Oligonucleotide array comparative genomic hybridization

Paul Van Den Ijssel, Bauke Ylstra

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

The array CGH technique (array comparative genome hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. Here, we present validated protocols using in-house spotted oligonucleotide libraries for array CGH. This oligo array CGH platform yields reproducible results and is capable of detecting single copy gains, multicopy amplifications as well as homozygous and heterozygous deletions with high resolution. The protocol allows as little as 300 ng of input DNA, which makes the procedure valuable for small clinical samples and is also functional for DNA samples obtained from archival tissues.

Original languageEnglish
Title of host publicationComparative Genomics
EditorsBergman H. Nicholas
Pages207-221
Number of pages15
DOIs
Publication statusPublished - 1 Nov 2007

Publication series

NameMethods in Molecular Biology
Volume396
ISSN (Print)1064-3745

Cite this

Van Den Ijssel, P., & Ylstra, B. (2007). Oligonucleotide array comparative genomic hybridization. In B. H. Nicholas (Ed.), Comparative Genomics (pp. 207-221). (Methods in Molecular Biology; Vol. 396). https://doi.org/10.1385/1-59745-515-6:207