Rapid reconstitution of CD4 T cells and NK cells protects against CMV-reactivation after allogeneic stem cell transplantation

Julia Drylewicz, Ingrid M M Schellens, Rogier Gaiser, Nening M Nanlohy, Esther D Quakkelaar, Henny Otten, Suzanne van Dorp, Ronald Jacobi, Leonie Ran, Sanne Spijkers, Dan Koning, Rob Schuurman, Ellen Meijer, Floortje L Pietersma, Jurgen Kuball, Debbie van Baarle

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

BACKGROUND: Epstein-Barr virus and Cytomegalovirus reactivations frequently occur after allogeneic stem cell transplantation (SCT).

METHODS: Here we investigated the role of immune cell reconstitution in the onset and subsequent severity of EBV- and CMV-reactivation. To this end, 116 patients were prospectively sampled for absolute T cell (CD4 and CD8), B-cell (CD19) and NK-cell (CD16 and CD56) numbers weekly post-SCT during the first 3 months and thereafter monthly until 6 months post-SCT. Viral load was monitored in parallel.

RESULTS: In contrast to the general belief, we found that early T-cell reconstitution does not play a role in the onset of viral reactivation. CMV reactivation in the first 7 weeks after SCT however resulted in higher absolute CD8(+) T-cell numbers 6 months post-SCT in patients with high-level reactivation, many of which were CMV-specific. Interestingly, rapid reconstitution of CD4(+) T-cells, as well as NK cells and the presence of donor KIR3DL1, are associated with the absence of CMV-reactivation after SCT, suggestive of a protective role of these cells. In contrast, EBV-reactivations were not affected in any way by the level of immune reconstitution after SCT.

CONCLUSION: In conclusion, these data suggest that CD4(+) T-cells and NK cells, rather than CD8(+) T-cells, are associated with protection against CMV-reactivation.

Original languageEnglish
Pages (from-to)230
JournalJournal of Translational Medicine
Volume14
Issue number1
DOIs
Publication statusPublished - 2 Aug 2016

Cite this

Drylewicz, J., Schellens, I. M. M., Gaiser, R., Nanlohy, N. M., Quakkelaar, E. D., Otten, H., ... van Baarle, D. (2016). Rapid reconstitution of CD4 T cells and NK cells protects against CMV-reactivation after allogeneic stem cell transplantation. Journal of Translational Medicine, 14(1), 230. https://doi.org/10.1186/s12967-016-0988-4