Myofibrillar and Mitochondrial Protein Synthesis Rates Do Not Differ in Young Men Following the Ingestion of Carbohydrate with Whey, Soy, or Leucine-Enriched Soy Protein after Concurrent Resistance- and Endurance-Type Exercise

Tyler A. Churchward-Venne, Philippe J. M. Pinckaers, Joey S. J. Smeets, Wouter M. Peeters, Antoine H. Zorenc, Henk Schierbeek, Ian Rollo, Lex B. Verdijk, Luc J. C. van Loon

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Whey and micellar casein are high-quality dairy proteins that can stimulate postprandial muscle protein synthesis rates. How whey and casein compare with milk protein in their capacity to stimulate postprandial myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis rates during postexercise recovery is currently unknown. Objective: The objective of this study was to compare postprandial MyoPS and MitoPS rates after protein-carbohydrate co-ingestion with milk protein, whey, or micellar casein during recovery from a single bout of concurrent resistance- and endurance-type exercise in young healthy men. Methods: In a randomized, double-blind, parallel-group design, 48 healthy, young, recreationally active men (mean SEM age: 23 0.3 y) received a primed continuous infusion of L-[ring-13C6]-phenylalanine and L-[ring-3,5-2H2]-tyrosine and ingested 45 g carbohydrate with 0 g protein (CHO), 20 g milk protein (MILK), 20 g whey protein (WHEY), or 20 g micellar casein protein (CASEIN) after a sequential bout of resistance- and endurance-type exercise (i.e., concurrent exercise). Blood and muscle biopsies were collected over 360 min during recovery from exercise to assess MyoPS and MitoPS rates and signaling through mammalian target of rapamycin complex 1 (mTORC1). Results: Despite temporal differences in postprandial plasma leucine concentrations between treatments (P < 0.001), MyoPS rates over 360 min of recovery did not differ between treatments (CHO: 0.049% 0.003%/h; MILK: 0.059% 0.003%/h; WHEY: 0.054% 0.002%/h; CASEIN: 0.059% 0.005%/h; P = 0.11). When MILK, WHEY, and CASEIN were pooled into a single group (PROTEIN), protein co-ingestion resulted in greater MyoPS rates compared with CHO (PROTEIN: 0.057% 0.002%/h; CHO: 0.049% 0.003%/h; P = 0.04). MitoPS rates and signaling through the mTORC1 pathway were similar between treatments. Conclusion: MyoPS and MitoPS rates do not differ after co-ingestion of either milk protein, whey protein, or micellar casein protein with carbohydrate during recovery from a single bout of concurrent resistance- and endurance-type exercise in recreationally active young men. Co-ingestion of protein with carbohydrate results in greater MyoPS, but not MitoPS rates, when compared with the ingestion of carbohydrate only during recovery from concurrent exercise. This trial was registered at Nederlands Trial Register: NTR5098. J Nutr 2019;149:198-209.
Original languageEnglish
Pages (from-to)210-220
JournalJournal of Nutrition
Volume149
Issue number2
DOIs
Publication statusPublished - 1 Feb 2019
Externally publishedYes

