TY - JOUR
T1 - Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET
T2 - Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [11C]MPDX
AU - Sijbesma, Jürgen W.A.
AU - Zhou, Xiaoyun
AU - Vállez García, David
AU - Houwertjes, Martin C.
AU - Doorduin, Janine
AU - Kwizera, Chantal
AU - Maas, Bram
AU - Meerlo, Peter
AU - Dierckx, Rudi A.
AU - Slart, Riemer H.J.A.
AU - Elsinga, Philip H.
AU - van Waarde, Aren
N1 - Publisher Copyright:
© 2016, The Author(s).
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Purpose: Small animal positron emission tomography (PET) can be used to detect small changes in neuroreceptor availability. This often requires rapid arterial blood sampling. However, current catheterization procedures do not allow repeated blood sampling. We have developed a procedure which allows arterial sampling on repeated occasions in the same animal. Procedures: Eleven male Wistar rats were two times catheterized via a superficial branch of a femoral artery and scanned with [11C]MPDX and blood sampling. PET images were co-registered to a magnetic resonance imaging (MRI) template. Regional tracer distribution volumes (VT) in the brain were calculated by the Logan analysis. The procedure was repeated after 1 week. Results: Surgery was successful in 90 % of the cases, and discomfort was minor. The VT data showed small differences between test and retest, low between subject variability, and a strong agreement between and within subjects. Conclusion: Repeated quantitative imaging with a high reproducibility is possible with this approach.
AB - Purpose: Small animal positron emission tomography (PET) can be used to detect small changes in neuroreceptor availability. This often requires rapid arterial blood sampling. However, current catheterization procedures do not allow repeated blood sampling. We have developed a procedure which allows arterial sampling on repeated occasions in the same animal. Procedures: Eleven male Wistar rats were two times catheterized via a superficial branch of a femoral artery and scanned with [11C]MPDX and blood sampling. PET images were co-registered to a magnetic resonance imaging (MRI) template. Regional tracer distribution volumes (VT) in the brain were calculated by the Logan analysis. The procedure was repeated after 1 week. Results: Surgery was successful in 90 % of the cases, and discomfort was minor. The VT data showed small differences between test and retest, low between subject variability, and a strong agreement between and within subjects. Conclusion: Repeated quantitative imaging with a high reproducibility is possible with this approach.
KW - Adenosine A1 receptor
KW - Arterial blood sampling
KW - Brain
KW - Positron emission tomography
KW - Test-retest reproducibility
UR - http://www.scopus.com/inward/record.url?scp=84964317993&partnerID=8YFLogxK
U2 - 10.1007/s11307-016-0954-9
DO - 10.1007/s11307-016-0954-9
M3 - Article
C2 - 27091332
AN - SCOPUS:84964317993
SN - 1536-1632
VL - 18
SP - 715
EP - 723
JO - Molecular Imaging and Biology
JF - Molecular Imaging and Biology
IS - 5
ER -