Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles

Outi Keinänen, Ermei M. Mäkilä, Rici Lindgren, Helena Virtanen, Heidi Liljenbäck, Vesa Oikonen, Mirkka Sarparanta, Carla Molthoff, Albert D. Windhorst, Anne Roivainen, Jarno J. Salonen, Anu J. Airaksinen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using 18F-labeled tetrazine as a tracer. The inverse electron-demand Diels-Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.

Original languageEnglish
Pages (from-to)62-69
Number of pages8
JournalACS Omega
Volume2
Issue number1
DOIs
Publication statusPublished - 31 Jan 2017

Cite this

Keinänen, O., Mäkilä, E. M., Lindgren, R., Virtanen, H., Liljenbäck, H., Oikonen, V., ... Airaksinen, A. J. (2017). Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles. ACS Omega, 2(1), 62-69. https://doi.org/10.1021/acsomega.6b00269