Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function

Liana Steeghs, Sandra J van Vliet, Heli Uronen-Hansson, Andries van Mourik, Anneke Engering, Martha Sanchez-Hernandez, Nigel Klein, Robin Callard, Jos P M van Putten, Peter van der Ley, Yvette van Kooyk, Jan G J van de Winkel

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Neisseria meningitidis lipopolysaccharide (LPS) has been identified as a major determinant of dendritic cell (DC) function. Here we report that one of a series of meningococcal mutants with defined truncations in the lacto-N-neotetraose outer core of the LPS exhibited unique strong adhesion and internalization properties towards DC. These properties were mediated by interaction of the GlcNAc(beta1-3)-Gal(beta1-4)-Glc-R oligosaccharide outer core of lgtB LPS with the dendritic-cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) lectin receptor. Activation of DC-SIGN with this novel oligosaccharide ligand skewed T-cell responses driven by DC towards T helper type 1 activity. Thus, the use of lgtB LPS may provide a powerful instrument to selectively induce the desired arm of the immune response and potentially increase vaccine efficacy.

Original languageEnglish
Pages (from-to)316-25
Number of pages10
JournalCellular Microbiology
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 2006

Cite this

Steeghs, L., van Vliet, S. J., Uronen-Hansson, H., van Mourik, A., Engering, A., Sanchez-Hernandez, M., ... van de Winkel, J. G. J. (2006). Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function. Cellular Microbiology, 8(2), 316-25. https://doi.org/10.1111/j.1462-5822.2005.00623.x
Steeghs, Liana ; van Vliet, Sandra J ; Uronen-Hansson, Heli ; van Mourik, Andries ; Engering, Anneke ; Sanchez-Hernandez, Martha ; Klein, Nigel ; Callard, Robin ; van Putten, Jos P M ; van der Ley, Peter ; van Kooyk, Yvette ; van de Winkel, Jan G J. / Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function. In: Cellular Microbiology. 2006 ; Vol. 8, No. 2. pp. 316-25.
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title = "Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function",
abstract = "Neisseria meningitidis lipopolysaccharide (LPS) has been identified as a major determinant of dendritic cell (DC) function. Here we report that one of a series of meningococcal mutants with defined truncations in the lacto-N-neotetraose outer core of the LPS exhibited unique strong adhesion and internalization properties towards DC. These properties were mediated by interaction of the GlcNAc(beta1-3)-Gal(beta1-4)-Glc-R oligosaccharide outer core of lgtB LPS with the dendritic-cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) lectin receptor. Activation of DC-SIGN with this novel oligosaccharide ligand skewed T-cell responses driven by DC towards T helper type 1 activity. Thus, the use of lgtB LPS may provide a powerful instrument to selectively induce the desired arm of the immune response and potentially increase vaccine efficacy.",
keywords = "Antigen Presentation, Bacterial Adhesion, Cell Adhesion Molecules/metabolism, Cell Differentiation, Cells, Cultured, Cytokines/biosynthesis, Dendritic Cells/immunology, Humans, Lectins, C-Type/metabolism, Lipopolysaccharides/metabolism, Mutation, Neisseria meningitidis/genetics, Oligosaccharides/genetics, Receptors, Cell Surface/metabolism, Th1 Cells/cytology, Th2 Cells/cytology",
author = "Liana Steeghs and {van Vliet}, {Sandra J} and Heli Uronen-Hansson and {van Mourik}, Andries and Anneke Engering and Martha Sanchez-Hernandez and Nigel Klein and Robin Callard and {van Putten}, {Jos P M} and {van der Ley}, Peter and {van Kooyk}, Yvette and {van de Winkel}, {Jan G J}",
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Steeghs, L, van Vliet, SJ, Uronen-Hansson, H, van Mourik, A, Engering, A, Sanchez-Hernandez, M, Klein, N, Callard, R, van Putten, JPM, van der Ley, P, van Kooyk, Y & van de Winkel, JGJ 2006, 'Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function' Cellular Microbiology, vol. 8, no. 2, pp. 316-25. https://doi.org/10.1111/j.1462-5822.2005.00623.x

Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function. / Steeghs, Liana; van Vliet, Sandra J; Uronen-Hansson, Heli; van Mourik, Andries; Engering, Anneke; Sanchez-Hernandez, Martha; Klein, Nigel; Callard, Robin; van Putten, Jos P M; van der Ley, Peter; van Kooyk, Yvette; van de Winkel, Jan G J.

In: Cellular Microbiology, Vol. 8, No. 2, 02.2006, p. 316-25.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van Vliet, Sandra J

AU - Uronen-Hansson, Heli

AU - van Mourik, Andries

AU - Engering, Anneke

AU - Sanchez-Hernandez, Martha

AU - Klein, Nigel

AU - Callard, Robin

AU - van Putten, Jos P M

AU - van der Ley, Peter

AU - van Kooyk, Yvette

AU - van de Winkel, Jan G J

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N2 - Neisseria meningitidis lipopolysaccharide (LPS) has been identified as a major determinant of dendritic cell (DC) function. Here we report that one of a series of meningococcal mutants with defined truncations in the lacto-N-neotetraose outer core of the LPS exhibited unique strong adhesion and internalization properties towards DC. These properties were mediated by interaction of the GlcNAc(beta1-3)-Gal(beta1-4)-Glc-R oligosaccharide outer core of lgtB LPS with the dendritic-cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) lectin receptor. Activation of DC-SIGN with this novel oligosaccharide ligand skewed T-cell responses driven by DC towards T helper type 1 activity. Thus, the use of lgtB LPS may provide a powerful instrument to selectively induce the desired arm of the immune response and potentially increase vaccine efficacy.

AB - Neisseria meningitidis lipopolysaccharide (LPS) has been identified as a major determinant of dendritic cell (DC) function. Here we report that one of a series of meningococcal mutants with defined truncations in the lacto-N-neotetraose outer core of the LPS exhibited unique strong adhesion and internalization properties towards DC. These properties were mediated by interaction of the GlcNAc(beta1-3)-Gal(beta1-4)-Glc-R oligosaccharide outer core of lgtB LPS with the dendritic-cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) lectin receptor. Activation of DC-SIGN with this novel oligosaccharide ligand skewed T-cell responses driven by DC towards T helper type 1 activity. Thus, the use of lgtB LPS may provide a powerful instrument to selectively induce the desired arm of the immune response and potentially increase vaccine efficacy.

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KW - Dendritic Cells/immunology

KW - Humans

KW - Lectins, C-Type/metabolism

KW - Lipopolysaccharides/metabolism

KW - Mutation

KW - Neisseria meningitidis/genetics

KW - Oligosaccharides/genetics

KW - Receptors, Cell Surface/metabolism

KW - Th1 Cells/cytology

KW - Th2 Cells/cytology

U2 - 10.1111/j.1462-5822.2005.00623.x

DO - 10.1111/j.1462-5822.2005.00623.x

M3 - Article

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EP - 325

JO - Cellular Microbiology

JF - Cellular Microbiology

SN - 1462-5814

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