MUTZ-3, a human cell line model for the cytokine-induced differentiation of dendritic cells from CD34+ precursors

Allan J Masterson, Claudia C Sombroek, Tanja D De Gruijl, Yvo M F Graus, Hans J J van der Vliet, Sinéad M Lougheed, Alfons J M van den Eertwegh, Herbert M Pinedo, Rik J Scheper

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Many human myeloid leukemia-derived cell lines possess the ability to acquire a dendritic cell (DC) phenotype. However, cytokine responsiveness is generally poor, requiring direct manipulation of intracellular signaling mechanisms for differentiation. In contrast, the CD34+ human acute myeloid leukemia cell line MUTZ-3 responds to granulocyte macrophage- colony-stimulating factor (GM-CSF), interleukin 4 (IL-4), and tumor necrosis factor alpha (TNFalpha), cytokines known to be pivotal both in vivo and in vitro for DC generation from monocytes and CD34+ stem cells. In all respects, MUTZ-3 cells behave as the immortalized equivalent of CD34+ DC precursors. Upon stimulation with specific cytokine cocktails, they acquire a phenotype consistent with either interstitial- or Langerhans-like DCs and upon maturation (mDC), express CD83. MUTZ-3 DC display the full range of functional antigen processing and presentation pathways. These findings demonstrate the unique suitability of MUTZ-3 cells as an unlimited source of CD34+ DC progenitors for the study of cytokine-induced DC differentiation.

Original languageEnglish
Pages (from-to)701-3
Number of pages3
Issue number2
Publication statusPublished - 15 Jul 2002

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