Rab7a and Rab27b control secretion of endothelial microRNA through extracellular vesicles

Nicolas Jaé; David G. McEwan; Yosif Manavski; Reinier A. Boon; Stefanie Dimmeler

doi:10.1016/j.febslet.2015.08.040

Rab7a and Rab27b control secretion of endothelial microRNA through extracellular vesicles

Nicolas Jaé, David G. McEwan, Yosif Manavski, Reinier A. Boon, Stefanie Dimmeler^*

^*Corresponding author for this work

Physiology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

By transporting regulatory RNAs like microRNAs, extracellular vesicles provide a novel layer of intercellular gene regulation. However, the underlying secretory pathways and the mechanisms of cargo selection are poorly understood. Rab GTPases are central coordinators of membrane trafficking with distinct members of this family being responsible for specific transport pathways. Here we identified a vesicular export mechanism for miR-143, induced by the shear stress responsive transcription factor KLF2, and demonstrate its dependency on Rab7a/Rab27b in endothelial cells.

Original language	English
Article number	37350
Pages (from-to)	3182-3188
Number of pages	7
Journal	FEBS Letters
Volume	589
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2015.08.040
Publication status	Published - 7 Oct 2015

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10.1016/j.febslet.2015.08.040

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title = "Rab7a and Rab27b control secretion of endothelial microRNA through extracellular vesicles",

abstract = "By transporting regulatory RNAs like microRNAs, extracellular vesicles provide a novel layer of intercellular gene regulation. However, the underlying secretory pathways and the mechanisms of cargo selection are poorly understood. Rab GTPases are central coordinators of membrane trafficking with distinct members of this family being responsible for specific transport pathways. Here we identified a vesicular export mechanism for miR-143, induced by the shear stress responsive transcription factor KLF2, and demonstrate its dependency on Rab7a/Rab27b in endothelial cells.",

keywords = "Endothelial cell, Exosome, microRNA, Microvesicle, Rab protein",

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T1 - Rab7a and Rab27b control secretion of endothelial microRNA through extracellular vesicles

AU - Jaé, Nicolas

AU - McEwan, David G.

AU - Manavski, Yosif

AU - Boon, Reinier A.

AU - Dimmeler, Stefanie

PY - 2015/10/7

Y1 - 2015/10/7

N2 - By transporting regulatory RNAs like microRNAs, extracellular vesicles provide a novel layer of intercellular gene regulation. However, the underlying secretory pathways and the mechanisms of cargo selection are poorly understood. Rab GTPases are central coordinators of membrane trafficking with distinct members of this family being responsible for specific transport pathways. Here we identified a vesicular export mechanism for miR-143, induced by the shear stress responsive transcription factor KLF2, and demonstrate its dependency on Rab7a/Rab27b in endothelial cells.

AB - By transporting regulatory RNAs like microRNAs, extracellular vesicles provide a novel layer of intercellular gene regulation. However, the underlying secretory pathways and the mechanisms of cargo selection are poorly understood. Rab GTPases are central coordinators of membrane trafficking with distinct members of this family being responsible for specific transport pathways. Here we identified a vesicular export mechanism for miR-143, induced by the shear stress responsive transcription factor KLF2, and demonstrate its dependency on Rab7a/Rab27b in endothelial cells.

KW - Endothelial cell

KW - Exosome

KW - microRNA

KW - Microvesicle

KW - Rab protein

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U2 - 10.1016/j.febslet.2015.08.040

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M3 - Article

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 20

M1 - 37350

ER -

Rab7a and Rab27b control secretion of endothelial microRNA through extracellular vesicles

Abstract

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