Molecular cloning, expression and in vitro functional characterization of Myb-related proteins in Xenopus

T Bouwmeester, S Güehmann, T el-Baradi, F Kalkbrenner, I van Wijk, K Moelling, T Pieler

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Two cDNAs encoding Myb-related proteins have been cloned from Xenopus laevis and they have been termed Xmyb1 and Xmyb2. The Xmyb1 cDNA clone codes for an open reading frame of 733 amino acids and exhibits a high degree of similarity over the entire predicted protein sequence with the human B-Myb protein. Xmyb2 is a partial cDNA clone encoding three copies of amino-terminal tandem repeat elements typical for the Myb DNA-binding domain. The predicted protein sequence is most closely related to the human A-Myb gene product. In vitro translation of two deletion mutants of Xmyb1, truncated in the 3'-portion of the open reading frame, results in protein products which cross-react with polyvalent as well as monoclonal antibodies directed against the human c-Myb protein. The same two XMyb1 proteins, which both contain the complete set of aminoterminal repeats, specifically bind to the c-Myb-specific DNA binding sequence as evidenced by electrophoretic mobility shift analysis in vitro. RNA expression profiles of Xmyb1 and -2 are very different from each other; Xmyb1 is present throughout oogenesis and early Xenopus embryogenesis; in adult tissue it is primarily detected in blood. In contrast, Xmyb2 is expressed at only very low levels during oogenesis, not detectable in embryonic RNA preparations, and in adult tissue it is predominantly expressed in testis, with only a very low level seen in blood.

Original languageEnglish
Pages (from-to)57-68
Number of pages12
JournalMechanisms of development
Volume37
Issue number1-2
Publication statusPublished - Mar 1992

Cite this

Bouwmeester, T., Güehmann, S., el-Baradi, T., Kalkbrenner, F., van Wijk, I., Moelling, K., & Pieler, T. (1992). Molecular cloning, expression and in vitro functional characterization of Myb-related proteins in Xenopus. Mechanisms of development, 37(1-2), 57-68.