Role of protein phosphorylation in the degranulation of electropermeabilized human neutrophils

H W Niessen, A J Verhoeven

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We have studied the role of protein phosphorylation in the degranulation response of human neutrophils by measuring the effect of ATP depletion and the addition of the protein kinase inhibitor staurosporine in electropermeabilized human neutrophils, activated with Ca2+ and/or GTP-gamma-S. Our studies were carried out in the presence of cytochalasin B to prevent inhibitory effects of actin polymerization on the degranulation response. It was found that protein phosphorylation plays an important role in the degranulation response in cells stimulated with the single stimuli Ca2+ or GTP-gamma-S. However, in neutrophils stimulated with the combination of these activators degranulation can occur without the apparent need for protein phosphorylation, albeit with a slower rate than with protein phosphorylation.

Original languageEnglish
Pages (from-to)267-73
Number of pages7
JournalBiochimica et Biophysica Acta. Reviews on Cancer
Volume1223
Issue number2
Publication statusPublished - 8 Sep 1994

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