Signal-regulatory protein is selectively expressed by myeloid and neuronal cells

S Adams, L J van der Laan, E Vernon-Wilson, C Renardel de Lavalette, E A Döpp, C D Dijkstra, D L Simmons, T K van den Berg

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Signal-regulatory proteins (SIRP) are transmembrane glycoproteins with three extracellular Ig-like domains, closely related to Ag receptors Ig, TCR, and MHC, and a cytoplasmic domain with two immunoreceptor with tyrosine-based inhibition motifs that can interact with src homology 2 domain-containing phosphatases. SIRP have previously been shown to inhibit signaling through receptor tyrosine kinases, but their physiologic function is unknown. Here we demonstrate by expression cloning that the mAbs ED9, ED17, and MRC-OX41 recognize rat SIRP. In addition, we show for the first time that rat SIRP is selectively expressed by myeloid cells (macrophages, monocytes, granulocytes, dendritic cells) and neurons. Moreover, SIRP ligation induces nitric oxide production by macrophages. This implicates SIRP as a putative recognition/signaling receptor in both immune and nervous systems.

Original languageEnglish
Pages (from-to)1853-9
Number of pages7
JournalJournal of Immunology
Volume161
Issue number4
Publication statusPublished - 15 Aug 1998

Cite this

Adams, S., van der Laan, L. J., Vernon-Wilson, E., Renardel de Lavalette, C., Döpp, E. A., Dijkstra, C. D., ... van den Berg, T. K. (1998). Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. Journal of Immunology, 161(4), 1853-9.
Adams, S ; van der Laan, L J ; Vernon-Wilson, E ; Renardel de Lavalette, C ; Döpp, E A ; Dijkstra, C D ; Simmons, D L ; van den Berg, T K. / Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. In: Journal of Immunology. 1998 ; Vol. 161, No. 4. pp. 1853-9.
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abstract = "Signal-regulatory proteins (SIRP) are transmembrane glycoproteins with three extracellular Ig-like domains, closely related to Ag receptors Ig, TCR, and MHC, and a cytoplasmic domain with two immunoreceptor with tyrosine-based inhibition motifs that can interact with src homology 2 domain-containing phosphatases. SIRP have previously been shown to inhibit signaling through receptor tyrosine kinases, but their physiologic function is unknown. Here we demonstrate by expression cloning that the mAbs ED9, ED17, and MRC-OX41 recognize rat SIRP. In addition, we show for the first time that rat SIRP is selectively expressed by myeloid cells (macrophages, monocytes, granulocytes, dendritic cells) and neurons. Moreover, SIRP ligation induces nitric oxide production by macrophages. This implicates SIRP as a putative recognition/signaling receptor in both immune and nervous systems.",
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author = "S Adams and {van der Laan}, {L J} and E Vernon-Wilson and {Renardel de Lavalette}, C and D{\"o}pp, {E A} and Dijkstra, {C D} and Simmons, {D L} and {van den Berg}, {T K}",
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Adams, S, van der Laan, LJ, Vernon-Wilson, E, Renardel de Lavalette, C, Döpp, EA, Dijkstra, CD, Simmons, DL & van den Berg, TK 1998, 'Signal-regulatory protein is selectively expressed by myeloid and neuronal cells' Journal of Immunology, vol. 161, no. 4, pp. 1853-9.

Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. / Adams, S; van der Laan, L J; Vernon-Wilson, E; Renardel de Lavalette, C; Döpp, E A; Dijkstra, C D; Simmons, D L; van den Berg, T K.

In: Journal of Immunology, Vol. 161, No. 4, 15.08.1998, p. 1853-9.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Signal-regulatory protein is selectively expressed by myeloid and neuronal cells

AU - Adams, S

AU - van der Laan, L J

AU - Vernon-Wilson, E

AU - Renardel de Lavalette, C

AU - Döpp, E A

AU - Dijkstra, C D

AU - Simmons, D L

AU - van den Berg, T K

PY - 1998/8/15

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N2 - Signal-regulatory proteins (SIRP) are transmembrane glycoproteins with three extracellular Ig-like domains, closely related to Ag receptors Ig, TCR, and MHC, and a cytoplasmic domain with two immunoreceptor with tyrosine-based inhibition motifs that can interact with src homology 2 domain-containing phosphatases. SIRP have previously been shown to inhibit signaling through receptor tyrosine kinases, but their physiologic function is unknown. Here we demonstrate by expression cloning that the mAbs ED9, ED17, and MRC-OX41 recognize rat SIRP. In addition, we show for the first time that rat SIRP is selectively expressed by myeloid cells (macrophages, monocytes, granulocytes, dendritic cells) and neurons. Moreover, SIRP ligation induces nitric oxide production by macrophages. This implicates SIRP as a putative recognition/signaling receptor in both immune and nervous systems.

AB - Signal-regulatory proteins (SIRP) are transmembrane glycoproteins with three extracellular Ig-like domains, closely related to Ag receptors Ig, TCR, and MHC, and a cytoplasmic domain with two immunoreceptor with tyrosine-based inhibition motifs that can interact with src homology 2 domain-containing phosphatases. SIRP have previously been shown to inhibit signaling through receptor tyrosine kinases, but their physiologic function is unknown. Here we demonstrate by expression cloning that the mAbs ED9, ED17, and MRC-OX41 recognize rat SIRP. In addition, we show for the first time that rat SIRP is selectively expressed by myeloid cells (macrophages, monocytes, granulocytes, dendritic cells) and neurons. Moreover, SIRP ligation induces nitric oxide production by macrophages. This implicates SIRP as a putative recognition/signaling receptor in both immune and nervous systems.

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KW - Antigens, Differentiation

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KW - Dendritic Cells/metabolism

KW - Humans

KW - Macrophages, Alveolar/metabolism

KW - Male

KW - Membrane Glycoproteins/biosynthesis

KW - Molecular Sequence Data

KW - Neural Cell Adhesion Molecule L1

KW - Neural Cell Adhesion Molecules/biosynthesis

KW - Neurons/metabolism

KW - Nitric Oxide/biosynthesis

KW - Phagocytes/metabolism

KW - Rats

KW - Rats, Inbred Strains

KW - Receptors, Immunologic

KW - Signal Transduction/immunology

M3 - Article

VL - 161

SP - 1853

EP - 1859

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -

Adams S, van der Laan LJ, Vernon-Wilson E, Renardel de Lavalette C, Döpp EA, Dijkstra CD et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. Journal of Immunology. 1998 Aug 15;161(4):1853-9.