Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC-MS/MS

Milly E. Attema-de Jonge*, Judith M. Bekkers, Heleen M. Oudemans-van Straaten, Rolf W. Sparidans, Eric J.F. Franssen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200 μl plasma), rapid (runtime 3 min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0 mg/l using MS/MS detection) method.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Issue number1-2
Publication statusPublished - 1 Feb 2008

Cite this