Molecular profiles of response to neoadjuvant chemoradiotherapy in oesophageal cancers to develop personalized treatment strategies

Leonie K. de Klerk, Ruben S. A. Goedegebuure, Nicole C. T. van Grieken, Johanna W. van Sandick, Annemieke Cats, Jurrien Stiekema, Rosa T. van der Kaaij, Arantza Farina Sarasqueta, Manon van Engeland, Maarten A. J. M. Jacobs, Roy L. J. van Wanrooij, Donald L. van der Peet, Aaron R. Thorner, Henk M. W. Verheul, Victor L. J. L. Thijssen, Adam J. Bass*, Sarah Derks*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Identification of molecular predictive markers of response to neoadjuvant chemoradiation could aid clinical decision-making in patients with localized oesophageal cancer. Therefore, we subjected pretreatment biopsies of 75 adenocarcinoma (OAC) and 16 squamous cell carcinoma (OSCC) patients to targeted next-generation DNA sequencing, as well as biopsies of 85 OAC and 20 OSCC patients to promoter methylation analysis of eight GI-specific genes, and subsequently searched for associations with histopathological response and disease-free (DFS) and overall survival (OS). Thereby, we found that in OAC, CSMD1 deletion (8%) and ETV4 amplification (5%) were associated with a favourable histopathological response, whereas SMURF1 amplification (5%) and SMARCA4 mutation (7%) were associated with an unfavourable histopathological response. KRAS (15%) and GATA4 (7%) amplification were associated with shorter OS. In OSCC, TP63 amplification (25%) and TFPI2 (10%) gene promoter methylation were associated with an unfavourable histopathological response and shorter DFS (TP63) and OS (TFPI2), whereas CDKN2A deletion (38%) was associated with prolonged OS. In conclusion, this study identified candidate genetic biomarkers associated with response to neoadjuvant chemoradiotherapy in patients with localized oesophageal cancer.
Original languageEnglish
Pages (from-to)901-914
Number of pages14
JournalMolecular oncology
Issue number4
Early online date2021
Publication statusPublished - Apr 2021

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