Mouse adenovirus type 1 infection of macrophages

S.L. Ashley, A.R. Welton, K.M. Harwood, N. van Rooijen, K.R. Spindler

Research output: Contribution to journalArticleAcademicpeer-review


Mouse adenovirus type 1 (MAV-1) causes acute and persistent infections in mice, with high levels of virus found in the brain, spinal cord and spleen in acute infections. MAV-1 infects endothelial cells throughout the mouse, and monocytes/macrophages have also been implicated as targets of the virus. Here we determined the extent and functional importance of macrophage infection by MAV-1. Bone marrow-derived macrophages expressed MAV-1 mRNAs and proteins upon ex vivo infection. Adherent peritoneal macrophages from infected mice expressed viral mRNAs and produced infectious virus. Infected chemokine (C-C motif) receptor 2 (CCR2) knockout mice, which are defective for macrophage recruitment, did not show differences in survival or MAV-1 load compared to controls. In contrast, macrophage depletion using clodronate-loaded liposomes resulted in increased virus replication in spleens of a MAV-1-resistant mouse strain, BALB/cJ. Thus macrophages serve both as targets of infection and as effectors of the host response. (C) 2009 Elsevier Inc. All rights reserved
Original languageUndefined/Unknown
Pages (from-to)307-314
Issue number2
Publication statusPublished - 2009

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