Modeling the quantitative nature of neurodevelopmental disorders using Collaborative Cross mice

Remco T. Molenhuis, Hilgo Bruining, Myrna J.V. Brandt, Petra E. Van Soldt, Hanifa J. Abu-Toamih Atamni, J. Peter H. Burbach, Fuad A. Iraqi, Richard F. Mott, Martien J.H. Kas*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: Animal models for neurodevelopmental disorders (NDD) generally rely on a single genetic mutation on a fixed genetic background. Recent human genetic studies however indicate that a clinical diagnosis with ASDAutism Spectrum Disorder (ASD) is almost always associated with multiple genetic fore- and background changes. The translational value of animal model studies would be greatly enhanced if genetic insults could be studied in a more quantitative framework across genetic backgrounds. Methods: We used the Collaborative Cross (CC), a novel mouse genetic reference population, to investigate the quantitative genetic architecture of mouse behavioral phenotypes commonly used in animal models for NDD. Results: Classical tests of social recognition and grooming phenotypes appeared insufficient for quantitative studies due to genetic dilution and limited heritability. In contrast, digging, locomotor activity, and stereotyped exploratory patterns were characterized by continuous distribution across our CC sample and also mapped to quantitative trait loci containing genes associated with corresponding phenotypes in human populations. Conclusions: These findings show that the CC can move animal model studies beyond comparative single gene-single background designs, and point out which type of behavioral phenotypes are most suitable to quantify the effect of developmental etiologies across multiple genetic backgrounds.

Original languageEnglish
Article number63
JournalMolecular Autism
Volume9
Issue number1
DOIs
Publication statusPublished - 13 Dec 2018
Externally publishedYes

Cite this

Molenhuis, R. T., Bruining, H., Brandt, M. J. V., Van Soldt, P. E., Abu-Toamih Atamni, H. J., Burbach, J. P. H., ... Kas, M. J. H. (2018). Modeling the quantitative nature of neurodevelopmental disorders using Collaborative Cross mice. Molecular Autism, 9(1), [63]. https://doi.org/10.1186/s13229-018-0252-2