MGL-mediated internalization and antigen presentation by dendritic cells: a role for tyrosine-5

Sandra J van Vliet, Corlien A Aarnoudse, Venice C M Broks-van den Berg, Martine Boks, Teunis B H Geijtenbeek, Yvette van Kooyk

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Professional antigen-presenting cells are essential for the initiation of adaptive immune responses; however, they also play a vital role in the maintenance of tolerance towards self-antigens. C-type lectins can function as antigen receptors by capturing carbohydrate ligands for processing and presentation. Here, we focused on the dendritic cell (DC)-expressed macrophage galactose-type lectin (MGL), a C-type lectin with a unique specificity for terminal GalNAc residues, such as the tumor-associated Tn antigen. Soluble model antigens are efficiently internalized by MGL and subsequently presented to responder CD4+ T cells. The tyrosine-5 residue in the YENF motif, present in the MGL cytoplasmic domain, was essential for the MGL-mediated endocytosis in CHO cells. In conclusion, MGL contributes to the antigen processing and presentation capacities of DC and may provide a suitable target for the initiation of anti-tumor immune responses.

Original languageEnglish
Pages (from-to)2075-81
Number of pages7
JournalEuropean Journal of Immunology
Issue number8
Publication statusPublished - Aug 2007

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