TY - JOUR
T1 - Recruitment of antigen-specific CD8+ T cells in response to infection is markedly efficient
AU - van Heijst, Jeroen W J
AU - Gerlach, Carmen
AU - Swart, Erwin
AU - Sie, Daoud
AU - Nunes-Alves, Cláudio
AU - Kerkhoven, Ron M
AU - Arens, Ramon
AU - Correia-Neves, Margarida
AU - Schepers, Koen
AU - Schumacher, Ton N M
PY - 2009/9/4
Y1 - 2009/9/4
N2 - The magnitude of antigen-specific CD8+ T cell responses is not fixed but correlates with the severity of infection. Although by definition T cell response size is the product of both the capacity to recruit naïve T cells (clonal selection) and their subsequent proliferation (clonal expansion), it remains undefined how these two factors regulate antigen-specific T cell responses. We determined the relative contribution of recruitment and expansion by labeling naïve T cells with unique genetic tags and transferring them into mice. Under disparate infection conditions with different pathogens and doses, recruitment of antigen-specific T cells was near constant and close to complete. Thus, naïve T cell recruitment is highly efficient, and the magnitude of antigen-specific CD8+ T cell responses is primarily controlled by clonal expansion.
AB - The magnitude of antigen-specific CD8+ T cell responses is not fixed but correlates with the severity of infection. Although by definition T cell response size is the product of both the capacity to recruit naïve T cells (clonal selection) and their subsequent proliferation (clonal expansion), it remains undefined how these two factors regulate antigen-specific T cell responses. We determined the relative contribution of recruitment and expansion by labeling naïve T cells with unique genetic tags and transferring them into mice. Under disparate infection conditions with different pathogens and doses, recruitment of antigen-specific T cells was near constant and close to complete. Thus, naïve T cell recruitment is highly efficient, and the magnitude of antigen-specific CD8+ T cell responses is primarily controlled by clonal expansion.
KW - Adoptive Transfer
KW - Ampicillin/therapeutic use
KW - Animals
KW - Anti-Bacterial Agents/therapeutic use
KW - Antigens/immunology
KW - Antigens, Bacterial/immunology
KW - Antigens, Viral/immunology
KW - CD8-Positive T-Lymphocytes/immunology
KW - Dendritic Cells/immunology
KW - Epitopes/immunology
KW - Genes, T-Cell Receptor alpha
KW - Genes, T-Cell Receptor beta
KW - Influenza A virus/immunology
KW - Listeriosis/drug therapy
KW - Lymphocyte Activation
KW - Lymphocyte Count
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Transgenic
KW - Molecular Sequence Data
KW - Orthomyxoviridae Infections/immunology
KW - Ovalbumin/immunology
KW - Receptors, Antigen, T-Cell, alpha-beta/chemistry
KW - Spleen/immunology
KW - Vaccinia/immunology
KW - Virus Diseases/immunology
U2 - 10.1126/science.1175455
DO - 10.1126/science.1175455
M3 - Article
C2 - 19729659
VL - 325
SP - 1265
EP - 1269
JO - Science
JF - Science
SN - 0036-8075
IS - 5945
ER -