Quantification of amikacin and kanamycin in serum using a simple and validated LC-MS/MS method

Jacob A Dijkstra, Marieke Gg Sturkenboom, Kai van Hateren, Remco A Koster, Ben Greijdanus, Jan-Willem C Alffenaar

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

BACKGROUND: Amikacin and kanamycin are frequently used in the treatment of multidrug-resistant TB. The current commercially available immunoassay is unable to analyze kanamycin and trough levels of amikacin. The objective was therefore to develop a LC-MS/MS method for the quantification of amikacin and kanamycin in human serum.

MATERIALS & METHODS: Using apramycin as internal standard, selectivity, accuracy, precision, recovery, matrix effects and stability were evaluated.

RESULTS: The presented LC-MS/MS method meets the recommendations of the US FDA with a low LLOQ of 250 ng/ml for amikacin and 100 ng/ml for kanamycin. No statistical significant difference was found between the LC-MS/MS method and the immunoassay of amikacin (Architect(®) assay, p = 0.501).

CONCLUSION: The low LLOQ of amikacin and the ability to analyze kanamycin makes the LC-MS/MS method the preferred method for analyzing these aminoglycosides.

Original languageEnglish
Pages (from-to)2125-33
Number of pages9
JournalBioanalysis
Volume6
Issue number16
DOIs
Publication statusPublished - Aug 2014

Cite this

Dijkstra, J. A., Sturkenboom, M. G., Hateren, K. V., Koster, R. A., Greijdanus, B., & Alffenaar, J-W. C. (2014). Quantification of amikacin and kanamycin in serum using a simple and validated LC-MS/MS method. Bioanalysis, 6(16), 2125-33. https://doi.org/10.4155/bio.14.191