Non-lymphoid and lymphoid cells in acute, chronic and relapsing experimental colitis

M. J.H.J. Palmen*, L. A. Dieleman, M. B. Van Der Ende, A. Uyterlinde, A. S. Pena, S. G.M. Meuwissen, E. P. Van Rees

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


In rodents, intracolonic administration of ethanol 30% induces an acute colitis, while administration of 2,4,6-trinitrobenzene sulphonic acid (TNBS) in ethanol induces a longer lasting colitis. In the acute and chronic stages of experimental colitis, lymphoid and non-lymphoid cells were studied in the colon by immunohistochemistry. During the acute inflammation a high damage score of the colon was observed, which was related to an increase in the number of macrophages and granulocytes. Also a change in distributional patterns of macrophage subpopulations was found. The chronic stage of TNBS-ethanol-induced colitis was characterized by an increase in the number of lymphocytes, especially T cells. These data suggest that macrophages and granulocytes are important in the acute phase of experimental colitis, while lymphocytes play a pivotal role in the chronic stage. As most inflammatory bowel disease (IBD) patients have relapses during the chronic disease, we attempted to induce a relapse during experimental colitis by giving a second i.p. or s.c. dose of TNBS. This resulted in increased damage scores of the colon, new areas of ulceration and a further increase in macrophage numbers. No effect on the number of granulocytes was seen. These results indicate that it is possible to mimic relapses in experimental colitis by a second administration of TNBS, and suggest that the rats had been sensitized by the first dose of TNBS, given into the colon.

Original languageEnglish
Pages (from-to)226-232
Number of pages7
JournalClinical and Experimental Immunology
Issue number2
Publication statusPublished - 1995

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