Recovery of T cell subsets after autologous bone marrow transplantation is mainly due to proliferation of mature T cells in the graft

G C de Gast, L F Verdonck, J M Middeldorp, T H The, A Hekker, J A vd Linden, H A Kreeft, B J Bast

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In 22 patients with malignancies, treated with high-dose chemoradiotherapy and autologous bone marrow transplantation (BMT), peripheral blood T cell subsets and functions were studied. In ten cytomegalovirus (CMV)-negative patients, CD4+ and CD8+ T cells (representing T cells of the helper/inducer phenotype and T cells of the suppressor/cytotoxic phenotype, respectively), recovered slowly and simultaneously. In 12 CMV-positive patients, however, CD8+ T cells recovered more rapidly than CD4+ T cells and rose to increased counts. No T cells with an immature phenotype (CD1+, OKT6+) were observed. Lymphocyte stimulation by herpes simplex virus infected fibroblasts (and by CMV-infected fibroblasts in CMV-positive patients) in contrast remained high and even increased after BMT in both groups. These data indicate that T cell recovery after autologous BMT is mainly due to proliferation of mature T cells present in the BM graft and not to generation of new T cells from T cell precursors.

Original languageEnglish
Pages (from-to)428-31
Number of pages4
JournalBlood
Volume66
Issue number2
Publication statusPublished - Aug 1985

Cite this

de Gast, G. C., Verdonck, L. F., Middeldorp, J. M., The, T. H., Hekker, A., vd Linden, J. A., ... Bast, B. J. (1985). Recovery of T cell subsets after autologous bone marrow transplantation is mainly due to proliferation of mature T cells in the graft. Blood, 66(2), 428-31.
de Gast, G C ; Verdonck, L F ; Middeldorp, J M ; The, T H ; Hekker, A ; vd Linden, J A ; Kreeft, H A ; Bast, B J. / Recovery of T cell subsets after autologous bone marrow transplantation is mainly due to proliferation of mature T cells in the graft. In: Blood. 1985 ; Vol. 66, No. 2. pp. 428-31.
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title = "Recovery of T cell subsets after autologous bone marrow transplantation is mainly due to proliferation of mature T cells in the graft",
abstract = "In 22 patients with malignancies, treated with high-dose chemoradiotherapy and autologous bone marrow transplantation (BMT), peripheral blood T cell subsets and functions were studied. In ten cytomegalovirus (CMV)-negative patients, CD4+ and CD8+ T cells (representing T cells of the helper/inducer phenotype and T cells of the suppressor/cytotoxic phenotype, respectively), recovered slowly and simultaneously. In 12 CMV-positive patients, however, CD8+ T cells recovered more rapidly than CD4+ T cells and rose to increased counts. No T cells with an immature phenotype (CD1+, OKT6+) were observed. Lymphocyte stimulation by herpes simplex virus infected fibroblasts (and by CMV-infected fibroblasts in CMV-positive patients) in contrast remained high and even increased after BMT in both groups. These data indicate that T cell recovery after autologous BMT is mainly due to proliferation of mature T cells present in the BM graft and not to generation of new T cells from T cell precursors.",
keywords = "Antibodies, Viral, Antineoplastic Combined Chemotherapy Protocols, Bone Marrow, Bone Marrow Transplantation, Cell Division, Cells, Cultured, Cytomegalovirus, Cytomegalovirus Infections, Follow-Up Studies, Humans, Lymphocyte Activation, Neoplasms, Simplexvirus, T-Lymphocytes, Whole-Body Irradiation, Journal Article, Research Support, Non-U.S. Gov't",
author = "{de Gast}, {G C} and Verdonck, {L F} and Middeldorp, {J M} and The, {T H} and A Hekker and {vd Linden}, {J A} and Kreeft, {H A} and Bast, {B J}",
year = "1985",
month = "8",
language = "English",
volume = "66",
pages = "428--31",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
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de Gast, GC, Verdonck, LF, Middeldorp, JM, The, TH, Hekker, A, vd Linden, JA, Kreeft, HA & Bast, BJ 1985, 'Recovery of T cell subsets after autologous bone marrow transplantation is mainly due to proliferation of mature T cells in the graft' Blood, vol. 66, no. 2, pp. 428-31.

Recovery of T cell subsets after autologous bone marrow transplantation is mainly due to proliferation of mature T cells in the graft. / de Gast, G C; Verdonck, L F; Middeldorp, J M; The, T H; Hekker, A; vd Linden, J A; Kreeft, H A; Bast, B J.

In: Blood, Vol. 66, No. 2, 08.1985, p. 428-31.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Recovery of T cell subsets after autologous bone marrow transplantation is mainly due to proliferation of mature T cells in the graft

AU - de Gast, G C

AU - Verdonck, L F

AU - Middeldorp, J M

AU - The, T H

AU - Hekker, A

AU - vd Linden, J A

AU - Kreeft, H A

AU - Bast, B J

PY - 1985/8

Y1 - 1985/8

N2 - In 22 patients with malignancies, treated with high-dose chemoradiotherapy and autologous bone marrow transplantation (BMT), peripheral blood T cell subsets and functions were studied. In ten cytomegalovirus (CMV)-negative patients, CD4+ and CD8+ T cells (representing T cells of the helper/inducer phenotype and T cells of the suppressor/cytotoxic phenotype, respectively), recovered slowly and simultaneously. In 12 CMV-positive patients, however, CD8+ T cells recovered more rapidly than CD4+ T cells and rose to increased counts. No T cells with an immature phenotype (CD1+, OKT6+) were observed. Lymphocyte stimulation by herpes simplex virus infected fibroblasts (and by CMV-infected fibroblasts in CMV-positive patients) in contrast remained high and even increased after BMT in both groups. These data indicate that T cell recovery after autologous BMT is mainly due to proliferation of mature T cells present in the BM graft and not to generation of new T cells from T cell precursors.

AB - In 22 patients with malignancies, treated with high-dose chemoradiotherapy and autologous bone marrow transplantation (BMT), peripheral blood T cell subsets and functions were studied. In ten cytomegalovirus (CMV)-negative patients, CD4+ and CD8+ T cells (representing T cells of the helper/inducer phenotype and T cells of the suppressor/cytotoxic phenotype, respectively), recovered slowly and simultaneously. In 12 CMV-positive patients, however, CD8+ T cells recovered more rapidly than CD4+ T cells and rose to increased counts. No T cells with an immature phenotype (CD1+, OKT6+) were observed. Lymphocyte stimulation by herpes simplex virus infected fibroblasts (and by CMV-infected fibroblasts in CMV-positive patients) in contrast remained high and even increased after BMT in both groups. These data indicate that T cell recovery after autologous BMT is mainly due to proliferation of mature T cells present in the BM graft and not to generation of new T cells from T cell precursors.

KW - Antibodies, Viral

KW - Antineoplastic Combined Chemotherapy Protocols

KW - Bone Marrow

KW - Bone Marrow Transplantation

KW - Cell Division

KW - Cells, Cultured

KW - Cytomegalovirus

KW - Cytomegalovirus Infections

KW - Follow-Up Studies

KW - Humans

KW - Lymphocyte Activation

KW - Neoplasms

KW - Simplexvirus

KW - T-Lymphocytes

KW - Whole-Body Irradiation

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 66

SP - 428

EP - 431

JO - Blood

JF - Blood

SN - 0006-4971

IS - 2

ER -