Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children

Chiara Della Bella, Elisabetta Venturini, Savannah Devente, Paola Piccini, Simona Tapinassi, Leila Bianchi, Alessia Grassi, Marisa Benagiano, Heba F. Mustafa Alnwaisri, Carlotta Montagnani, Elena Chiappini, Wilbert Bitter, Mario M. D’Elios, Maurizio de Martino, Luisa Galli

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.

Cite this

Bella, Chiara Della ; Venturini, Elisabetta ; Devente, Savannah ; Piccini, Paola ; Tapinassi, Simona ; Bianchi, Leila ; Grassi, Alessia ; Benagiano, Marisa ; Alnwaisri, Heba F. Mustafa ; Montagnani, Carlotta ; Chiappini, Elena ; Bitter, Wilbert ; D’Elios, Mario M. ; de Martino, Maurizio ; Galli, Luisa. / Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children. In: European Journal of Clinical Microbiology and Infectious Diseases. 2019.
@article{688f8b2e40a84e45b076247917f502b4,
title = "Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children",
abstract = "Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.",
author = "Bella, {Chiara Della} and Elisabetta Venturini and Savannah Devente and Paola Piccini and Simona Tapinassi and Leila Bianchi and Alessia Grassi and Marisa Benagiano and Alnwaisri, {Heba F. Mustafa} and Carlotta Montagnani and Elena Chiappini and Wilbert Bitter and D’Elios, {Mario M.} and {de Martino}, Maurizio and Luisa Galli",
year = "2019",
doi = "10.1007/s10096-019-03506-1",
language = "English",
journal = "European Journal of Clinical Microbiology and Infectious Diseases",
issn = "0934-9723",
publisher = "Springer Verlag",

}

Bella, CD, Venturini, E, Devente, S, Piccini, P, Tapinassi, S, Bianchi, L, Grassi, A, Benagiano, M, Alnwaisri, HFM, Montagnani, C, Chiappini, E, Bitter, W, D’Elios, MM, de Martino, M & Galli, L 2019, 'Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children' European Journal of Clinical Microbiology and Infectious Diseases. https://doi.org/10.1007/s10096-019-03506-1

Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children. / Bella, Chiara Della; Venturini, Elisabetta; Devente, Savannah; Piccini, Paola; Tapinassi, Simona; Bianchi, Leila; Grassi, Alessia; Benagiano, Marisa; Alnwaisri, Heba F. Mustafa; Montagnani, Carlotta; Chiappini, Elena; Bitter, Wilbert; D’Elios, Mario M.; de Martino, Maurizio; Galli, Luisa.

In: European Journal of Clinical Microbiology and Infectious Diseases, 2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children

AU - Bella, Chiara Della

AU - Venturini, Elisabetta

AU - Devente, Savannah

AU - Piccini, Paola

AU - Tapinassi, Simona

AU - Bianchi, Leila

AU - Grassi, Alessia

AU - Benagiano, Marisa

AU - Alnwaisri, Heba F. Mustafa

AU - Montagnani, Carlotta

AU - Chiappini, Elena

AU - Bitter, Wilbert

AU - D’Elios, Mario M.

AU - de Martino, Maurizio

AU - Galli, Luisa

PY - 2019

Y1 - 2019

N2 - Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.

AB - Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.

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UR - https://www.ncbi.nlm.nih.gov/pubmed/30834995

U2 - 10.1007/s10096-019-03506-1

DO - 10.1007/s10096-019-03506-1

M3 - Article

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

SN - 0934-9723

ER -