TY - JOUR
T1 - Molecular reactivity of mesocorticolimbic brain areas of high and low grooming rats after elevated plus maze exposure
AU - Koya, Eisuke
AU - Spijker, Sabine
AU - Homberg, Judith R
AU - Voorn, Pieter
AU - Schoffelmeer, Anton N M
AU - De Vries, Taco J
AU - Smit, August B.
PY - 2005/6/13
Y1 - 2005/6/13
N2 - High and low grooming rats (HG and LG), selected by extremities in stress-induced self-grooming on the elevated plus maze (EPM), display differences in stress coping style on the EPM, their motivation to self-administer cocaine, and differences in the reactivity of dopaminergic nerve terminals in mesocorticolimbic brain areas. This indicates a link between coping with a stressful/anxiogenic situation and drug intake. Here, we aimed to determine the molecular correlates of these differences by analyzing the reactivity of the mesocorticolimbic brain areas (the medial prefrontal cortex (mPFC) nucleus accumbens shell (NAS) and ventral tegmental area (VTA)) of HG and LG rats in response to EPM exposure. We report by measuring levels of immediate early gene (IEG) transcripts that EPM exposure-induced IEG expression was not significantly different between HG and LG rats. On the other hand, novel IEG expression patterns upon stress (EPM exposure) were apparent in all three areas including arc induction in the mPFC and NAS, CRH, BDNF, and Nr4a3 induction in the NAS, and serum glucocorticoid-regulated kinase (sgk) induction in the VTA. It is concluded that although the mPFC, NAS, and VTA play a role in modulating stress and grooming behavior, the neuronal reactivity in these regions measured by the IEG response is not related with behavioral extremities in stress coping style displayed on the EPM.
AB - High and low grooming rats (HG and LG), selected by extremities in stress-induced self-grooming on the elevated plus maze (EPM), display differences in stress coping style on the EPM, their motivation to self-administer cocaine, and differences in the reactivity of dopaminergic nerve terminals in mesocorticolimbic brain areas. This indicates a link between coping with a stressful/anxiogenic situation and drug intake. Here, we aimed to determine the molecular correlates of these differences by analyzing the reactivity of the mesocorticolimbic brain areas (the medial prefrontal cortex (mPFC) nucleus accumbens shell (NAS) and ventral tegmental area (VTA)) of HG and LG rats in response to EPM exposure. We report by measuring levels of immediate early gene (IEG) transcripts that EPM exposure-induced IEG expression was not significantly different between HG and LG rats. On the other hand, novel IEG expression patterns upon stress (EPM exposure) were apparent in all three areas including arc induction in the mPFC and NAS, CRH, BDNF, and Nr4a3 induction in the NAS, and serum glucocorticoid-regulated kinase (sgk) induction in the VTA. It is concluded that although the mPFC, NAS, and VTA play a role in modulating stress and grooming behavior, the neuronal reactivity in these regions measured by the IEG response is not related with behavioral extremities in stress coping style displayed on the EPM.
KW - Adaptation, Psychological
KW - Animals
KW - Anxiety Disorders
KW - Brain-Derived Neurotrophic Factor
KW - Corticotropin-Releasing Hormone
KW - Cytoskeletal Proteins
KW - DNA-Binding Proteins
KW - Early Growth Response Protein 1
KW - Early Growth Response Protein 2
KW - Genes, Immediate-Early
KW - Grooming
KW - Immediate-Early Proteins
KW - Limbic System
KW - Male
KW - Maze Learning
KW - Nerve Tissue Proteins
KW - Neural Pathways
KW - Nuclear Proteins
KW - Nucleus Accumbens
KW - Prefrontal Cortex
KW - Protein-Serine-Threonine Kinases
KW - RNA, Messenger
KW - Rats
KW - Rats, Wistar
KW - Stress, Psychological
KW - Transcription Factors
KW - Ventral Tegmental Area
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.molbrainres.2005.03.005
DO - 10.1016/j.molbrainres.2005.03.005
M3 - Article
C2 - 15950777
VL - 137
SP - 184
EP - 192
JO - Experimental Brain Research
JF - Experimental Brain Research
SN - 0014-4819
IS - 1-2
ER -