investigated by means of a flow cytometric assay using fluorescent beads coated with the integrin ligand intercellular adhesion molecule (ICAM)-1.RESULTS: Although LCT and SL had no effect, LCT/MCT significantly increased expression of the beta2 integrins lymphocyte-function-associated antigen 1 (+18%), macrophage antigen 1 (+387%), p150,95 (+82%), and (alphaDbeta2 (+230%). Degranulation marker expression for azurophilic (CD63, +210%) and specific granules (CD66b, +370%) also significantly increased, whereas L-selectin (CD62L, -70%) decreased. The effects of LCT/MCT were mimicked by the MCT emulsion. ICAM-1 adhesion (% beads bound) was increased by LCT/MCT (34% +/- 4%), whereas LCT (19% +/-3%) and SL (20% +/- 2%) had no effect compared with medium (17% +/- 3%).CONCLUSIONS: LCT/MCT and MCT, contrary to LCT and SL emulsions, increased neutrophil beta2 integrin expression, adhesion, and degranulation. Apart from other emulsion constituents, triglyceride chain length might therefore be a key feature in the interaction of lipid emulsions and the phagocyte immune system.

KW - Adult

KW - CD18 Antigens/metabolism

KW - Cell Adhesion/physiology

KW - Cell Degranulation/physiology

KW - Fat Emulsions, Intravenous/administration & dosage

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Intercellular Adhesion Molecule-1/metabolism

KW - Male

KW - Middle Aged

KW - Neutrophils/drug effects

KW - Triglycerides/administration & dosage

U2 - 10.1177/0148607100024004228

DO - 10.1177/0148607100024004228

M3 - Article

VL - 24

SP - 228

EP - 233

JO - Journal of Parenteral and Enteral Nutition

JF - Journal of Parenteral and Enteral Nutition

SN - 0148-6071

IS - 4

ER -