Regulation of Phagocyte Migration by Signal Regulatory Protein-Alpha Signaling

Julian Alvarez-Zarate, Hanke L Matlung, Takashi Matozaki, Taco W Kuijpers, Isabelle Maridonneau-Parini, Timo K van den Berg

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Abstract

Signaling through the inhibitory receptor signal regulatory protein-alpha (SIRPα) controls effector functions in phagocytes. However, there are also indications that interactions between SIRPα and its ligand CD47 are involved in phagocyte transendothelial migration. We have investigated the involvement of SIRPα signaling in phagocyte migration in vitro and in vivo using mice that lack the SIRPα cytoplasmic tail. During thioglycolate-induced peritonitis in SIRPα mutant mice, both neutrophil and macrophage influx were found to occur, but to be significantly delayed. SIRPα signaling appeared to be essential for an optimal transendothelial migration and chemotaxis, and for the amoeboid type of phagocyte migration in 3-dimensional environments. These findings demonstrate, for the first time, that SIRPα signaling can directly control phagocyte migration, and this may contribute to the impaired inflammatory phenotype that has been observed in the absence of SIRPα signaling.

Original languageEnglish
Pages (from-to)e0127178
JournalPLoS ONE
Volume10
Issue number6
DOIs
Publication statusPublished - 2015

Cite this

Alvarez-Zarate, J., Matlung, H. L., Matozaki, T., Kuijpers, T. W., Maridonneau-Parini, I., & van den Berg, T. K. (2015). Regulation of Phagocyte Migration by Signal Regulatory Protein-Alpha Signaling. PLoS ONE, 10(6), e0127178. https://doi.org/10.1371/journal.pone.0127178