Multi-omics profile of the mouse dentate gyrus after kainic acid-induced status epilepticus

Marijn Schouten, Pascal Bielefeld, Silvina A Fratantoni, Chantal J Hubens, Sander R Piersma, Thang V Pham, Rob A Voskuyl, Paul J Lucassen, Connie R Jimenez, Carlos P Fitzsimons

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Temporal lobe epilepsy (TLE) can develop from alterations in hippocampal structure and circuit characteristics, and can be modeled in mice by administration of kainic acid (KA). Adult neurogenesis in the dentate gyrus (DG) contributes to hippocampal functions and has been reported to contribute to the development of TLE. Some of the phenotypical changes include neural stem and precursor cells (NPSC) apoptosis, shortly after their birth, before they produce hippocampal neurons. Here we explored these early phenotypical changes in the DG 3 days after a systemic injection of KA inducing status epilepticus (KA-SE), in mice. We performed a multi-omics experimental setup and analyzed DG tissue samples using proteomics, transcriptomics and microRNA profiling techniques, detecting the expression of 2327 proteins, 13401 mRNAs and 311 microRNAs. We here present a description of how these data were obtained and make them available for further analysis and validation. Our data may help to further identify and characterize molecular mechanisms involved in the alterations induced shortly after KA-SE in the mouse DG.

Original languageEnglish
Pages (from-to)160068
JournalScientific data
Volume3
DOIs
Publication statusPublished - 16 Aug 2016

Cite this

Schouten, Marijn ; Bielefeld, Pascal ; Fratantoni, Silvina A ; Hubens, Chantal J ; Piersma, Sander R ; Pham, Thang V ; Voskuyl, Rob A ; Lucassen, Paul J ; Jimenez, Connie R ; Fitzsimons, Carlos P. / Multi-omics profile of the mouse dentate gyrus after kainic acid-induced status epilepticus. In: Scientific data. 2016 ; Vol. 3. pp. 160068.
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abstract = "Temporal lobe epilepsy (TLE) can develop from alterations in hippocampal structure and circuit characteristics, and can be modeled in mice by administration of kainic acid (KA). Adult neurogenesis in the dentate gyrus (DG) contributes to hippocampal functions and has been reported to contribute to the development of TLE. Some of the phenotypical changes include neural stem and precursor cells (NPSC) apoptosis, shortly after their birth, before they produce hippocampal neurons. Here we explored these early phenotypical changes in the DG 3 days after a systemic injection of KA inducing status epilepticus (KA-SE), in mice. We performed a multi-omics experimental setup and analyzed DG tissue samples using proteomics, transcriptomics and microRNA profiling techniques, detecting the expression of 2327 proteins, 13401 mRNAs and 311 microRNAs. We here present a description of how these data were obtained and make them available for further analysis and validation. Our data may help to further identify and characterize molecular mechanisms involved in the alterations induced shortly after KA-SE in the mouse DG.",
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author = "Marijn Schouten and Pascal Bielefeld and Fratantoni, {Silvina A} and Hubens, {Chantal J} and Piersma, {Sander R} and Pham, {Thang V} and Voskuyl, {Rob A} and Lucassen, {Paul J} and Jimenez, {Connie R} and Fitzsimons, {Carlos P}",
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Schouten, M, Bielefeld, P, Fratantoni, SA, Hubens, CJ, Piersma, SR, Pham, TV, Voskuyl, RA, Lucassen, PJ, Jimenez, CR & Fitzsimons, CP 2016, 'Multi-omics profile of the mouse dentate gyrus after kainic acid-induced status epilepticus' Scientific data, vol. 3, pp. 160068. https://doi.org/10.1038/sdata.2016.68

Multi-omics profile of the mouse dentate gyrus after kainic acid-induced status epilepticus. / Schouten, Marijn; Bielefeld, Pascal; Fratantoni, Silvina A; Hubens, Chantal J; Piersma, Sander R; Pham, Thang V; Voskuyl, Rob A; Lucassen, Paul J; Jimenez, Connie R; Fitzsimons, Carlos P.

In: Scientific data, Vol. 3, 16.08.2016, p. 160068.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Schouten, Marijn

AU - Bielefeld, Pascal

AU - Fratantoni, Silvina A

AU - Hubens, Chantal J

AU - Piersma, Sander R

AU - Pham, Thang V

AU - Voskuyl, Rob A

AU - Lucassen, Paul J

AU - Jimenez, Connie R

AU - Fitzsimons, Carlos P

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N2 - Temporal lobe epilepsy (TLE) can develop from alterations in hippocampal structure and circuit characteristics, and can be modeled in mice by administration of kainic acid (KA). Adult neurogenesis in the dentate gyrus (DG) contributes to hippocampal functions and has been reported to contribute to the development of TLE. Some of the phenotypical changes include neural stem and precursor cells (NPSC) apoptosis, shortly after their birth, before they produce hippocampal neurons. Here we explored these early phenotypical changes in the DG 3 days after a systemic injection of KA inducing status epilepticus (KA-SE), in mice. We performed a multi-omics experimental setup and analyzed DG tissue samples using proteomics, transcriptomics and microRNA profiling techniques, detecting the expression of 2327 proteins, 13401 mRNAs and 311 microRNAs. We here present a description of how these data were obtained and make them available for further analysis and validation. Our data may help to further identify and characterize molecular mechanisms involved in the alterations induced shortly after KA-SE in the mouse DG.

AB - Temporal lobe epilepsy (TLE) can develop from alterations in hippocampal structure and circuit characteristics, and can be modeled in mice by administration of kainic acid (KA). Adult neurogenesis in the dentate gyrus (DG) contributes to hippocampal functions and has been reported to contribute to the development of TLE. Some of the phenotypical changes include neural stem and precursor cells (NPSC) apoptosis, shortly after their birth, before they produce hippocampal neurons. Here we explored these early phenotypical changes in the DG 3 days after a systemic injection of KA inducing status epilepticus (KA-SE), in mice. We performed a multi-omics experimental setup and analyzed DG tissue samples using proteomics, transcriptomics and microRNA profiling techniques, detecting the expression of 2327 proteins, 13401 mRNAs and 311 microRNAs. We here present a description of how these data were obtained and make them available for further analysis and validation. Our data may help to further identify and characterize molecular mechanisms involved in the alterations induced shortly after KA-SE in the mouse DG.

KW - Animals

KW - Dentate Gyrus/drug effects

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KW - Hippocampus/drug effects

KW - Kainic Acid

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KW - Status Epilepticus/chemically induced

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M3 - Article

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SP - 160068

JO - Scientific data

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SN - 2052-4463

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