Background: After transmural myocardial infarction (MI), changes occur in intramural myocardial function. This has been described in anterior MI only. The aim of this study was to determine the relation between variable infarct locations and intramural deformation in patients with a first MI. Methods: Forty patients (33 men and 7 women aged 57 ± 11 years) with different infarct-related coronary arteries (25 left anterior descending, 7 circumflex, and 8 right coronary) were studied 6 ± 3 days after infarction with magnetic resonance tissue tagging and 2-dimensional finite element analysis of myocardial deformation. Short-axis tagged images were acquired at base, mid, and apical level. Intramural deformation was measured in 6 circumferential segments per level. Results were compared with 9 age-matched healthy controls. Results: Each infarct area demonstrated a significant reduction of intramural deformation. At mid-ventricular level, segments with maximum impaired intramural function were the anteroseptal segment for left anterior descending-related MI (stretch: 16% vs 33% for controls, P < .001), the posterolateral segment for related MI (stretch: 20% vs 34%, P < .01); and the inferior segment for right coronary artery related MI (stretch: 18% vs 25%, P = .082). In these infarct segments, the intramural regional systolic stretch was more circumferentially oriented compared with radially oriented stretch in the same segments in controls (P < .05). Conclusion: The infarct area can be recognized by a specific spatial pattern of intramural deformation. In infarcted compared with noninfarcted myocardium, deformation is significantly reduced and systolic stretch deviates from the radial direction. Left anterior descending related infarcts were found to have larger regional differences in intramural deformation than circumflex or right coronary artery related MI of enzymatically the same size.