Presence of glutathione immunoreactivity in cultured neurones and astrocytes

C H Langeveld, E Schepens, C A Jongenelen, J C Stoof, O P Hjelle, O P Ottersen, B Drukarch

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Although glutathione (GSH) is considered an important antioxidant in the brain, its cellular localization is unclear. In general, neurones are supposed to contain considerably less GSH than astrocytes. We determined biochemically and immunocytochemically the presence of GSH in cultured neurones and astrocytes from the cortex, mesencephalon and striatum. Cortical neurones contained less GSH than astrocytes whereas GSH levels in neurones from the striatum and mesencephalon were comparable to those in astrocytes. Immunocytochemistry showed significant GSH staining in neurones. Fluorescent double staining of GSH and tyrosine hydroxylase revealed that dopaminergic neurones also contained GSH, although apparently at a lower level than other mesencephalic neurones.

Original languageEnglish
Pages (from-to)1833-6
Number of pages4
JournalNeuroreport
Volume7
Issue number11
Publication statusPublished - 29 Jul 1996

Cite this

Langeveld, C. H., Schepens, E., Jongenelen, C. A., Stoof, J. C., Hjelle, O. P., Ottersen, O. P., & Drukarch, B. (1996). Presence of glutathione immunoreactivity in cultured neurones and astrocytes. Neuroreport, 7(11), 1833-6.
Langeveld, C H ; Schepens, E ; Jongenelen, C A ; Stoof, J C ; Hjelle, O P ; Ottersen, O P ; Drukarch, B. / Presence of glutathione immunoreactivity in cultured neurones and astrocytes. In: Neuroreport. 1996 ; Vol. 7, No. 11. pp. 1833-6.
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Langeveld, CH, Schepens, E, Jongenelen, CA, Stoof, JC, Hjelle, OP, Ottersen, OP & Drukarch, B 1996, 'Presence of glutathione immunoreactivity in cultured neurones and astrocytes' Neuroreport, vol. 7, no. 11, pp. 1833-6.

Presence of glutathione immunoreactivity in cultured neurones and astrocytes. / Langeveld, C H; Schepens, E; Jongenelen, C A; Stoof, J C; Hjelle, O P; Ottersen, O P; Drukarch, B.

In: Neuroreport, Vol. 7, No. 11, 29.07.1996, p. 1833-6.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Presence of glutathione immunoreactivity in cultured neurones and astrocytes

AU - Langeveld, C H

AU - Schepens, E

AU - Jongenelen, C A

AU - Stoof, J C

AU - Hjelle, O P

AU - Ottersen, O P

AU - Drukarch, B

PY - 1996/7/29

Y1 - 1996/7/29

N2 - Although glutathione (GSH) is considered an important antioxidant in the brain, its cellular localization is unclear. In general, neurones are supposed to contain considerably less GSH than astrocytes. We determined biochemically and immunocytochemically the presence of GSH in cultured neurones and astrocytes from the cortex, mesencephalon and striatum. Cortical neurones contained less GSH than astrocytes whereas GSH levels in neurones from the striatum and mesencephalon were comparable to those in astrocytes. Immunocytochemistry showed significant GSH staining in neurones. Fluorescent double staining of GSH and tyrosine hydroxylase revealed that dopaminergic neurones also contained GSH, although apparently at a lower level than other mesencephalic neurones.

AB - Although glutathione (GSH) is considered an important antioxidant in the brain, its cellular localization is unclear. In general, neurones are supposed to contain considerably less GSH than astrocytes. We determined biochemically and immunocytochemically the presence of GSH in cultured neurones and astrocytes from the cortex, mesencephalon and striatum. Cortical neurones contained less GSH than astrocytes whereas GSH levels in neurones from the striatum and mesencephalon were comparable to those in astrocytes. Immunocytochemistry showed significant GSH staining in neurones. Fluorescent double staining of GSH and tyrosine hydroxylase revealed that dopaminergic neurones also contained GSH, although apparently at a lower level than other mesencephalic neurones.

KW - Animals

KW - Astrocytes

KW - Brain

KW - Cells, Cultured

KW - Glutathione

KW - Immunohistochemistry

KW - Rats

KW - Rats, Wistar

KW - Journal Article

M3 - Article

VL - 7

SP - 1833

EP - 1836

JO - Neuroreport

JF - Neuroreport

SN - 0959-4965

IS - 11

ER -

Langeveld CH, Schepens E, Jongenelen CA, Stoof JC, Hjelle OP, Ottersen OP et al. Presence of glutathione immunoreactivity in cultured neurones and astrocytes. Neuroreport. 1996 Jul 29;7(11):1833-6.