Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN

T B Geijtenbeek, G Koopman, G C van Duijnhoven, S J van Vliet, A C van Schijndel, A Engering, J L Heeney, Y van Kooyk

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Dendritic cells (DC) have been implicated in the pathogenesis of both human and simian immunodeficiency viruses (HIV and SIV, respectively). The DC-specific HIV-1 trans-receptor DC-SIGN is thought to be essential for viral dissemination by DC. Abundant expression in lymphoid tissues also implies a function for DC-SIGN in chronic HIV-1 infections, in facilitating persistent infection of T cells. We have therefore isolated the rhesus macaque and chimpanzee homologues of DC-SIGN to investigate their function in a primate model. Both rhesus macaque and chimpanzee DC-SIGN are highly similar to the human homologue. Three monoclonal antibodies against human DC-SIGN, AZN-D1, -D2 and -D3, cross-react with rhesus macaque DC-SIGN, whereas AZN-D2 does not cross-react with chimpanzee DC-SIGN. The primate homologues are abundantly expressed in lymphoid tissues such as lymph nodes, as well as in mucosal tissues involved in sexual transmission of HIV-1, and are functionally similar to human DC-SIGN. They have a high affinity for the immunological ligands of DC-SIGN: ICAM-2 and -3. Moreover, both homologues bind the HIV-1 envelope glycoprotein gp120 and therefore can act as a HIV-1 trans-receptor in the same way as human DC-SIGN. These data demonstrate that primate models are suitable to further dissect the role of DC-SIGN in the transmission and pathogenesis of infection with immunodeficiency viruses.

Original languageEnglish
Pages (from-to)101-7
Number of pages7
JournalImmunology Letters
Volume79
Issue number1-2
Publication statusPublished - 1 Nov 2001

Cite this

Geijtenbeek, T. B., Koopman, G., van Duijnhoven, G. C., van Vliet, S. J., van Schijndel, A. C., Engering, A., ... van Kooyk, Y. (2001). Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN. Immunology Letters, 79(1-2), 101-7.
Geijtenbeek, T B ; Koopman, G ; van Duijnhoven, G C ; van Vliet, S J ; van Schijndel, A C ; Engering, A ; Heeney, J L ; van Kooyk, Y. / Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN. In: Immunology Letters. 2001 ; Vol. 79, No. 1-2. pp. 101-7.
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abstract = "Dendritic cells (DC) have been implicated in the pathogenesis of both human and simian immunodeficiency viruses (HIV and SIV, respectively). The DC-specific HIV-1 trans-receptor DC-SIGN is thought to be essential for viral dissemination by DC. Abundant expression in lymphoid tissues also implies a function for DC-SIGN in chronic HIV-1 infections, in facilitating persistent infection of T cells. We have therefore isolated the rhesus macaque and chimpanzee homologues of DC-SIGN to investigate their function in a primate model. Both rhesus macaque and chimpanzee DC-SIGN are highly similar to the human homologue. Three monoclonal antibodies against human DC-SIGN, AZN-D1, -D2 and -D3, cross-react with rhesus macaque DC-SIGN, whereas AZN-D2 does not cross-react with chimpanzee DC-SIGN. The primate homologues are abundantly expressed in lymphoid tissues such as lymph nodes, as well as in mucosal tissues involved in sexual transmission of HIV-1, and are functionally similar to human DC-SIGN. They have a high affinity for the immunological ligands of DC-SIGN: ICAM-2 and -3. Moreover, both homologues bind the HIV-1 envelope glycoprotein gp120 and therefore can act as a HIV-1 trans-receptor in the same way as human DC-SIGN. These data demonstrate that primate models are suitable to further dissect the role of DC-SIGN in the transmission and pathogenesis of infection with immunodeficiency viruses.",
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Geijtenbeek, TB, Koopman, G, van Duijnhoven, GC, van Vliet, SJ, van Schijndel, AC, Engering, A, Heeney, JL & van Kooyk, Y 2001, 'Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN' Immunology Letters, vol. 79, no. 1-2, pp. 101-7.

Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN. / Geijtenbeek, T B; Koopman, G; van Duijnhoven, G C; van Vliet, S J; van Schijndel, A C; Engering, A; Heeney, J L; van Kooyk, Y.

In: Immunology Letters, Vol. 79, No. 1-2, 01.11.2001, p. 101-7.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN

AU - Geijtenbeek, T B

AU - Koopman, G

AU - van Duijnhoven, G C

AU - van Vliet, S J

AU - van Schijndel, A C

AU - Engering, A

AU - Heeney, J L

AU - van Kooyk, Y

PY - 2001/11/1

Y1 - 2001/11/1

N2 - Dendritic cells (DC) have been implicated in the pathogenesis of both human and simian immunodeficiency viruses (HIV and SIV, respectively). The DC-specific HIV-1 trans-receptor DC-SIGN is thought to be essential for viral dissemination by DC. Abundant expression in lymphoid tissues also implies a function for DC-SIGN in chronic HIV-1 infections, in facilitating persistent infection of T cells. We have therefore isolated the rhesus macaque and chimpanzee homologues of DC-SIGN to investigate their function in a primate model. Both rhesus macaque and chimpanzee DC-SIGN are highly similar to the human homologue. Three monoclonal antibodies against human DC-SIGN, AZN-D1, -D2 and -D3, cross-react with rhesus macaque DC-SIGN, whereas AZN-D2 does not cross-react with chimpanzee DC-SIGN. The primate homologues are abundantly expressed in lymphoid tissues such as lymph nodes, as well as in mucosal tissues involved in sexual transmission of HIV-1, and are functionally similar to human DC-SIGN. They have a high affinity for the immunological ligands of DC-SIGN: ICAM-2 and -3. Moreover, both homologues bind the HIV-1 envelope glycoprotein gp120 and therefore can act as a HIV-1 trans-receptor in the same way as human DC-SIGN. These data demonstrate that primate models are suitable to further dissect the role of DC-SIGN in the transmission and pathogenesis of infection with immunodeficiency viruses.

AB - Dendritic cells (DC) have been implicated in the pathogenesis of both human and simian immunodeficiency viruses (HIV and SIV, respectively). The DC-specific HIV-1 trans-receptor DC-SIGN is thought to be essential for viral dissemination by DC. Abundant expression in lymphoid tissues also implies a function for DC-SIGN in chronic HIV-1 infections, in facilitating persistent infection of T cells. We have therefore isolated the rhesus macaque and chimpanzee homologues of DC-SIGN to investigate their function in a primate model. Both rhesus macaque and chimpanzee DC-SIGN are highly similar to the human homologue. Three monoclonal antibodies against human DC-SIGN, AZN-D1, -D2 and -D3, cross-react with rhesus macaque DC-SIGN, whereas AZN-D2 does not cross-react with chimpanzee DC-SIGN. The primate homologues are abundantly expressed in lymphoid tissues such as lymph nodes, as well as in mucosal tissues involved in sexual transmission of HIV-1, and are functionally similar to human DC-SIGN. They have a high affinity for the immunological ligands of DC-SIGN: ICAM-2 and -3. Moreover, both homologues bind the HIV-1 envelope glycoprotein gp120 and therefore can act as a HIV-1 trans-receptor in the same way as human DC-SIGN. These data demonstrate that primate models are suitable to further dissect the role of DC-SIGN in the transmission and pathogenesis of infection with immunodeficiency viruses.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Monoclonal

KW - Base Sequence

KW - Cell Adhesion Molecules

KW - Cross Reactions

KW - DNA, Complementary/genetics

KW - Dendritic Cells/immunology

KW - Gene Expression

KW - HIV Envelope Protein gp120/immunology

KW - HIV Infections/immunology

KW - HIV-1/immunology

KW - Humans

KW - Lectins/genetics

KW - Lectins, C-Type

KW - Ligands

KW - Macaca mulatta/genetics

KW - Membrane Glycoproteins

KW - Molecular Sequence Data

KW - Pan troglodytes/genetics

KW - Receptors, Cell Surface/genetics

KW - Receptors, HIV/genetics

KW - Sequence Homology, Amino Acid

KW - Simian Immunodeficiency Virus/immunology

KW - Species Specificity

KW - Viral Envelope Proteins

M3 - Article

VL - 79

SP - 101

EP - 107

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 1-2

ER -

Geijtenbeek TB, Koopman G, van Duijnhoven GC, van Vliet SJ, van Schijndel AC, Engering A et al. Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN. Immunology Letters. 2001 Nov 1;79(1-2):101-7.