Rate of tension redevelopment is not modulated by sarcomere length in permeabilized human, murine, and porcine cardiomyocytes

István Ferenc Édes, Dániel Czuriga, Gábor Csányi, Stefan Chłopicki, Fabio A. Recchia, Attila Borbély, Zoltán Galajda, István Édes, Jolanda Van Der Velden, Ger J.M. Stienen, Zoltán Papp

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The increase in Ca2+ sensitivity of isometric force development along with sarcomere length (SL) is considered as the basis of the Frank-Starling law of the heart, possibly involving the regulation of crossbridge turnover kinetics. Therefore, the Ca2+ dependencies of isometric force production and of the cross-bridge-sensitive rate constant of force redevelopment (ktr) were determined at different SLs (1.9 and 2.3 μm) in isolated human, murine, and porcine permeabilized cardiomyocytes. ktr was also determined in the presence of 10 mM inorganic phosphate (Pi), which interfered with the force-generating cross-bridge transitions. The increases in Ca2+ sensitivities of force with SL were very similar in human, murine, and porcine cardiomyocytes (ΔpCa50: ∼0.11). ktr was higher (P < 0.05) in mice than in humans or pigs at all Ca2+ concentrations ([Ca2+]) [maximum ktr (ktr,max) at a SL of 1.9 μm and pCa 4.75: 1.33 ± 0.11, 7.44 ± 0.15, and 1.02 ± 0.05 s-1, in humans, mice, and pigs, respectively] but ktr did not depend on SL in any species. Moreover, when the ktr values for each species were expressed relative to their respective maxima, similar Ca2+ dependencies were obtained. Ten millimolar Pi decreased force to ∼60-65% and left ΔpCa50 unaltered in all three species. Pi increased ktr,max by a factor of ∼1.6 in humans and pigs and by a factor of ∼3 in mice, independent of SL. In conclusion, species differences exert a major influence on ktr, but SL does not appear to modulate the cross-bridge turnover rates in human, murine, and porcine hearts.

Original languageEnglish
Pages (from-to)R20-R29
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume293
Issue number1
DOIs
Publication statusPublished - 1 Jul 2007

Cite this

Édes, István Ferenc ; Czuriga, Dániel ; Csányi, Gábor ; Chłopicki, Stefan ; Recchia, Fabio A. ; Borbély, Attila ; Galajda, Zoltán ; Édes, István ; Van Der Velden, Jolanda ; Stienen, Ger J.M. ; Papp, Zoltán. / Rate of tension redevelopment is not modulated by sarcomere length in permeabilized human, murine, and porcine cardiomyocytes. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2007 ; Vol. 293, No. 1. pp. R20-R29.
@article{421ba321d63148989c97f38082a1942e,
title = "Rate of tension redevelopment is not modulated by sarcomere length in permeabilized human, murine, and porcine cardiomyocytes",
abstract = "The increase in Ca2+ sensitivity of isometric force development along with sarcomere length (SL) is considered as the basis of the Frank-Starling law of the heart, possibly involving the regulation of crossbridge turnover kinetics. Therefore, the Ca2+ dependencies of isometric force production and of the cross-bridge-sensitive rate constant of force redevelopment (ktr) were determined at different SLs (1.9 and 2.3 μm) in isolated human, murine, and porcine permeabilized cardiomyocytes. ktr was also determined in the presence of 10 mM inorganic phosphate (Pi), which interfered with the force-generating cross-bridge transitions. The increases in Ca2+ sensitivities of force with SL were very similar in human, murine, and porcine cardiomyocytes (ΔpCa50: ∼0.11). ktr was higher (P < 0.05) in mice than in humans or pigs at all Ca2+ concentrations ([Ca2+]) [maximum ktr (ktr,max) at a SL of 1.9 μm and pCa 4.75: 1.33 ± 0.11, 7.44 ± 0.15, and 1.02 ± 0.05 s-1, in humans, mice, and pigs, respectively] but ktr did not depend on SL in any species. Moreover, when the ktr values for each species were expressed relative to their respective maxima, similar Ca2+ dependencies were obtained. Ten millimolar Pi decreased force to ∼60-65{\%} and left ΔpCa50 unaltered in all three species. Pi increased ktr,max by a factor of ∼1.6 in humans and pigs and by a factor of ∼3 in mice, independent of SL. In conclusion, species differences exert a major influence on ktr, but SL does not appear to modulate the cross-bridge turnover rates in human, murine, and porcine hearts.",
keywords = "Calcium, Heart, Mouse, Myofilament length-dependent activation, Pig, Rate of tension redevelopment, Skinned muscle",
author = "{\'E}des, {Istv{\'a}n Ferenc} and D{\'a}niel Czuriga and G{\'a}bor Cs{\'a}nyi and Stefan Chłopicki and Recchia, {Fabio A.} and Attila Borb{\'e}ly and Zolt{\'a}n Galajda and Istv{\'a}n {\'E}des and {Van Der Velden}, Jolanda and Stienen, {Ger J.M.} and Zolt{\'a}n Papp",
year = "2007",
month = "7",
day = "1",
doi = "10.1152/ajpregu.00537.2006",
language = "English",
volume = "293",
pages = "R20--R29",
journal = "American Journal of Physiology. Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "1",

