Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function

Liana Steeghs, Sandra J van Vliet, Heli Uronen-Hansson, Andries van Mourik, Anneke Engering, Martha Sanchez-Hernandez, Nigel Klein, Robin Callard, Jos P M van Putten, Peter van der Ley, Yvette van Kooyk, Jan G J van de Winkel

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Abstract

Neisseria meningitidis lipopolysaccharide (LPS) has been identified as a major determinant of dendritic cell (DC) function. Here we report that one of a series of meningococcal mutants with defined truncations in the lacto-N-neotetraose outer core of the LPS exhibited unique strong adhesion and internalization properties towards DC. These properties were mediated by interaction of the GlcNAc(beta1-3)-Gal(beta1-4)-Glc-R oligosaccharide outer core of lgtB LPS with the dendritic-cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) lectin receptor. Activation of DC-SIGN with this novel oligosaccharide ligand skewed T-cell responses driven by DC towards T helper type 1 activity. Thus, the use of lgtB LPS may provide a powerful instrument to selectively induce the desired arm of the immune response and potentially increase vaccine efficacy.

Original languageEnglish
Pages (from-to)316-25
Number of pages10
JournalCellular Microbiology
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 2006

Cite this

Steeghs, L., van Vliet, S. J., Uronen-Hansson, H., van Mourik, A., Engering, A., Sanchez-Hernandez, M., ... van de Winkel, J. G. J. (2006). Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function. Cellular Microbiology, 8(2), 316-25. https://doi.org/10.1111/j.1462-5822.2005.00623.x