Nitric oxide modulates neuromuscular transmission during hypoxia in rat diaphragm

Xiaoping Zhu, Leo M.A. Heunks, Leo Ennen, Herwin A. Machiels, Henricus F.M. Van Der Heijden, P. N.Richard Dekhuijzen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Hypoxia impairs neuromuscular transmission in the rat diaphragm. In previous studies, we have shown that nitric oxide (NO) plays a role in force modulation of the diaphragm under hypoxic conditions. The role of NO, a neurotransmitter, on neurotransmission in skeletal muscle under hypoxic conditions is unknown. The effects of the NO synthase (NOS) inhibitor nomega-nitro-L-arginine (L-NNA, 1 mM) and the NO donor spermine NONOate (Sp-NO, 1 mM) were evaluated on neurotransmission failure during nonfatiguing and fatiguing contractions of the rat diaphragm under hypoxic (PO2 ∼ 5.8 kPa) and hyperoxic conditions (PO2 ∼ 64.0 kPa). Hypoxia impaired force generated by both muscle stimulation at 40 Hz (P40M) and by nerve stimulation at 40 Hz (P40N). The effect of hypoxia in the latter was more pronounced. L-NNA increased P40N whereas Sp-NO decreased P40N during hypoxia. In contrast, neither L-NNA nor Sp-NO affected P40N during hyperoxia. L-NNA only slightly reduced neurotransmission failure during fatiguing contractions under hyperoxic conditions. Consequently, neurotransmission failure assessed by comparing force loss during repetitive nerve simulation and superimposed direct muscle stimulation was more pronounced in hypoxia, which was alleviated by L-NNA and aggravated by Sp-NO. These data provide insight in the underlying mechanisms of hypoxia-induced neurotransmission failure. This is important as respiratory muscle failure may result from hypoxia in vivo.

Original languageEnglish
Pages (from-to)104-112
Number of pages9
JournalMuscle and Nerve
Volume33
Issue number1
DOIs
Publication statusPublished - 1 Jan 2006

Cite this

Zhu, X., Heunks, L. M. A., Ennen, L., Machiels, H. A., Van Der Heijden, H. F. M., & Dekhuijzen, P. N. R. (2006). Nitric oxide modulates neuromuscular transmission during hypoxia in rat diaphragm. Muscle and Nerve, 33(1), 104-112. https://doi.org/10.1002/mus.20445
Zhu, Xiaoping ; Heunks, Leo M.A. ; Ennen, Leo ; Machiels, Herwin A. ; Van Der Heijden, Henricus F.M. ; Dekhuijzen, P. N.Richard. / Nitric oxide modulates neuromuscular transmission during hypoxia in rat diaphragm. In: Muscle and Nerve. 2006 ; Vol. 33, No. 1. pp. 104-112.
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Zhu, X, Heunks, LMA, Ennen, L, Machiels, HA, Van Der Heijden, HFM & Dekhuijzen, PNR 2006, 'Nitric oxide modulates neuromuscular transmission during hypoxia in rat diaphragm' Muscle and Nerve, vol. 33, no. 1, pp. 104-112. https://doi.org/10.1002/mus.20445

Nitric oxide modulates neuromuscular transmission during hypoxia in rat diaphragm. / Zhu, Xiaoping; Heunks, Leo M.A.; Ennen, Leo; Machiels, Herwin A.; Van Der Heijden, Henricus F.M.; Dekhuijzen, P. N.Richard.

In: Muscle and Nerve, Vol. 33, No. 1, 01.01.2006, p. 104-112.

Research output: Contribution to journalArticleAcademicpeer-review

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Zhu X, Heunks LMA, Ennen L, Machiels HA, Van Der Heijden HFM, Dekhuijzen PNR. Nitric oxide modulates neuromuscular transmission during hypoxia in rat diaphragm. Muscle and Nerve. 2006 Jan 1;33(1):104-112. https://doi.org/10.1002/mus.20445