}

Rate of tension redevelopment is not modulated by sarcomere length in permeabilized human, murine, and porcine cardiomyocytes. / Édes, István Ferenc; Czuriga, Dániel; Csányi, Gábor; Chłopicki, Stefan; Recchia, Fabio A.; Borbély, Attila; Galajda, Zoltán; Édes, István; Van Der Velden, Jolanda; Stienen, Ger J.M.; Papp, Zoltán.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 293, No. 1, 01.07.2007, p. R20-R29.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Rate of tension redevelopment is not modulated by sarcomere length in permeabilized human, murine, and porcine cardiomyocytes

AU - Édes, István Ferenc

AU - Czuriga, Dániel

AU - Csányi, Gábor

AU - Chłopicki, Stefan

AU - Recchia, Fabio A.

AU - Borbély, Attila

AU - Galajda, Zoltán

AU - Édes, István

AU - Van Der Velden, Jolanda

AU - Stienen, Ger J.M.

AU - Papp, Zoltán

PY - 2007/7/1

Y1 - 2007/7/1

N2 - The increase in Ca2+ sensitivity of isometric force development along with sarcomere length (SL) is considered as the basis of the Frank-Starling law of the heart, possibly involving the regulation of crossbridge turnover kinetics. Therefore, the Ca2+ dependencies of isometric force production and of the cross-bridge-sensitive rate constant of force redevelopment (ktr) were determined at different SLs (1.9 and 2.3 μm) in isolated human, murine, and porcine permeabilized cardiomyocytes. ktr was also determined in the presence of 10 mM inorganic phosphate (Pi), which interfered with the force-generating cross-bridge transitions. The increases in Ca2+ sensitivities of force with SL were very similar in human, murine, and porcine cardiomyocytes (ΔpCa50: ∼0.11). ktr was higher (P < 0.05) in mice than in humans or pigs at all Ca2+ concentrations ([Ca2+]) [maximum ktr (ktr,max) at a SL of 1.9 μm and pCa 4.75: 1.33 ± 0.11, 7.44 ± 0.15, and 1.02 ± 0.05 s-1, in humans, mice, and pigs, respectively] but ktr did not depend on SL in any species. Moreover, when the ktr values for each species were expressed relative to their respective maxima, similar Ca2+ dependencies were obtained. Ten millimolar Pi decreased force to ∼60-65% and left ΔpCa50 unaltered in all three species. Pi increased ktr,max by a factor of ∼1.6 in humans and pigs and by a factor of ∼3 in mice, independent of SL. In conclusion, species differences exert a major influence on ktr, but SL does not appear to modulate the cross-bridge turnover rates in human, murine, and porcine hearts.

AB - The increase in Ca2+ sensitivity of isometric force development along with sarcomere length (SL) is considered as the basis of the Frank-Starling law of the heart, possibly involving the regulation of crossbridge turnover kinetics. Therefore, the Ca2+ dependencies of isometric force production and of the cross-bridge-sensitive rate constant of force redevelopment (ktr) were determined at different SLs (1.9 and 2.3 μm) in isolated human, murine, and porcine permeabilized cardiomyocytes. ktr was also determined in the presence of 10 mM inorganic phosphate (Pi), which interfered with the force-generating cross-bridge transitions. The increases in Ca2+ sensitivities of force with SL were very similar in human, murine, and porcine cardiomyocytes (ΔpCa50: ∼0.11). ktr was higher (P < 0.05) in mice than in humans or pigs at all Ca2+ concentrations ([Ca2+]) [maximum ktr (ktr,max) at a SL of 1.9 μm and pCa 4.75: 1.33 ± 0.11, 7.44 ± 0.15, and 1.02 ± 0.05 s-1, in humans, mice, and pigs, respectively] but ktr did not depend on SL in any species. Moreover, when the ktr values for each species were expressed relative to their respective maxima, similar Ca2+ dependencies were obtained. Ten millimolar Pi decreased force to ∼60-65% and left ΔpCa50 unaltered in all three species. Pi increased ktr,max by a factor of ∼1.6 in humans and pigs and by a factor of ∼3 in mice, independent of SL. In conclusion, species differences exert a major influence on ktr, but SL does not appear to modulate the cross-bridge turnover rates in human, murine, and porcine hearts.

KW - Calcium

KW - Heart

KW - Mouse

KW - Myofilament length-dependent activation

KW - Pig

KW - Rate of tension redevelopment

KW - Skinned muscle

UR - http://www.scopus.com/inward/record.url?scp=34447642662&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00537.2006

DO - 10.1152/ajpregu.00537.2006

M3 - Article

VL - 293

SP - R20-R29

JO - American Journal of Physiology. Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology. Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 1

ER -