Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors

Rozita Jalali, Franck Guy, Samaneh Ghazanfari, Don Lyaruu, Leo van Ruijven, Pamela DenBesten, Stefania Martignon, Gina Castiblanco, Antonius L.J.J. Bronckers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Objective Fluoride excess of 0.05–0.07 mg F/kg bw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines. Design Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride. Result Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup–Fejerskov (TF) Index as III–IV showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (∼18%, p = 0.02) than in TF-I/II teeth. Conclusion The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.

Original languageEnglish
Pages (from-to)214-221
Number of pages8
JournalArchives of Oral Biology
Volume83
DOIs
Publication statusPublished - 1 Nov 2017

Cite this

Jalali, R., Guy, F., Ghazanfari, S., Lyaruu, D., van Ruijven, L., DenBesten, P., ... Bronckers, A. L. J. J. (2017). Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors. Archives of Oral Biology, 83, 214-221. https://doi.org/10.1016/j.archoralbio.2017.07.018
Jalali, Rozita ; Guy, Franck ; Ghazanfari, Samaneh ; Lyaruu, Don ; van Ruijven, Leo ; DenBesten, Pamela ; Martignon, Stefania ; Castiblanco, Gina ; Bronckers, Antonius L.J.J. / Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors. In: Archives of Oral Biology. 2017 ; Vol. 83. pp. 214-221.
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abstract = "Objective Fluoride excess of 0.05–0.07 mg F/kg bw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines. Design Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride. Result Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup–Fejerskov (TF) Index as III–IV showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (∼18{\%}, p = 0.02) than in TF-I/II teeth. Conclusion The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.",
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author = "Rozita Jalali and Franck Guy and Samaneh Ghazanfari and Don Lyaruu and {van Ruijven}, Leo and Pamela DenBesten and Stefania Martignon and Gina Castiblanco and Bronckers, {Antonius L.J.J.}",
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Jalali, R, Guy, F, Ghazanfari, S, Lyaruu, D, van Ruijven, L, DenBesten, P, Martignon, S, Castiblanco, G & Bronckers, ALJJ 2017, 'Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors' Archives of Oral Biology, vol. 83, pp. 214-221. https://doi.org/10.1016/j.archoralbio.2017.07.018

Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors. / Jalali, Rozita; Guy, Franck; Ghazanfari, Samaneh; Lyaruu, Don; van Ruijven, Leo; DenBesten, Pamela; Martignon, Stefania; Castiblanco, Gina; Bronckers, Antonius L.J.J.

In: Archives of Oral Biology, Vol. 83, 01.11.2017, p. 214-221.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors

AU - Jalali, Rozita

AU - Guy, Franck

AU - Ghazanfari, Samaneh

AU - Lyaruu, Don

AU - van Ruijven, Leo

AU - DenBesten, Pamela

AU - Martignon, Stefania

AU - Castiblanco, Gina

AU - Bronckers, Antonius L.J.J.

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Objective Fluoride excess of 0.05–0.07 mg F/kg bw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines. Design Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride. Result Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup–Fejerskov (TF) Index as III–IV showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (∼18%, p = 0.02) than in TF-I/II teeth. Conclusion The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.

AB - Objective Fluoride excess of 0.05–0.07 mg F/kg bw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines. Design Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride. Result Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup–Fejerskov (TF) Index as III–IV showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (∼18%, p = 0.02) than in TF-I/II teeth. Conclusion The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.

KW - Dental fluorosis

KW - Enamel development

KW - Hypermineralization

KW - Hypomineralization

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JF - Archives of Oral Biology

SN - 0003-9969

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