NEUROD1 acts in vitro as an upstream regulator of NEUROD2 in trophoblast cells

Bart A. Westerman; Aniska Chhatta; Ankie Poutsma; Thijs Van Vegchel; Cees B.M. Oudejans

doi:10.1016/j.bbaexp.2003.10.002

NEUROD1 acts in vitro as an upstream regulator of NEUROD2 in trophoblast cells

Bart A. Westerman, Aniska Chhatta, Ankie Poutsma, Thijs Van Vegchel, Cees B.M. Oudejans^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The basic helix-loop-helix (bHLH) transcription factors NEUROD1, NEUROD2 and ATH2 are expressed during first trimester human placental development. We determined the transactivation potential of each of these factors in trophoblasts by measuring changes in the endogenous gene activity using absolute quantification by real-time quantitative reverse transcriptase- polymerase chain reaction (RT-PCR) after transient transfection. In these assays, NEUROD1 was found to transiently transactivate NEUROD2 in trophoblast cells. Promotor truncation assays, using luciferase constructs, showed the presence of two domains in the NEUROD2 promotor, which showed increased activity after NeuroD1 transfection. Each of these NeuroD1-responsive domains contains an E-box sequence. The NEUROD2 transactivation data fit with the spatial expression pattern of NEUROD1 and NEUROD2, since they are expressed in endovascular trophoblasts. This expression pattern, as well as the present transactivation results, might suggest the presence of a NEUROD differentiation cascade during first trimester human placental development.

Original language	English
Pages (from-to)	96-103
Number of pages	8
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1676
Issue number	1
DOIs	https://doi.org/10.1016/j.bbaexp.2003.10.002
Publication status	Published - 5 Jan 2004

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10.1016/j.bbaexp.2003.10.002

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@article{dab624d2eef54f6f95897e0a895b27df,

title = "NEUROD1 acts in vitro as an upstream regulator of NEUROD2 in trophoblast cells",

abstract = "The basic helix-loop-helix (bHLH) transcription factors NEUROD1, NEUROD2 and ATH2 are expressed during first trimester human placental development. We determined the transactivation potential of each of these factors in trophoblasts by measuring changes in the endogenous gene activity using absolute quantification by real-time quantitative reverse transcriptase- polymerase chain reaction (RT-PCR) after transient transfection. In these assays, NEUROD1 was found to transiently transactivate NEUROD2 in trophoblast cells. Promotor truncation assays, using luciferase constructs, showed the presence of two domains in the NEUROD2 promotor, which showed increased activity after NeuroD1 transfection. Each of these NeuroD1-responsive domains contains an E-box sequence. The NEUROD2 transactivation data fit with the spatial expression pattern of NEUROD1 and NEUROD2, since they are expressed in endovascular trophoblasts. This expression pattern, as well as the present transactivation results, might suggest the presence of a NEUROD differentiation cascade during first trimester human placental development.",

keywords = "ASCL2, ATH2, Basic helix-loop-helix, Endovascular, Extravillous trophoblast, NEUROD1, NEUROD2, Transcription factor",

author = "Westerman, {Bart A.} and Aniska Chhatta and Ankie Poutsma and {Van Vegchel}, Thijs and Oudejans, {Cees B.M.}",

year = "2004",

month = jan,

day = "5",

doi = "10.1016/j.bbaexp.2003.10.002",

language = "English",

volume = "1676",

pages = "96--103",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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T1 - NEUROD1 acts in vitro as an upstream regulator of NEUROD2 in trophoblast cells

AU - Westerman, Bart A.

AU - Chhatta, Aniska

AU - Poutsma, Ankie

AU - Van Vegchel, Thijs

AU - Oudejans, Cees B.M.

PY - 2004/1/5

Y1 - 2004/1/5

N2 - The basic helix-loop-helix (bHLH) transcription factors NEUROD1, NEUROD2 and ATH2 are expressed during first trimester human placental development. We determined the transactivation potential of each of these factors in trophoblasts by measuring changes in the endogenous gene activity using absolute quantification by real-time quantitative reverse transcriptase- polymerase chain reaction (RT-PCR) after transient transfection. In these assays, NEUROD1 was found to transiently transactivate NEUROD2 in trophoblast cells. Promotor truncation assays, using luciferase constructs, showed the presence of two domains in the NEUROD2 promotor, which showed increased activity after NeuroD1 transfection. Each of these NeuroD1-responsive domains contains an E-box sequence. The NEUROD2 transactivation data fit with the spatial expression pattern of NEUROD1 and NEUROD2, since they are expressed in endovascular trophoblasts. This expression pattern, as well as the present transactivation results, might suggest the presence of a NEUROD differentiation cascade during first trimester human placental development.

AB - The basic helix-loop-helix (bHLH) transcription factors NEUROD1, NEUROD2 and ATH2 are expressed during first trimester human placental development. We determined the transactivation potential of each of these factors in trophoblasts by measuring changes in the endogenous gene activity using absolute quantification by real-time quantitative reverse transcriptase- polymerase chain reaction (RT-PCR) after transient transfection. In these assays, NEUROD1 was found to transiently transactivate NEUROD2 in trophoblast cells. Promotor truncation assays, using luciferase constructs, showed the presence of two domains in the NEUROD2 promotor, which showed increased activity after NeuroD1 transfection. Each of these NeuroD1-responsive domains contains an E-box sequence. The NEUROD2 transactivation data fit with the spatial expression pattern of NEUROD1 and NEUROD2, since they are expressed in endovascular trophoblasts. This expression pattern, as well as the present transactivation results, might suggest the presence of a NEUROD differentiation cascade during first trimester human placental development.

KW - ASCL2

KW - ATH2

KW - Basic helix-loop-helix

KW - Endovascular

KW - Extravillous trophoblast

KW - NEUROD1

KW - NEUROD2

KW - Transcription factor

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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