Quantitative 3D analysis of tissue damage in a rat model of microembolization

Theodosia Georgakopoulou, Anne-Eva van der Wijk, Erik N. T. P. Bakker, Ed vanBavel*, Charles Majoie, Henk Marquering, Ed van Bavel, Alfons Hoekstra, Diederik Dippel, Hester Lingsma, Aad van der Lugt, Noor Samuels, Nikki Boodt, Yvo Roos, Simon de Meyer, Senna Staessens, Sarah Vandelanotte, Praneeta Konduri, Nerea Arrarte Terreros, Bastien ChopardFranck Raynaud, Remy Petkantchin, Mikhail Panteleev, Alexey Shibeko, Karim Zouaoui Boudjeltia, Vanessa Blanc-Guillemaud, Francesco Migliavacca, Gabriele Dubini, Giulia Luraghi, Jose Felix Rodriguez Matas, Sara Bridio, Patrick Mc Garry, Michael Gilvarry, Ray McCarthy, Kevin Moerman, Behrooz Fereidoonnezhad, Anushree Dwivedi, Sharon Duffy, Stephen Payne, Tamas Jozsa, Sissy Georgakopoulou, Raymond Padmos, Victor Azizi, Claire Miller, Max van der Kolk

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


There is a discrepancy between successful recanalization and good clinical outcome after endovascular treatment (EVT) in acute ischemic stroke patients. During removal of a thrombus, a shower of microemboli may release and lodge to the distal circulation. The objective of this study was to determine the extent of damage on brain tissue caused by microemboli. In a rat model of microembolization, a mixture of microsphere (MS) sizes (15, 25 and 50 µm diameter) was injected via the left internal carotid artery. A 3D image of the left hemisphere was reconstructed and a point-pattern spatial analysis was applied based on G- and K-functions to unravel the spatial correlation between MS and the induced hypoxia or infarction. We show a spatial correlation between MS and hypoxia or infarction spreading up to a distance of 1000–1500 µm. These results imply that microemboli, which individually may not always be harmful, can interact and result in local areas of hypoxia or even infarction when lodged in large numbers.
Original languageEnglish
Article number110723
JournalJournal of Biomechanics
Publication statusPublished - 9 Nov 2021
Externally publishedYes

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