No evidence for involvement of plasma proteins or blood-borne cells in amyloid plaque formation in scrapie-affected mice - An immunohistoperoxidase study

P. Eikelenboom*, J. R. Scott, P. A. McBride, J. M. Rozemuller, M. E. Bruce, H. Fraser

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The present study was designed to investigate blood-brain permeability and the possible involvement of plasma proteins and blood-borne cells in amyloid plaque formation in scrapie-affected mice. No abnormal extravasation of intravenously injected horseradish peroxidase (HRP) was found and with immunocytochemical techniques no plasma proteins were detected in neuropil from scrapie-affected mice. In contrast to an earlier report, these findings suggest that the blood-brain barrier is essentially intact in scrapie-affected mice. Using immunohistochemical and enzyme histochemical methods no cells belonging to the monocyte-macrophage lineage were detected in association with amyloid plaques. Thus, by these methods there was no evidence that plasma proteins or blood-borne cells are involved in amyloid plaque formation in scrapie-affected mice. However, astrocytes were consistently found to be associated with amyloid plaques at all stages of their development.

Original languageEnglish
Pages (from-to)251-256
Number of pages6
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume53
Issue number1
DOIs
Publication statusPublished - 1 Dec 1987

Cite this