Cite this

Churchward-Venne, Tyler A. ; Pinckaers, Philippe J. M. ; Smeets, Joey S. J. ; Peeters, Wouter M. ; Zorenc, Antoine H. ; Schierbeek, Henk ; Rollo, Ian ; Verdijk, Lex B. ; van Loon, Luc J. C. / Myofibrillar and Mitochondrial Protein Synthesis Rates Do Not Differ in Young Men Following the Ingestion of Carbohydrate with Whey, Soy, or Leucine-Enriched Soy Protein after Concurrent Resistance- and Endurance-Type Exercise. In: Journal of Nutrition. 2019 ; Vol. 149, No. 2. pp. 210-220.
@article{0b0512a1919a4800a9455d4769654362,
title = "Myofibrillar and Mitochondrial Protein Synthesis Rates Do Not Differ in Young Men Following the Ingestion of Carbohydrate with Whey, Soy, or Leucine-Enriched Soy Protein after Concurrent Resistance- and Endurance-Type Exercise",
abstract = "Whey and micellar casein are high-quality dairy proteins that can stimulate postprandial muscle protein synthesis rates. How whey and casein compare with milk protein in their capacity to stimulate postprandial myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis rates during postexercise recovery is currently unknown. Objective: The objective of this study was to compare postprandial MyoPS and MitoPS rates after protein-carbohydrate co-ingestion with milk protein, whey, or micellar casein during recovery from a single bout of concurrent resistance- and endurance-type exercise in young healthy men. Methods: In a randomized, double-blind, parallel-group design, 48 healthy, young, recreationally active men (mean SEM age: 23 0.3 y) received a primed continuous infusion of L-[ring-13C6]-phenylalanine and L-[ring-3,5-2H2]-tyrosine and ingested 45 g carbohydrate with 0 g protein (CHO), 20 g milk protein (MILK), 20 g whey protein (WHEY), or 20 g micellar casein protein (CASEIN) after a sequential bout of resistance- and endurance-type exercise (i.e., concurrent exercise). Blood and muscle biopsies were collected over 360 min during recovery from exercise to assess MyoPS and MitoPS rates and signaling through mammalian target of rapamycin complex 1 (mTORC1). Results: Despite temporal differences in postprandial plasma leucine concentrations between treatments (P < 0.001), MyoPS rates over 360 min of recovery did not differ between treatments (CHO: 0.049{\%} 0.003{\%}/h; MILK: 0.059{\%} 0.003{\%}/h; WHEY: 0.054{\%} 0.002{\%}/h; CASEIN: 0.059{\%} 0.005{\%}/h; P = 0.11). When MILK, WHEY, and CASEIN were pooled into a single group (PROTEIN), protein co-ingestion resulted in greater MyoPS rates compared with CHO (PROTEIN: 0.057{\%} 0.002{\%}/h; CHO: 0.049{\%} 0.003{\%}/h; P = 0.04). MitoPS rates and signaling through the mTORC1 pathway were similar between treatments. Conclusion: MyoPS and MitoPS rates do not differ after co-ingestion of either milk protein, whey protein, or micellar casein protein with carbohydrate during recovery from a single bout of concurrent resistance- and endurance-type exercise in recreationally active young men. Co-ingestion of protein with carbohydrate results in greater MyoPS, but not MitoPS rates, when compared with the ingestion of carbohydrate only during recovery from concurrent exercise. This trial was registered at Nederlands Trial Register: NTR5098. J Nutr 2019;149:198-209.",
author = "Churchward-Venne, {Tyler A.} and Pinckaers, {Philippe J. M.} and Smeets, {Joey S. J.} and Peeters, {Wouter M.} and Zorenc, {Antoine H.} and Henk Schierbeek and Ian Rollo and Verdijk, {Lex B.} and {van Loon}, {Luc J. C.}",
year = "2019",
month = "2",
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doi = "10.1093/jn/nxy251",
language = "English",
volume = "149",
pages = "210--220",
journal = "The Journal of nutrition",
issn = "0022-3166",
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Myofibrillar and Mitochondrial Protein Synthesis Rates Do Not Differ in Young Men Following the Ingestion of Carbohydrate with Whey, Soy, or Leucine-Enriched Soy Protein after Concurrent Resistance- and Endurance-Type Exercise. / Churchward-Venne, Tyler A.; Pinckaers, Philippe J. M.; Smeets, Joey S. J.; Peeters, Wouter M.; Zorenc, Antoine H.; Schierbeek, Henk; Rollo, Ian; Verdijk, Lex B.; van Loon, Luc J. C.

In: Journal of Nutrition, Vol. 149, No. 2, 01.02.2019, p. 210-220.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Myofibrillar and Mitochondrial Protein Synthesis Rates Do Not Differ in Young Men Following the Ingestion of Carbohydrate with Whey, Soy, or Leucine-Enriched Soy Protein after Concurrent Resistance- and Endurance-Type Exercise

AU - Churchward-Venne, Tyler A.

AU - Pinckaers, Philippe J. M.

AU - Smeets, Joey S. J.

AU - Peeters, Wouter M.

AU - Zorenc, Antoine H.

AU - Schierbeek, Henk

AU - Rollo, Ian

AU - Verdijk, Lex B.

