Quantification of subendocardial and subepicardial blood flow using 15O-labeled water and PET: Experimental validation

Ornella Rimoldi*, Klaus P. Schäfers, Ronald Boellaard, Federico Turkheimer, Lars Stegger, Marilyn P. Law, Adriaan A. Lammerstma, Paolo G. Camici

*Corresponding author for this work

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The purpose of this study was to assess the feasibility and accuracy of quantifying subendocardial and subepicardial myocardial blood flow (MBF) and the relative coronary flow reserves (CFR) using 15O-labeled water (H2 15O) and 3-dimensional-only PET. Methods: Eight pigs were scanned with H215O and 15O-labeled carbon monoxide (C15O) after partially occluding the circumflex (n = 3) or the left anterior descending (n = 5) coronary artery, both at rest and during hyperemia induced by intravenous dipyridamole. Radioactive microspheres were injected during each of the H215O scans. Results: In a total of 256 paired measurements of MBF, ranging from 0.30 to 4.46 mL·g-1·min-1, microsphere and PET MBF were fairly well correlated. The mean difference between the 2 methods was -0.01 ± 0.52 mL·g-1·min-1 with 95% of the differences lying between the limits of agreement of -1.02 and 1.01 mL·g-1·min-1. CFR was significantly reduced (P < 0.05) in the ischemic subendocardium (PET = 1.12 ± 0.45; microspheres 5 1.09 ± 0.50; P = 0.86) and subepicardium (PET = 1.2 ± 0.35; microspheres = 1.32 ± 0.5; P = 0.39) in comparison with remote subendocardium (PET = 1.7 ± 0.62; microspheres 5 1.64 6 0.61; P 5 0.68) and subepicardium (PET = 1.79 ± 0.73; microspheres = 2.19 ± 0.86; P = 0.06). Conclusion: Dynamic measurements using H2 15O and a 3-dimensional-only PET tomograph allow regional estimates of the transmural distribution of MBF over a wide flow range, although transmural flow differences were underestimated because of the partial-volume effect. PET subendocardial and subepicardial CFR were in good agreement with the microsphere values.

Original languageEnglish
Pages (from-to)163-172
Number of pages10
JournalJournal of Nuclear Medicine
Issue number1
Publication statusPublished - 1 Jan 2006

Cite this

Rimoldi, O., Schäfers, K. P., Boellaard, R., Turkheimer, F., Stegger, L., Law, M. P., ... Camici, P. G. (2006). Quantification of subendocardial and subepicardial blood flow using 15O-labeled water and PET: Experimental validation. Journal of Nuclear Medicine, 47(1), 163-172.