Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats

Donna J. Calu, Alex B. Kawa, Nathan J. Marchant, Brittany M. Navarre, Mark J. Henderson, Billy Chen, Hau Jie Yau, Jennifer M. Bossert, Geoffrey Schoenbaum, Karl Deisseroth, Brandon K. Harvey, Bruce T. Hope, Yavin Shaham

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Relapse to maladaptive eating habits during dieting is often provoked by stress. Recently, we identified a role of dorsal medial prefrontal cortex (mPFC) neurons in stress-induced reinstatement of palatable food seeking in male rats. It is unknown whether endogenous neural activity in dorsal mPFC drives stress-induced reinstatement in female rats. Here, we used an optogenetic approach, in which female rats received bilateral dorsal mPFC microinjections of viral constructs coding light-sensitive eNpHR3.0-eYFP or control eYFP protein and intracranial fiber optic implants. Rats were food restricted and trained to lever press for palatable food pellets. Subsequently, pellets were removed, and lever pressing was extinguished; then the effect of bilateral dorsalmPFClight delivery on reinstatement of food seeking was assessed after injections of the pharmacological stressor yohimbine (anα-2 andrenoceptor antagonist) or pellet priming, a manipulation known to provoke food seeking in hungry rats. Dorsal mPFC light delivery attenuated yohimbine-induced reinstatement of food seeking in eNpHR3.0-injected but not eYFP-injected rats. This optical manipulation had no effect on pellet-priming-induced reinstatement or ongoing food-reinforced responding. Dorsal mPFC light delivery attenuated yohimbine-induced Fos immunoreactivity and disrupted neural activity during in vivo electrophysiological recording in awake rats. Optical stimulation caused significant outward currents and blocked electrically evoked action potentials in eNpHR3.0-injected but not eYFP-injected mPFC hemispheres. Light delivery alone caused no significant inflammatory response in mPFC. These findings indicate that intracranial light delivery in eNpHR3.0 rats disrupts endogenous dorsal mPFC neural activity that plays a role in stress-induced relapse to food seeking in female rats.

Original languageEnglish
Pages (from-to)214-226
Number of pages13
JournalJournal of Neuroscience
Volume33
Issue number1
DOIs
Publication statusPublished - 2 Jan 2013

Cite this

Calu, Donna J. ; Kawa, Alex B. ; Marchant, Nathan J. ; Navarre, Brittany M. ; Henderson, Mark J. ; Chen, Billy ; Yau, Hau Jie ; Bossert, Jennifer M. ; Schoenbaum, Geoffrey ; Deisseroth, Karl ; Harvey, Brandon K. ; Hope, Bruce T. ; Shaham, Yavin. / Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 1. pp. 214-226.
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title = "Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats",
abstract = "Relapse to maladaptive eating habits during dieting is often provoked by stress. Recently, we identified a role of dorsal medial prefrontal cortex (mPFC) neurons in stress-induced reinstatement of palatable food seeking in male rats. It is unknown whether endogenous neural activity in dorsal mPFC drives stress-induced reinstatement in female rats. Here, we used an optogenetic approach, in which female rats received bilateral dorsal mPFC microinjections of viral constructs coding light-sensitive eNpHR3.0-eYFP or control eYFP protein and intracranial fiber optic implants. Rats were food restricted and trained to lever press for palatable food pellets. Subsequently, pellets were removed, and lever pressing was extinguished; then the effect of bilateral dorsalmPFClight delivery on reinstatement of food seeking was assessed after injections of the pharmacological stressor yohimbine (anα-2 andrenoceptor antagonist) or pellet priming, a manipulation known to provoke food seeking in hungry rats. Dorsal mPFC light delivery attenuated yohimbine-induced reinstatement of food seeking in eNpHR3.0-injected but not eYFP-injected rats. This optical manipulation had no effect on pellet-priming-induced reinstatement or ongoing food-reinforced responding. Dorsal mPFC light delivery attenuated yohimbine-induced Fos immunoreactivity and disrupted neural activity during in vivo electrophysiological recording in awake rats. Optical stimulation caused significant outward currents and blocked electrically evoked action potentials in eNpHR3.0-injected but not eYFP-injected mPFC hemispheres. Light delivery alone caused no significant inflammatory response in mPFC. These findings indicate that intracranial light delivery in eNpHR3.0 rats disrupts endogenous dorsal mPFC neural activity that plays a role in stress-induced relapse to food seeking in female rats.",
author = "Calu, {Donna J.} and Kawa, {Alex B.} and Marchant, {Nathan J.} and Navarre, {Brittany M.} and Henderson, {Mark J.} and Billy Chen and Yau, {Hau Jie} and Bossert, {Jennifer M.} and Geoffrey Schoenbaum and Karl Deisseroth and Harvey, {Brandon K.} and Hope, {Bruce T.} and Yavin Shaham",
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Calu, DJ, Kawa, AB, Marchant, NJ, Navarre, BM, Henderson, MJ, Chen, B, Yau, HJ, Bossert, JM, Schoenbaum, G, Deisseroth, K, Harvey, BK, Hope, BT & Shaham, Y 2013, 'Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats' Journal of Neuroscience, vol. 33, no. 1, pp. 214-226. https://doi.org/10.1523/JNEUROSCI.2016-12.2013

Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats. / Calu, Donna J.; Kawa, Alex B.; Marchant, Nathan J.; Navarre, Brittany M.; Henderson, Mark J.; Chen, Billy; Yau, Hau Jie; Bossert, Jennifer M.; Schoenbaum, Geoffrey; Deisseroth, Karl; Harvey, Brandon K.; Hope, Bruce T.; Shaham, Yavin.

In: Journal of Neuroscience, Vol. 33, No. 1, 02.01.2013, p. 214-226.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Calu, Donna J.

AU - Kawa, Alex B.

AU - Marchant, Nathan J.

AU - Navarre, Brittany M.

AU - Henderson, Mark J.

AU - Chen, Billy

AU - Yau, Hau Jie

AU - Bossert, Jennifer M.

AU - Schoenbaum, Geoffrey

AU - Deisseroth, Karl

AU - Harvey, Brandon K.

AU - Hope, Bruce T.

AU - Shaham, Yavin

PY - 2013/1/2

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EP - 226

JO - Journal of Neuroscience

JF - Journal of Neuroscience

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