TY - JOUR
T1 - Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE
AU - Ledeboer, Annemarie
AU - Wierinckx, Anne
AU - Bol, John G J M
AU - Floris, Sarah
AU - Renardel de Lavalette, Chantal
AU - De Vries, Helga E
AU - van den Berg, Timo K
AU - Dijkstra, Christine D
AU - Tilders, Fred J H
AU - van dam, Anne-Marie
PY - 2003/3
Y1 - 2003/3
N2 - Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mediate leukocyte infiltration into the CNS, in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Because exogenous interleukin-10 (IL-10) inhibits ICAM-1 and VCAM-1 expression and clinical EAE, we hypothesize that endogenous IL-10 signaling may suppress expression of adhesion molecules. In a rat model of chronic relapsing EAE, expression levels of IL-10 and its receptor (IL-10R1), ICAM-1 and VCAM-1 mRNA in the spinal cord are markedly increased, whereas levels of IL-10 mRNA remain relatively low. The temporal pattern of mRNA and protein expression showed marked differences between spinal cord levels. During relapse, IL-10, IL-10R1, ICAM-1, VCAM-1 mRNA levels and neurological scores show positive correlations. We conclude that endogenous IL-10 is not a crucial factor inhibiting adhesion molecule expression in this model.
AB - Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mediate leukocyte infiltration into the CNS, in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Because exogenous interleukin-10 (IL-10) inhibits ICAM-1 and VCAM-1 expression and clinical EAE, we hypothesize that endogenous IL-10 signaling may suppress expression of adhesion molecules. In a rat model of chronic relapsing EAE, expression levels of IL-10 and its receptor (IL-10R1), ICAM-1 and VCAM-1 mRNA in the spinal cord are markedly increased, whereas levels of IL-10 mRNA remain relatively low. The temporal pattern of mRNA and protein expression showed marked differences between spinal cord levels. During relapse, IL-10, IL-10R1, ICAM-1, VCAM-1 mRNA levels and neurological scores show positive correlations. We conclude that endogenous IL-10 is not a crucial factor inhibiting adhesion molecule expression in this model.
KW - Animals
KW - Cell Adhesion Molecules
KW - Chemotaxis, Leukocyte
KW - Chronic Disease
KW - Disease Models, Animal
KW - Encephalomyelitis, Autoimmune, Experimental
KW - Gene Expression Regulation
KW - Immunohistochemistry
KW - Intercellular Adhesion Molecule-1
KW - Interleukin-10
KW - Male
KW - Multiple Sclerosis, Relapsing-Remitting
KW - RNA, Messenger
KW - Rats
KW - Rats, Inbred Strains
KW - Reaction Time
KW - Receptors, Interleukin
KW - Receptors, Interleukin-10
KW - Spinal Cord
KW - Time Factors
KW - Vascular Cell Adhesion Molecule-1
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Article
C2 - 12620647
VL - 136
SP - 94
EP - 103
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -