TY - JOUR
T1 - Narrowband-auto fluorescence imaging for bone analysis
AU - Fauch, Laure
AU - Palander, Anni
AU - Dekker, Hannah
AU - Schulten, Engelbert A.J.M.
AU - Koistinen, Arto
AU - Kullaa, Arja
AU - Keinänen, Markku
PY - 2019/5/1
Y1 - 2019/5/1
N2 - We present a new autofluorescence-imaging method for bone analysis. This method, based on the autofluorescence of bone, provides color images in microscopic scale. The color images are created from three monochrome images acquired with optimal excitation- and emission-wavelengths combinations. The choice of these combinations were determined from the study of two-dimensional distributions of bone-features-bispectral autofluorescence in the visible- and ultraviolet-spectral range. We demonstrate that main-bone features visualized with MG-staining method can also be visualized in the autofluorescence-color image. Furthermore, the autofluorescence-color image presents features hardly distinguished in a histological-bone section.
AB - We present a new autofluorescence-imaging method for bone analysis. This method, based on the autofluorescence of bone, provides color images in microscopic scale. The color images are created from three monochrome images acquired with optimal excitation- and emission-wavelengths combinations. The choice of these combinations were determined from the study of two-dimensional distributions of bone-features-bispectral autofluorescence in the visible- and ultraviolet-spectral range. We demonstrate that main-bone features visualized with MG-staining method can also be visualized in the autofluorescence-color image. Furthermore, the autofluorescence-color image presents features hardly distinguished in a histological-bone section.
UR - http://www.scopus.com/inward/record.url?scp=85078722011&partnerID=8YFLogxK
U2 - 10.1364/BOE.10.002367
DO - 10.1364/BOE.10.002367
M3 - Article
C2 - 31149377
AN - SCOPUS:85078722011
SN - 2156-7085
VL - 10
SP - 2367
EP - 2382
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 5
ER -