Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule

T K van den Berg, J J Brevé, J G Damoiseaux, E A Döpp, S Kelm, P R Crocker, C D Dijkstra, G Kraal

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In this study we present evidence that the mouse and rat sialoadhesin (originally named sheep erythrocyte receptor) on macrophages can function as a lymphocyte adhesion molecule. Lymphocytes were shown to bind to the splenic marginal zone, and lymph node subcapsular sinus and medulla in a frozen section assay. Selective depletion experiments showed that binding was mediated by macrophages. Adhesion was blocked by preincubation of the sections with monoclonal antibodies against mouse or rat sialoadhesin. Binding was temperature dependent, divalent cation independent, and involved sialic acid residues on the lymphocyte, as it could be inhibited by prior neuraminidase treatment or addition of the ganglioside GD1a. Binding to sialoadhesin was confirmed using the purified receptor and was observed among T cells, T blasts, B cells, and B blasts. Isolated macrophages or dendritic cells showed little binding. Sialoadhesin provides the first example of a macrophage-restricted lymphocyte adhesion molecule.

Original languageEnglish
Pages (from-to)647-55
Number of pages9
JournalJournal of Experimental Medicine
Volume176
Issue number3
Publication statusPublished - 1 Sep 1992

Cite this

van den Berg, T. K., Brevé, J. J., Damoiseaux, J. G., Döpp, E. A., Kelm, S., Crocker, P. R., ... Kraal, G. (1992). Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule. Journal of Experimental Medicine, 176(3), 647-55.
van den Berg, T K ; Brevé, J J ; Damoiseaux, J G ; Döpp, E A ; Kelm, S ; Crocker, P R ; Dijkstra, C D ; Kraal, G. / Sialoadhesin on macrophages : its identification as a lymphocyte adhesion molecule. In: Journal of Experimental Medicine. 1992 ; Vol. 176, No. 3. pp. 647-55.
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abstract = "In this study we present evidence that the mouse and rat sialoadhesin (originally named sheep erythrocyte receptor) on macrophages can function as a lymphocyte adhesion molecule. Lymphocytes were shown to bind to the splenic marginal zone, and lymph node subcapsular sinus and medulla in a frozen section assay. Selective depletion experiments showed that binding was mediated by macrophages. Adhesion was blocked by preincubation of the sections with monoclonal antibodies against mouse or rat sialoadhesin. Binding was temperature dependent, divalent cation independent, and involved sialic acid residues on the lymphocyte, as it could be inhibited by prior neuraminidase treatment or addition of the ganglioside GD1a. Binding to sialoadhesin was confirmed using the purified receptor and was observed among T cells, T blasts, B cells, and B blasts. Isolated macrophages or dendritic cells showed little binding. Sialoadhesin provides the first example of a macrophage-restricted lymphocyte adhesion molecule.",
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van den Berg, TK, Brevé, JJ, Damoiseaux, JG, Döpp, EA, Kelm, S, Crocker, PR, Dijkstra, CD & Kraal, G 1992, 'Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule' Journal of Experimental Medicine, vol. 176, no. 3, pp. 647-55.

Sialoadhesin on macrophages : its identification as a lymphocyte adhesion molecule. / van den Berg, T K; Brevé, J J; Damoiseaux, J G; Döpp, E A; Kelm, S; Crocker, P R; Dijkstra, C D; Kraal, G.

In: Journal of Experimental Medicine, Vol. 176, No. 3, 01.09.1992, p. 647-55.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Sialoadhesin on macrophages

T2 - its identification as a lymphocyte adhesion molecule

AU - van den Berg, T K

AU - Brevé, J J

AU - Damoiseaux, J G

AU - Döpp, E A

AU - Kelm, S

AU - Crocker, P R

AU - Dijkstra, C D

AU - Kraal, G

PY - 1992/9/1

Y1 - 1992/9/1

N2 - In this study we present evidence that the mouse and rat sialoadhesin (originally named sheep erythrocyte receptor) on macrophages can function as a lymphocyte adhesion molecule. Lymphocytes were shown to bind to the splenic marginal zone, and lymph node subcapsular sinus and medulla in a frozen section assay. Selective depletion experiments showed that binding was mediated by macrophages. Adhesion was blocked by preincubation of the sections with monoclonal antibodies against mouse or rat sialoadhesin. Binding was temperature dependent, divalent cation independent, and involved sialic acid residues on the lymphocyte, as it could be inhibited by prior neuraminidase treatment or addition of the ganglioside GD1a. Binding to sialoadhesin was confirmed using the purified receptor and was observed among T cells, T blasts, B cells, and B blasts. Isolated macrophages or dendritic cells showed little binding. Sialoadhesin provides the first example of a macrophage-restricted lymphocyte adhesion molecule.

AB - In this study we present evidence that the mouse and rat sialoadhesin (originally named sheep erythrocyte receptor) on macrophages can function as a lymphocyte adhesion molecule. Lymphocytes were shown to bind to the splenic marginal zone, and lymph node subcapsular sinus and medulla in a frozen section assay. Selective depletion experiments showed that binding was mediated by macrophages. Adhesion was blocked by preincubation of the sections with monoclonal antibodies against mouse or rat sialoadhesin. Binding was temperature dependent, divalent cation independent, and involved sialic acid residues on the lymphocyte, as it could be inhibited by prior neuraminidase treatment or addition of the ganglioside GD1a. Binding to sialoadhesin was confirmed using the purified receptor and was observed among T cells, T blasts, B cells, and B blasts. Isolated macrophages or dendritic cells showed little binding. Sialoadhesin provides the first example of a macrophage-restricted lymphocyte adhesion molecule.

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KW - Blotting, Western

KW - Cell Adhesion Molecules/analysis

KW - Cells, Cultured

KW - Immunoenzyme Techniques

KW - Lymphocytes/cytology

KW - Macrophages/chemistry

KW - Male

KW - Membrane Glycoproteins

KW - Mice

KW - Mice, Inbred BALB C

KW - Organ Specificity

KW - Rats

KW - Rats, Inbred Strains

KW - Receptors, Immunologic/analysis

KW - Sialic Acid Binding Ig-like Lectin 1

M3 - Article

VL - 176

SP - 647

EP - 655

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 3

ER -