Ontogeny of μ-, δ- and κ-opioid receptors mediating inhibition of neurotransmitter release and adenylate cyclase activity in rat brain

Taco J. De Vries, François Hogenboom, Arie H. Mulder, Anton N M Schoffelmeer

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The ontogeny was examined of functional opioid receptors mediating presynaptic inhibition of neurotransmitter release and inhibition of dopamine (DA)-sensitive adenylate cyclase in the rat brain, using highly selective agonists for μ-, δ- and κ-receptors. On gestational day 17 (E17) strong inhibitory effects of the selective μ-agonist DAGO on the electrically evoked release of [3H]noradrenaline from cortical slices and of the selective κ-agonist U-50,488 on the electrically evoked release of [3H]DA from striatal slices were found. Electrically evoked release of [3H]acetylcholine from striatal slices was not detectable before postnatal day 7 (P7), but on that day it was already strongly inhibited by the selective δ-agonist DPDPE. Although μ- and δ-opioid receptors coupled to DA-sensitive adenylate cyclase in the striatum are likely to be physically associated in an opioid receptor complex in the adult, they were found to develop asynchronously. Whereas selective activation of μ-receptors with DAGO resulted in an inhibition of D1 dopamine receptor-stimulated adenylate cyclase activity on E17, activation of δ-receptors with DPDPE was not effective until P14. This study confirms the early appearance of μ- and κ-opioid receptors and the relatively late development of δ-opioid receptors in the rat brain. Most importantly, it shows that in an early stage of development opioids are already able to mediate modulation of noradrenergic (via activation of μ-receptors) and dopaminergic (via activation of μ- and κ-receptors) neurotransmission processes. Therefore, these opioid receptor types could play a role in brain development and/or developmental disturbances.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalDEVELOPMENTAL BRAIN RESEARCH
Volume54
Issue number1
DOIs
Publication statusPublished - 1 Jun 1990

Cite this

@article{6dacc80b0e5a4ebdb1b8f9bb00f2017f,
title = "Ontogeny of μ-, δ- and κ-opioid receptors mediating inhibition of neurotransmitter release and adenylate cyclase activity in rat brain",
abstract = "The ontogeny was examined of functional opioid receptors mediating presynaptic inhibition of neurotransmitter release and inhibition of dopamine (DA)-sensitive adenylate cyclase in the rat brain, using highly selective agonists for μ-, δ- and κ-receptors. On gestational day 17 (E17) strong inhibitory effects of the selective μ-agonist DAGO on the electrically evoked release of [3H]noradrenaline from cortical slices and of the selective κ-agonist U-50,488 on the electrically evoked release of [3H]DA from striatal slices were found. Electrically evoked release of [3H]acetylcholine from striatal slices was not detectable before postnatal day 7 (P7), but on that day it was already strongly inhibited by the selective δ-agonist DPDPE. Although μ- and δ-opioid receptors coupled to DA-sensitive adenylate cyclase in the striatum are likely to be physically associated in an opioid receptor complex in the adult, they were found to develop asynchronously. Whereas selective activation of μ-receptors with DAGO resulted in an inhibition of D1 dopamine receptor-stimulated adenylate cyclase activity on E17, activation of δ-receptors with DPDPE was not effective until P14. This study confirms the early appearance of μ- and κ-opioid receptors and the relatively late development of δ-opioid receptors in the rat brain. Most importantly, it shows that in an early stage of development opioids are already able to mediate modulation of noradrenergic (via activation of μ-receptors) and dopaminergic (via activation of μ- and κ-receptors) neurotransmission processes. Therefore, these opioid receptor types could play a role in brain development and/or developmental disturbances.",
keywords = "Adenylate cyclase, Neurotransmitter release, Ontogeny, Opioid receptor, Rat brain slice",
author = "{De Vries}, {Taco J.} and Fran{\cc}ois Hogenboom and Mulder, {Arie H.} and Schoffelmeer, {Anton N M}",
year = "1990",
month = "6",
day = "1",
doi = "10.1016/0165-3806(90)90065-7",
language = "English",
volume = "54",
pages = "63--69",
journal = "DEVELOPMENTAL BRAIN RESEARCH",
issn = "0165-3806",
publisher = "Elsevier BV",
number = "1",

}

Ontogeny of μ-, δ- and κ-opioid receptors mediating inhibition of neurotransmitter release and adenylate cyclase activity in rat brain. / De Vries, Taco J.; Hogenboom, François; Mulder, Arie H.; Schoffelmeer, Anton N M.

