Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases

P Meij, M B Vervoort, J Aarbiou, P van Dissel, A Brink, E Bloemena, C J Meijer, J M Middeldorp

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Human antibody responses to latent membrane protein 1 (LMP1) in patients with Epstein-Barr virus (EBV)-related disease syndromes were analyzed in detail. Only by immunoblot analysis with purified recombinant LMP1 and by IFA on recombinant LMP1-expressing insect cells could human antibodies directed against LMP1 be detected. Low serum levels of LMP1-directed antibodies could be detected in 3 of 8 EBV-positive Hodgkin's disease patients, 3 of 40 nasopharyngeal carcinoma patients, 2 of 23 Burkitt's lymphoma patients, and 1 of 27 non-Burkitt's lymphoma patients. No LMP1-directed antibodies could be detected in healthy EBV carriers, infectious mononucleosis patients, or patients with chronic EBV disease. All sera contained significant levels of EBV antibodies directed against the immunodominant EBV proteins and peptides. From this study, it can be concluded that LMP1 is a protein with a very low immunogenicity for the humoral immune response in humans.

Original languageEnglish
Pages (from-to)1108-15
Number of pages8
JournalJournal of Infectious Diseases
Volume179
Issue number5
DOIs
Publication statusPublished - May 1999

Cite this

@article{3d8740fc06de49f0bfbb796c69cce666,
title = "Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases",
abstract = "Human antibody responses to latent membrane protein 1 (LMP1) in patients with Epstein-Barr virus (EBV)-related disease syndromes were analyzed in detail. Only by immunoblot analysis with purified recombinant LMP1 and by IFA on recombinant LMP1-expressing insect cells could human antibodies directed against LMP1 be detected. Low serum levels of LMP1-directed antibodies could be detected in 3 of 8 EBV-positive Hodgkin's disease patients, 3 of 40 nasopharyngeal carcinoma patients, 2 of 23 Burkitt's lymphoma patients, and 1 of 27 non-Burkitt's lymphoma patients. No LMP1-directed antibodies could be detected in healthy EBV carriers, infectious mononucleosis patients, or patients with chronic EBV disease. All sera contained significant levels of EBV antibodies directed against the immunodominant EBV proteins and peptides. From this study, it can be concluded that LMP1 is a protein with a very low immunogenicity for the humoral immune response in humans.",
keywords = "Amino Acid Sequence, Animals, Antibodies, Viral, Antigens, Viral, Burkitt Lymphoma, Enzyme-Linked Immunosorbent Assay, Epstein-Barr Virus Infections, Fluorescent Antibody Technique, Indirect, Herpesvirus 4, Human, Hodgkin Disease, Humans, Immunoblotting, Infectious Mononucleosis, Lymphoma, Mice, Molecular Sequence Data, Nasopharyngeal Neoplasms, Peptides, Recombinant Proteins, Viral Matrix Proteins, Journal Article, Research Support, Non-U.S. Gov't",
author = "P Meij and Vervoort, {M B} and J Aarbiou and {van Dissel}, P and A Brink and E Bloemena and Meijer, {C J} and Middeldorp, {J M}",
year = "1999",
month = "5",
doi = "10.1086/314704",
language = "English",
volume = "179",
pages = "1108--15",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "5",

}

Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases. / Meij, P; Vervoort, M B; Aarbiou, J; van Dissel, P; Brink, A; Bloemena, E; Meijer, C J; Middeldorp, J M.

In: Journal of Infectious Diseases, Vol. 179, No. 5, 05.1999, p. 1108-15.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Restricted low-level human antibody responses against Epstein-Barr virus (EBV)-encoded latent membrane protein 1 in a subgroup of patients with EBV-associated diseases

AU - Meij, P

AU - Vervoort, M B

AU - Aarbiou, J

AU - van Dissel, P

AU - Brink, A

AU - Bloemena, E

AU - Meijer, C J

AU - Middeldorp, J M

PY - 1999/5

Y1 - 1999/5

N2 - Human antibody responses to latent membrane protein 1 (LMP1) in patients with Epstein-Barr virus (EBV)-related disease syndromes were analyzed in detail. Only by immunoblot analysis with purified recombinant LMP1 and by IFA on recombinant LMP1-expressing insect cells could human antibodies directed against LMP1 be detected. Low serum levels of LMP1-directed antibodies could be detected in 3 of 8 EBV-positive Hodgkin's disease patients, 3 of 40 nasopharyngeal carcinoma patients, 2 of 23 Burkitt's lymphoma patients, and 1 of 27 non-Burkitt's lymphoma patients. No LMP1-directed antibodies could be detected in healthy EBV carriers, infectious mononucleosis patients, or patients with chronic EBV disease. All sera contained significant levels of EBV antibodies directed against the immunodominant EBV proteins and peptides. From this study, it can be concluded that LMP1 is a protein with a very low immunogenicity for the humoral immune response in humans.

AB - Human antibody responses to latent membrane protein 1 (LMP1) in patients with Epstein-Barr virus (EBV)-related disease syndromes were analyzed in detail. Only by immunoblot analysis with purified recombinant LMP1 and by IFA on recombinant LMP1-expressing insect cells could human antibodies directed against LMP1 be detected. Low serum levels of LMP1-directed antibodies could be detected in 3 of 8 EBV-positive Hodgkin's disease patients, 3 of 40 nasopharyngeal carcinoma patients, 2 of 23 Burkitt's lymphoma patients, and 1 of 27 non-Burkitt's lymphoma patients. No LMP1-directed antibodies could be detected in healthy EBV carriers, infectious mononucleosis patients, or patients with chronic EBV disease. All sera contained significant levels of EBV antibodies directed against the immunodominant EBV proteins and peptides. From this study, it can be concluded that LMP1 is a protein with a very low immunogenicity for the humoral immune response in humans.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Viral

KW - Antigens, Viral

KW - Burkitt Lymphoma

KW - Enzyme-Linked Immunosorbent Assay

KW - Epstein-Barr Virus Infections

KW - Fluorescent Antibody Technique, Indirect

KW - Herpesvirus 4, Human

KW - Hodgkin Disease

KW - Humans

KW - Immunoblotting

KW - Infectious Mononucleosis

KW - Lymphoma

KW - Mice

KW - Molecular Sequence Data

KW - Nasopharyngeal Neoplasms

KW - Peptides

KW - Recombinant Proteins

KW - Viral Matrix Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1086/314704

DO - 10.1086/314704

M3 - Article

VL - 179

SP - 1108

EP - 1115

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -