Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis

Maximilianos Kotsias, Athanasios Blanas, Sandra J. van Vliet, Martina Pirro, Daniel I. R. Spencer, Radoslaw P. Kozak

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated.
Original languageEnglish
Article numbere0223270
JournalPLoS ONE
Volume14
Issue number10
DOIs
Publication statusPublished - 1 Jan 2019

Cite this

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title = "Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis",
abstract = "The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated.",
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Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis. / Kotsias, Maximilianos; Blanas, Athanasios; van Vliet, Sandra J.; Pirro, Martina; Spencer, Daniel I. R.; Kozak, Radoslaw P.

In: PLoS ONE, Vol. 14, No. 10, e0223270, 01.01.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Kotsias, Maximilianos

AU - Blanas, Athanasios

AU - van Vliet, Sandra J.

AU - Pirro, Martina

AU - Spencer, Daniel I. R.

AU - Kozak, Radoslaw P.

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