TY - JOUR
T1 - NEUROD1 acts in vitro as an upstream regulator of NEUROD2 in trophoblast cells
AU - Westerman, Bart A.
AU - Chhatta, Aniska
AU - Poutsma, Ankie
AU - Van Vegchel, Thijs
AU - Oudejans, Cees B.M.
PY - 2004/1/5
Y1 - 2004/1/5
N2 - The basic helix-loop-helix (bHLH) transcription factors NEUROD1, NEUROD2 and ATH2 are expressed during first trimester human placental development. We determined the transactivation potential of each of these factors in trophoblasts by measuring changes in the endogenous gene activity using absolute quantification by real-time quantitative reverse transcriptase- polymerase chain reaction (RT-PCR) after transient transfection. In these assays, NEUROD1 was found to transiently transactivate NEUROD2 in trophoblast cells. Promotor truncation assays, using luciferase constructs, showed the presence of two domains in the NEUROD2 promotor, which showed increased activity after NeuroD1 transfection. Each of these NeuroD1-responsive domains contains an E-box sequence. The NEUROD2 transactivation data fit with the spatial expression pattern of NEUROD1 and NEUROD2, since they are expressed in endovascular trophoblasts. This expression pattern, as well as the present transactivation results, might suggest the presence of a NEUROD differentiation cascade during first trimester human placental development.
AB - The basic helix-loop-helix (bHLH) transcription factors NEUROD1, NEUROD2 and ATH2 are expressed during first trimester human placental development. We determined the transactivation potential of each of these factors in trophoblasts by measuring changes in the endogenous gene activity using absolute quantification by real-time quantitative reverse transcriptase- polymerase chain reaction (RT-PCR) after transient transfection. In these assays, NEUROD1 was found to transiently transactivate NEUROD2 in trophoblast cells. Promotor truncation assays, using luciferase constructs, showed the presence of two domains in the NEUROD2 promotor, which showed increased activity after NeuroD1 transfection. Each of these NeuroD1-responsive domains contains an E-box sequence. The NEUROD2 transactivation data fit with the spatial expression pattern of NEUROD1 and NEUROD2, since they are expressed in endovascular trophoblasts. This expression pattern, as well as the present transactivation results, might suggest the presence of a NEUROD differentiation cascade during first trimester human placental development.
KW - ASCL2
KW - ATH2
KW - Basic helix-loop-helix
KW - Endovascular
KW - Extravillous trophoblast
KW - NEUROD1
KW - NEUROD2
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=1642347606&partnerID=8YFLogxK
U2 - 10.1016/j.bbaexp.2003.10.002
DO - 10.1016/j.bbaexp.2003.10.002
M3 - Article
C2 - 14732494
AN - SCOPUS:1642347606
VL - 1676
SP - 96
EP - 103
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -