Pairing bacteroides vulgatus LPS structure with its immunomodulatory effects on human cellular models

Flaviana Di Lorenzo*, Molly D. Pither, Michela Martufi, Ilaria Scarinci, Joan Guzmán-Caldentey, Ewelina Łakomiec, Wojciech Jachymek, Sven C.M. Bruijns, Sonsoles Martín Santamaría, Julia Stephanie Frick, Yvette Van Kooyk, Fabrizio Chiodo, Alba Silipo, Maria Lina Bernardini, Antonio Molinaro

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


The gut microbiota guide the development of the host immune system by setting a systemic threshold for immune activation. Lipopolysaccharides (LPSs) from gut bacteria are able to trigger systemic and local proinflammatory and immunomodulatory responses, and this capability strongly relies on their fine structures. Up to now, only a few LPS structures from gut commensals have been elucidated; therefore, the molecular motifs that may be important for LPS-mammalian cell interactions at the gut level are still obscure. Here, we report on the full structure of the LPS isolated from one of the prominent species of the genus Bacteroides, Bacteroides vulgatus. The LPS turned out to consist of a particular chemical structure based on hypoacylated and mono-phosphorylated lipid A and with a galactofuranose-containing core oligosaccharide and an O-antigen built up of mannose and rhamnose. The evaluation of the immunological properties of this LPS on human in vitro models revealed a very interesting capability to produce anti-inflammatory cytokines and to induce a synergistic action of MD-2/TLR4- and TLR2-mediated signaling pathways.

Original languageEnglish
Pages (from-to)1602-1616
Number of pages15
JournalACS Central Science
Issue number9
Publication statusPublished - 23 Sep 2020

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