Nickel-responding T cells are CD4+ CLA+ CB45RO+ and express chemokine receptors CXCR3, CCR4 and CCR10

H. Moed, D. M. Boorsma, T. J. Stoof, B. M.E. Von Blomberg, D. P. Bruynzeel, R. J. Scheper, S. Gibbs, T. Rustemeyer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: Whereas T lymphocytes are widely accepted as effector cells determining the pathogenesis of allergic contact dermatitis, contradictory results have been found regarding the roles of different T-cell subsets. The use of various experimental models, involving long-term cultured T-cell lines or clones, may explain these contradictory results. Objective: To investigate the involvement of distinct T-cell subsets in patients with nickel contact allergy. Methods: Different T-cell subsets were directly isolated from peripheral blood mononuclear cells (PBMCs) of nickel-allergic patients, and their proliferative capacity, type-1 or type-2 cytokine secretion [measured by interferon (IFN)-γ or interleukin (IL)-5 release] and phenotypical marker expression were analysed after stimulation with nickel. Results: Only CD4+ CLA+ CD45RO+ and not CD8+ T cells proliferate and produce both type-1 (IFN-γ) and type-2 (IL-5) cytokines in response to nickel. Moreover, cells expressing the marker CLA in combination with CD4, CD45RO or CD69 are increased after nickel-specific stimulation. Interestingly, in addition, CD45RA+ CLA+ cells showed an increased frequency after allergen-specific stimulation. Analysis of nickel-reactive T cells for expression of distinct chemokine receptors showed that both proliferative capacity and cytokine production are restricted to subsets expressing CXCR3, CCR4 but not CCR6. Fluorescence-activated cell sorting analysis of chemokine receptors expressed on nickel-stimulated T cells confirmed these results; a subset of T cells expressing CLA and CXCR3, CCR4 and, most importantly, CCR10 increased in response to allergen, while these CLA+ nickel-reactive T cells were all negative for CCR6. Conclusions: These findings demonstrate that freshly isolated nickel-reactive T cells can be characterized as CD4+ CLA+ memory T cells which express the chemokine receptors CXCR3, CCR4 and CCR10, but not CCR6.

Original languageEnglish
Pages (from-to)32-41
Number of pages10
JournalBritish Journal of Dermatology
Volume151
Issue number1
DOIs
Publication statusPublished - 1 Jul 2004

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