Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates

Medya Mara Shikhagaie, Åsa K. Björklund, Jenny Mjösberg, Jonas S. Erjefält, Anne S. Cornelissen, Xavier Romero Ros, Suzanne M. Bal, Jasper J. Koning, Reina E. Mebius, Michiko Mori, Melanie Bruchard, Bianca Blom, Hergen Spits

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1 cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.

LanguageEnglish
Pages1761-1773
Number of pages13
JournalCell Reports
Volume18
Issue number7
DOIs
Publication statusPublished - 14 Feb 2017

Cite this

Shikhagaie, M. M., Björklund, Å. K., Mjösberg, J., Erjefält, J. S., Cornelissen, A. S., Ros, X. R., ... Spits, H. (2017). Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates. Cell Reports, 18(7), 1761-1773. https://doi.org/10.1016/j.celrep.2017.01.063
Shikhagaie, Medya Mara ; Björklund, Åsa K. ; Mjösberg, Jenny ; Erjefält, Jonas S. ; Cornelissen, Anne S. ; Ros, Xavier Romero ; Bal, Suzanne M. ; Koning, Jasper J. ; Mebius, Reina E. ; Mori, Michiko ; Bruchard, Melanie ; Blom, Bianca ; Spits, Hergen. / Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates. In: Cell Reports. 2017 ; Vol. 18, No. 7. pp. 1761-1773.
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title = "Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates",
abstract = "Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1− cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.",
keywords = "chemotaxis, HEVs, high endothelial venues, human innate lymphoid cells 3, ILC3, LA, LTi, lymphoid tissue inducer cells, neuropilin-1, NRP1, smoke-induced pulmonary lymphoid aggregates, vascular endothelial growth factor A, VEGF-A",
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Shikhagaie, MM, Björklund, ÅK, Mjösberg, J, Erjefält, JS, Cornelissen, AS, Ros, XR, Bal, SM, Koning, JJ, Mebius, RE, Mori, M, Bruchard, M, Blom, B & Spits, H 2017, 'Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates', Cell Reports, vol. 18, no. 7, pp. 1761-1773. https://doi.org/10.1016/j.celrep.2017.01.063

Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates. / Shikhagaie, Medya Mara; Björklund, Åsa K.; Mjösberg, Jenny; Erjefält, Jonas S.; Cornelissen, Anne S.; Ros, Xavier Romero; Bal, Suzanne M.; Koning, Jasper J.; Mebius, Reina E.; Mori, Michiko; Bruchard, Melanie; Blom, Bianca; Spits, Hergen.

In: Cell Reports, Vol. 18, No. 7, 14.02.2017, p. 1761-1773.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates

AU - Shikhagaie, Medya Mara

AU - Björklund, Åsa K.

AU - Mjösberg, Jenny

AU - Erjefält, Jonas S.

AU - Cornelissen, Anne S.

AU - Ros, Xavier Romero

AU - Bal, Suzanne M.

AU - Koning, Jasper J.

AU - Mebius, Reina E.

AU - Mori, Michiko

AU - Bruchard, Melanie

AU - Blom, Bianca

AU - Spits, Hergen

PY - 2017/2/14

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N2 - Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1− cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.

AB - Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1− cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.

KW - chemotaxis

KW - HEVs

KW - high endothelial venues

KW - human innate lymphoid cells 3

KW - ILC3

KW - LA

KW - LTi

KW - lymphoid tissue inducer cells

KW - neuropilin-1

KW - NRP1

KW - smoke-induced pulmonary lymphoid aggregates

KW - vascular endothelial growth factor A

KW - VEGF-A

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DO - 10.1016/j.celrep.2017.01.063

M3 - Article

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SP - 1761

EP - 1773

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T2 - Cell Reports

JF - Cell Reports

SN - 2211-1247

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