In: DEVELOPMENTAL BRAIN RESEARCH, Vol. 54, No. 1, 01.06.1990, p. 63-69.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Ontogeny of μ-, δ- and κ-opioid receptors mediating inhibition of neurotransmitter release and adenylate cyclase activity in rat brain

AU - De Vries, Taco J.

AU - Hogenboom, François

AU - Mulder, Arie H.

AU - Schoffelmeer, Anton N M

PY - 1990/6/1

Y1 - 1990/6/1

N2 - The ontogeny was examined of functional opioid receptors mediating presynaptic inhibition of neurotransmitter release and inhibition of dopamine (DA)-sensitive adenylate cyclase in the rat brain, using highly selective agonists for μ-, δ- and κ-receptors. On gestational day 17 (E17) strong inhibitory effects of the selective μ-agonist DAGO on the electrically evoked release of [3H]noradrenaline from cortical slices and of the selective κ-agonist U-50,488 on the electrically evoked release of [3H]DA from striatal slices were found. Electrically evoked release of [3H]acetylcholine from striatal slices was not detectable before postnatal day 7 (P7), but on that day it was already strongly inhibited by the selective δ-agonist DPDPE. Although μ- and δ-opioid receptors coupled to DA-sensitive adenylate cyclase in the striatum are likely to be physically associated in an opioid receptor complex in the adult, they were found to develop asynchronously. Whereas selective activation of μ-receptors with DAGO resulted in an inhibition of D1 dopamine receptor-stimulated adenylate cyclase activity on E17, activation of δ-receptors with DPDPE was not effective until P14. This study confirms the early appearance of μ- and κ-opioid receptors and the relatively late development of δ-opioid receptors in the rat brain. Most importantly, it shows that in an early stage of development opioids are already able to mediate modulation of noradrenergic (via activation of μ-receptors) and dopaminergic (via activation of μ- and κ-receptors) neurotransmission processes. Therefore, these opioid receptor types could play a role in brain development and/or developmental disturbances.

AB - The ontogeny was examined of functional opioid receptors mediating presynaptic inhibition of neurotransmitter release and inhibition of dopamine (DA)-sensitive adenylate cyclase in the rat brain, using highly selective agonists for μ-, δ- and κ-receptors. On gestational day 17 (E17) strong inhibitory effects of the selective μ-agonist DAGO on the electrically evoked release of [3H]noradrenaline from cortical slices and of the selective κ-agonist U-50,488 on the electrically evoked release of [3H]DA from striatal slices were found. Electrically evoked release of [3H]acetylcholine from striatal slices was not detectable before postnatal day 7 (P7), but on that day it was already strongly inhibited by the selective δ-agonist DPDPE. Although μ- and δ-opioid receptors coupled to DA-sensitive adenylate cyclase in the striatum are likely to be physically associated in an opioid receptor complex in the adult, they were found to develop asynchronously. Whereas selective activation of μ-receptors with DAGO resulted in an inhibition of D1 dopamine receptor-stimulated adenylate cyclase activity on E17, activation of δ-receptors with DPDPE was not effective until P14. This study confirms the early appearance of μ- and κ-opioid receptors and the relatively late development of δ-opioid receptors in the rat brain. Most importantly, it shows that in an early stage of development opioids are already able to mediate modulation of noradrenergic (via activation of μ-receptors) and dopaminergic (via activation of μ- and κ-receptors) neurotransmission processes. Therefore, these opioid receptor types could play a role in brain development and/or developmental disturbances.

KW - Adenylate cyclase

KW - Neurotransmitter release

KW - Ontogeny

KW - Opioid receptor

KW - Rat brain slice

UR - http://www.scopus.com/inward/record.url?scp=0025277428&partnerID=8YFLogxK

U2 - 10.1016/0165-3806(90)90065-7

DO - 10.1016/0165-3806(90)90065-7

M3 - Article

VL - 54

SP - 63

EP - 69

JO - DEVELOPMENTAL BRAIN RESEARCH

JF - DEVELOPMENTAL BRAIN RESEARCH

SN - 0165-3806

IS - 1

ER -