AU - van Loon, Luc J. C.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Whey and micellar casein are high-quality dairy proteins that can stimulate postprandial muscle protein synthesis rates. How whey and casein compare with milk protein in their capacity to stimulate postprandial myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis rates during postexercise recovery is currently unknown. Objective: The objective of this study was to compare postprandial MyoPS and MitoPS rates after protein-carbohydrate co-ingestion with milk protein, whey, or micellar casein during recovery from a single bout of concurrent resistance- and endurance-type exercise in young healthy men. Methods: In a randomized, double-blind, parallel-group design, 48 healthy, young, recreationally active men (mean SEM age: 23 0.3 y) received a primed continuous infusion of L-[ring-13C6]-phenylalanine and L-[ring-3,5-2H2]-tyrosine and ingested 45 g carbohydrate with 0 g protein (CHO), 20 g milk protein (MILK), 20 g whey protein (WHEY), or 20 g micellar casein protein (CASEIN) after a sequential bout of resistance- and endurance-type exercise (i.e., concurrent exercise). Blood and muscle biopsies were collected over 360 min during recovery from exercise to assess MyoPS and MitoPS rates and signaling through mammalian target of rapamycin complex 1 (mTORC1). Results: Despite temporal differences in postprandial plasma leucine concentrations between treatments (P < 0.001), MyoPS rates over 360 min of recovery did not differ between treatments (CHO: 0.049% 0.003%/h; MILK: 0.059% 0.003%/h; WHEY: 0.054% 0.002%/h; CASEIN: 0.059% 0.005%/h; P = 0.11). When MILK, WHEY, and CASEIN were pooled into a single group (PROTEIN), protein co-ingestion resulted in greater MyoPS rates compared with CHO (PROTEIN: 0.057% 0.002%/h; CHO: 0.049% 0.003%/h; P = 0.04). MitoPS rates and signaling through the mTORC1 pathway were similar between treatments. Conclusion: MyoPS and MitoPS rates do not differ after co-ingestion of either milk protein, whey protein, or micellar casein protein with carbohydrate during recovery from a single bout of concurrent resistance- and endurance-type exercise in recreationally active young men. Co-ingestion of protein with carbohydrate results in greater MyoPS, but not MitoPS rates, when compared with the ingestion of carbohydrate only during recovery from concurrent exercise. This trial was registered at Nederlands Trial Register: NTR5098. J Nutr 2019;149:198-209.

AB - Whey and micellar casein are high-quality dairy proteins that can stimulate postprandial muscle protein synthesis rates. How whey and casein compare with milk protein in their capacity to stimulate postprandial myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis rates during postexercise recovery is currently unknown. Objective: The objective of this study was to compare postprandial MyoPS and MitoPS rates after protein-carbohydrate co-ingestion with milk protein, whey, or micellar casein during recovery from a single bout of concurrent resistance- and endurance-type exercise in young healthy men. Methods: In a randomized, double-blind, parallel-group design, 48 healthy, young, recreationally active men (mean SEM age: 23 0.3 y) received a primed continuous infusion of L-[ring-13C6]-phenylalanine and L-[ring-3,5-2H2]-tyrosine and ingested 45 g carbohydrate with 0 g protein (CHO), 20 g milk protein (MILK), 20 g whey protein (WHEY), or 20 g micellar casein protein (CASEIN) after a sequential bout of resistance- and endurance-type exercise (i.e., concurrent exercise). Blood and muscle biopsies were collected over 360 min during recovery from exercise to assess MyoPS and MitoPS rates and signaling through mammalian target of rapamycin complex 1 (mTORC1). Results: Despite temporal differences in postprandial plasma leucine concentrations between treatments (P < 0.001), MyoPS rates over 360 min of recovery did not differ between treatments (CHO: 0.049% 0.003%/h; MILK: 0.059% 0.003%/h; WHEY: 0.054% 0.002%/h; CASEIN: 0.059% 0.005%/h; P = 0.11). When MILK, WHEY, and CASEIN were pooled into a single group (PROTEIN), protein co-ingestion resulted in greater MyoPS rates compared with CHO (PROTEIN: 0.057% 0.002%/h; CHO: 0.049% 0.003%/h; P = 0.04). MitoPS rates and signaling through the mTORC1 pathway were similar between treatments. Conclusion: MyoPS and MitoPS rates do not differ after co-ingestion of either milk protein, whey protein, or micellar casein protein with carbohydrate during recovery from a single bout of concurrent resistance- and endurance-type exercise in recreationally active young men. Co-ingestion of protein with carbohydrate results in greater MyoPS, but not MitoPS rates, when compared with the ingestion of carbohydrate only during recovery from concurrent exercise. This trial was registered at Nederlands Trial Register: NTR5098. J Nutr 2019;149:198-209.

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