N(omega)-(carboxymethyl)lysine depositions in human aortic heart valves: similarities with atherosclerotic blood vessels

Alexi Baidoshvili, Hans W M Niessen, Wim Stooker, Rien A J M Huybregts, C Erik Hack, Jan A Rauwerda, Chris J L M Meijer, Leon Eijsman, Victor W M van Hinsbergh, Casper G Schalkwijk

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Recent studies indicate a role of atherosclerosis-like changes involved in the pathogenesis of aortic valve stenosis. Interestingly, one of the major advanced glycation end products (AGEs), N(omega)-(carboxymethyl)lysine (CML) has been related to the process of atherosclerosis in blood vessels. In the present study, we have analyzed the presence of CML in degenerative altered aortic valves with atherosclerosis-like changes, and in degenerated mitral valves without atherosclerosis-like changes, derived from patients suffering from acute rheumatism during childhood. Degenerated and non-degenerated valves were derived from autopsy or obtained during cardiac surgery. The presence of CML was examined by immunohistochemistry. CML was found on the endothelium and fibroblasts in control aortic and mitral valves. Minor differences in CML staining were observed between control and degeneratively affected mitral valves. In contrast, in degenerated aortic valves, CML accumulation was found in macrophages and on calcification sites, comparable to that in atherosclerotic arteries, while the presence of CML staining on the endothelium and fibroblasts was significantly less as compared with control aortic valves. Our data support the hypothesis that the process of degeneration of aortic valves resembles that of atherosclerosis in blood vessels. They suggest that CML also plays a role in the process of atherosclerosis in aortic valves.

Original languageEnglish
Pages (from-to)287-92
Number of pages6
JournalAtherosclerosis
Volume174
Issue number2
DOIs
Publication statusPublished - Jun 2004

Cite this

Baidoshvili, Alexi ; Niessen, Hans W M ; Stooker, Wim ; Huybregts, Rien A J M ; Hack, C Erik ; Rauwerda, Jan A ; Meijer, Chris J L M ; Eijsman, Leon ; van Hinsbergh, Victor W M ; Schalkwijk, Casper G. / N(omega)-(carboxymethyl)lysine depositions in human aortic heart valves : similarities with atherosclerotic blood vessels. In: Atherosclerosis. 2004 ; Vol. 174, No. 2. pp. 287-92.
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abstract = "Recent studies indicate a role of atherosclerosis-like changes involved in the pathogenesis of aortic valve stenosis. Interestingly, one of the major advanced glycation end products (AGEs), N(omega)-(carboxymethyl)lysine (CML) has been related to the process of atherosclerosis in blood vessels. In the present study, we have analyzed the presence of CML in degenerative altered aortic valves with atherosclerosis-like changes, and in degenerated mitral valves without atherosclerosis-like changes, derived from patients suffering from acute rheumatism during childhood. Degenerated and non-degenerated valves were derived from autopsy or obtained during cardiac surgery. The presence of CML was examined by immunohistochemistry. CML was found on the endothelium and fibroblasts in control aortic and mitral valves. Minor differences in CML staining were observed between control and degeneratively affected mitral valves. In contrast, in degenerated aortic valves, CML accumulation was found in macrophages and on calcification sites, comparable to that in atherosclerotic arteries, while the presence of CML staining on the endothelium and fibroblasts was significantly less as compared with control aortic valves. Our data support the hypothesis that the process of degeneration of aortic valves resembles that of atherosclerosis in blood vessels. They suggest that CML also plays a role in the process of atherosclerosis in aortic valves.",
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N(omega)-(carboxymethyl)lysine depositions in human aortic heart valves : similarities with atherosclerotic blood vessels. / Baidoshvili, Alexi; Niessen, Hans W M; Stooker, Wim; Huybregts, Rien A J M; Hack, C Erik; Rauwerda, Jan A; Meijer, Chris J L M; Eijsman, Leon; van Hinsbergh, Victor W M; Schalkwijk, Casper G.

In: Atherosclerosis, Vol. 174, No. 2, 06.2004, p. 287-92.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - N(omega)-(carboxymethyl)lysine depositions in human aortic heart valves

T2 - similarities with atherosclerotic blood vessels

AU - Baidoshvili, Alexi

AU - Niessen, Hans W M

AU - Stooker, Wim

AU - Huybregts, Rien A J M

AU - Hack, C Erik

AU - Rauwerda, Jan A

AU - Meijer, Chris J L M

AU - Eijsman, Leon

AU - van Hinsbergh, Victor W M

AU - Schalkwijk, Casper G

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N2 - Recent studies indicate a role of atherosclerosis-like changes involved in the pathogenesis of aortic valve stenosis. Interestingly, one of the major advanced glycation end products (AGEs), N(omega)-(carboxymethyl)lysine (CML) has been related to the process of atherosclerosis in blood vessels. In the present study, we have analyzed the presence of CML in degenerative altered aortic valves with atherosclerosis-like changes, and in degenerated mitral valves without atherosclerosis-like changes, derived from patients suffering from acute rheumatism during childhood. Degenerated and non-degenerated valves were derived from autopsy or obtained during cardiac surgery. The presence of CML was examined by immunohistochemistry. CML was found on the endothelium and fibroblasts in control aortic and mitral valves. Minor differences in CML staining were observed between control and degeneratively affected mitral valves. In contrast, in degenerated aortic valves, CML accumulation was found in macrophages and on calcification sites, comparable to that in atherosclerotic arteries, while the presence of CML staining on the endothelium and fibroblasts was significantly less as compared with control aortic valves. Our data support the hypothesis that the process of degeneration of aortic valves resembles that of atherosclerosis in blood vessels. They suggest that CML also plays a role in the process of atherosclerosis in aortic valves.

AB - Recent studies indicate a role of atherosclerosis-like changes involved in the pathogenesis of aortic valve stenosis. Interestingly, one of the major advanced glycation end products (AGEs), N(omega)-(carboxymethyl)lysine (CML) has been related to the process of atherosclerosis in blood vessels. In the present study, we have analyzed the presence of CML in degenerative altered aortic valves with atherosclerosis-like changes, and in degenerated mitral valves without atherosclerosis-like changes, derived from patients suffering from acute rheumatism during childhood. Degenerated and non-degenerated valves were derived from autopsy or obtained during cardiac surgery. The presence of CML was examined by immunohistochemistry. CML was found on the endothelium and fibroblasts in control aortic and mitral valves. Minor differences in CML staining were observed between control and degeneratively affected mitral valves. In contrast, in degenerated aortic valves, CML accumulation was found in macrophages and on calcification sites, comparable to that in atherosclerotic arteries, while the presence of CML staining on the endothelium and fibroblasts was significantly less as compared with control aortic valves. Our data support the hypothesis that the process of degeneration of aortic valves resembles that of atherosclerosis in blood vessels. They suggest that CML also plays a role in the process of atherosclerosis in aortic valves.

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KW - Biomarkers

KW - Blood Vessels

KW - Cadaver

KW - Culture Techniques

KW - Endothelium, Vascular

KW - Female

KW - Fibroblasts

KW - Glycation End Products, Advanced

KW - Humans

KW - Immunohistochemistry

KW - Lysine

KW - Male

KW - Middle Aged

KW - Mitral Valve

KW - Probability

KW - Reference Values

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.atherosclerosis.2004.02.012

DO - 10.1016/j.atherosclerosis.2004.02.012

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VL - 174

SP - 287

EP - 292

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

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